The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
Previously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms corresponded to five differences already known to exist in nature between...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Nature Publishing Group
2019
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| Materias: | |
| Acceso en línea: | https://www.nature.com/articles/s41598-019-46321-6 http://hdl.handle.net/20.500.12123/6293 https://doi.org/10.1038/s41598-019-46321-6 |
| _version_ | 1855035649376124928 |
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| author | Lencina, Franco Landau, Alejandra Mabel Petterson, Maria Elizabeth Pacheco, Maria Gabriela Kobayashi, K. Prina, Alberto Raul |
| author_browse | Kobayashi, K. Landau, Alejandra Mabel Lencina, Franco Pacheco, Maria Gabriela Petterson, Maria Elizabeth Prina, Alberto Raul |
| author_facet | Lencina, Franco Landau, Alejandra Mabel Petterson, Maria Elizabeth Pacheco, Maria Gabriela Kobayashi, K. Prina, Alberto Raul |
| author_sort | Lencina, Franco |
| collection | INTA Digital |
| description | Previously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms corresponded to five differences already known to exist in nature between the rpl23 gene located in the inverted repeats (IRs) and the rpl23 pseudogene located in the large single copy region (LSC). In this investigation, polymorphisms in the rpl23 gene were verified and besides, a similar situation was found for the pseudogene in cpm seedlings. On the other hand, no polymorphisms were found in any of those loci in 40 wild type barley seedlings. Those facts and the independent occurrence of polymorphisms in the gene and pseudogene in individual seedlings suggest that the detected polymorphisms initially arose from gene conversion between gene and pseudogene. Moreover, an additional recombination process involving small recombinant segments seems to occur between the two gene copies as a consequence of their location in the IRs. These and previous results support the hypothesis that t |
| format | info:ar-repo/semantics/artículo |
| id | INTA6293 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Nature Publishing Group |
| publisherStr | Nature Publishing Group |
| record_format | dspace |
| spelling | INTA62932019-11-05T18:52:40Z The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings Lencina, Franco Landau, Alejandra Mabel Petterson, Maria Elizabeth Pacheco, Maria Gabriela Kobayashi, K. Prina, Alberto Raul Pseudogenes Chloroplasts Mutagenesis Recombination Barley Vitroplants Polymorphism Seudogenes Cloroplasto Mutagénesis Genes Recombinación Cebada Vitroplantas Polimorfismo Tilling Previously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms corresponded to five differences already known to exist in nature between the rpl23 gene located in the inverted repeats (IRs) and the rpl23 pseudogene located in the large single copy region (LSC). In this investigation, polymorphisms in the rpl23 gene were verified and besides, a similar situation was found for the pseudogene in cpm seedlings. On the other hand, no polymorphisms were found in any of those loci in 40 wild type barley seedlings. Those facts and the independent occurrence of polymorphisms in the gene and pseudogene in individual seedlings suggest that the detected polymorphisms initially arose from gene conversion between gene and pseudogene. Moreover, an additional recombination process involving small recombinant segments seems to occur between the two gene copies as a consequence of their location in the IRs. These and previous results support the hypothesis that t Instituto de Genética Fil: Lencina, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Petterson, Maria Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Kobayashi, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina 2019-11-05T18:47:14Z 2019-11-05T18:47:14Z 2019-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.nature.com/articles/s41598-019-46321-6 http://hdl.handle.net/20.500.12123/6293 2045-2322 https://doi.org/10.1038/s41598-019-46321-6 eng info:eu-repograntAgreement/INTA/PNBIO/1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Nature Publishing Group Scientific report 9 (1) : 9960. (10 July 2019) |
| spellingShingle | Pseudogenes Chloroplasts Mutagenesis Recombination Barley Vitroplants Polymorphism Seudogenes Cloroplasto Mutagénesis Genes Recombinación Cebada Vitroplantas Polimorfismo Tilling Lencina, Franco Landau, Alejandra Mabel Petterson, Maria Elizabeth Pacheco, Maria Gabriela Kobayashi, K. Prina, Alberto Raul The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings |
| title | The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings |
| title_full | The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings |
| title_fullStr | The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings |
| title_full_unstemmed | The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings |
| title_short | The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings |
| title_sort | rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings |
| topic | Pseudogenes Chloroplasts Mutagenesis Recombination Barley Vitroplants Polymorphism Seudogenes Cloroplasto Mutagénesis Genes Recombinación Cebada Vitroplantas Polimorfismo Tilling |
| url | https://www.nature.com/articles/s41598-019-46321-6 http://hdl.handle.net/20.500.12123/6293 https://doi.org/10.1038/s41598-019-46321-6 |
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