Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles
Staphylococcus aureus is one of the most frequently isolated major pathogens from intramammary infections (IMI) worldwide. The mechanisms by which S. aureus IMI are established and maintained in dairy cows involve both bacterial escape strategies and modulation of the host immune response. Moreover,...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/628 http://www.sciencedirect.com/science/article/pii/S0378113516307404 https://doi.org/10.1016/j.vetmic.2017.04.009 |
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| author | Pereyra, Elizabet Amanda Lorena Sacco, Sofía Clara Dure, Andrea Baravalle, Celina Renna, María Sol Andreotti, Carolina Soledad Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet |
| author_browse | Andreotti, Carolina Soledad Baravalle, Celina Calvinho, Luis Fernando Dallard, Bibiana Elisabet Dure, Andrea Monecke, Stefan Pereyra, Elizabet Amanda Lorena Renna, María Sol Sacco, Sofía Clara |
| author_facet | Pereyra, Elizabet Amanda Lorena Sacco, Sofía Clara Dure, Andrea Baravalle, Celina Renna, María Sol Andreotti, Carolina Soledad Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet |
| author_sort | Pereyra, Elizabet Amanda Lorena |
| collection | INTA Digital |
| description | Staphylococcus aureus is one of the most frequently isolated major pathogens from intramammary infections (IMI) worldwide. The mechanisms by which S. aureus IMI are established and maintained in dairy cows involve both bacterial escape strategies and modulation of the host immune response. Moreover, it was shown that different S. aureus strains have varying effects on the immune response. The aim of this study was to investigate the immune response in a mouse mastitis model of two S. aureus strains isolated from bovine IMI with different clinical manifestation (persistent-P or non-persistent-NP), phenotypic and genotypic profile. Both strains were
capable of establishing an IMI after 264 h post inoculation (pi). Strain A (NP) showed a more aggressive
behaviour than strain B (P) at early stages of IMI, while strain B multiplied initially at a lower rate but increased
its replication capacity from 120 h pi to the end of the study (264 h pi). Strain A triggered a stronger initial
inflammatory response compared with strain B inducing higher gene and protein expression of TLR2, NF-κB
activation and higher gene expression of IL-1α at initial stage of IMI (6–12 h pi) but inducing extensive mammary tissue damage. Immune cells response was different for each S. aureus strain throughout the course of infection, showing mammary glands inoculated with strain A greater initial immune cells stimulation compared
with strain B and then a second immune cells stimulation (from 120 to 264 h pi) represented by CR Bs.As.Sur_EEA Balcarce_Garcia_JA_Retrospective analysis of cattle poisoning in Argentina onocytesmacrophages, T and B lymphocytes, mainly stimulated by strain B, consistent with inflammatory process becoming chronic. Strain-specific pathogenicity observed underscores the importance of pathogen factors in the progression of the infectious process. These results contribute to increase the available information on hostpathogen interaction and point out for the need of further research to expand the knowledge about these interactions for developing new strategies to intervene in the IMI progress. |
| format | Artículo |
| id | INTA628 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA6282020-02-14T12:20:31Z Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles Pereyra, Elizabet Amanda Lorena Sacco, Sofía Clara Dure, Andrea Baravalle, Celina Renna, María Sol Andreotti, Carolina Soledad Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet Enfermedades de los Animales Staphylococcus aureus Respuesta Inmunológica Genotipos Mastitis Immune Response Genotypes Animal Diseases Staphylococcus aureus is one of the most frequently isolated major pathogens from intramammary infections (IMI) worldwide. The mechanisms by which S. aureus IMI are established and maintained in dairy cows involve both bacterial escape strategies and modulation of the host immune response. Moreover, it was shown that different S. aureus strains have varying effects on the immune response. The aim of this study was to investigate the immune response in a mouse mastitis model of two S. aureus strains isolated from bovine IMI with different clinical manifestation (persistent-P or non-persistent-NP), phenotypic and genotypic profile. Both strains were capable of establishing an IMI after 264 h post inoculation (pi). Strain A (NP) showed a more aggressive behaviour than strain B (P) at early stages of IMI, while strain B multiplied initially at a lower rate but increased its replication capacity from 120 h pi to the end of the study (264 h pi). Strain A triggered a stronger initial inflammatory response compared with strain B inducing higher gene and protein expression of TLR2, NF-κB activation and higher gene expression of IL-1α at initial stage of IMI (6–12 h pi) but inducing extensive mammary tissue damage. Immune cells response was different for each S. aureus strain throughout the course of infection, showing mammary glands inoculated with strain A greater initial immune cells stimulation compared with strain B and then a second immune cells stimulation (from 120 to 264 h pi) represented by CR Bs.As.Sur_EEA Balcarce_Garcia_JA_Retrospective analysis of cattle poisoning in Argentina onocytesmacrophages, T and B lymphocytes, mainly stimulated by strain B, consistent with inflammatory process becoming chronic. Strain-specific pathogenicity observed underscores the importance of pathogen factors in the progression of the infectious process. These results contribute to increase the available information on hostpathogen interaction and point out for the need of further research to expand the knowledge about these interactions for developing new strategies to intervene in the IMI progress. EEA Rafaela Fil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Sacco, Sofía C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Dure, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Renna, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Andreotti, Carolina S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina Fil: Monecke, Stefan. Institute for Medical Microbiology and Hygiene; Alemania. Alere Technologies GmbH; Alemania Fil: Calvinho, Luis Fernando. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Clínicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina 2017-07-10T18:04:20Z 2017-07-10T18:04:20Z 2017 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/628 http://www.sciencedirect.com/science/article/pii/S0378113516307404 0378-1135 https://doi.org/10.1016/j.vetmic.2017.04.009 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary Microbiology 204 : 64-76 |
| spellingShingle | Enfermedades de los Animales Staphylococcus aureus Respuesta Inmunológica Genotipos Mastitis Immune Response Genotypes Animal Diseases Pereyra, Elizabet Amanda Lorena Sacco, Sofía Clara Dure, Andrea Baravalle, Celina Renna, María Sol Andreotti, Carolina Soledad Monecke, Stefan Calvinho, Luis Fernando Dallard, Bibiana Elisabet Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles |
| title | Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles |
| title_full | Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles |
| title_fullStr | Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles |
| title_full_unstemmed | Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles |
| title_short | Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles |
| title_sort | immune response of staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles |
| topic | Enfermedades de los Animales Staphylococcus aureus Respuesta Inmunológica Genotipos Mastitis Immune Response Genotypes Animal Diseases |
| url | http://hdl.handle.net/20.500.12123/628 http://www.sciencedirect.com/science/article/pii/S0378113516307404 https://doi.org/10.1016/j.vetmic.2017.04.009 |
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