Immunization with inactivated antigens of Neospora caninum induces tolllike receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers
Neospora caninum is an obligate parasite and a major cause of abortion in cattle. Pregnancy failures appear to be associated with weak innate defences on the maternal-fetal interface during infection with N. caninum. Herein, we studied the gene expression of Toll-like receptors (TLRs) in pregnant he...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/626 http://www.sciencedirect.com/science/article/pii/S0304401717302613?via%3Dihub https://doi.org/10.1016/j.vetpar.2017.06.005 |
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| author | Marin, Maia Solange Hecker, Yanina Paola Quintana, Silvina Leunda, Maria Rosa Canton, German Jose Cobo, Eduardo Rubén Moore, Prando Dadin Odeon, Anselmo Carlos Perez, Sandra |
| author_browse | Canton, German Jose Cobo, Eduardo Rubén Hecker, Yanina Paola Leunda, Maria Rosa Marin, Maia Solange Moore, Prando Dadin Odeon, Anselmo Carlos Perez, Sandra Quintana, Silvina |
| author_facet | Marin, Maia Solange Hecker, Yanina Paola Quintana, Silvina Leunda, Maria Rosa Canton, German Jose Cobo, Eduardo Rubén Moore, Prando Dadin Odeon, Anselmo Carlos Perez, Sandra |
| author_sort | Marin, Maia Solange |
| collection | INTA Digital |
| description | Neospora caninum is an obligate parasite and a major cause of abortion in cattle. Pregnancy failures appear to be associated with weak innate defences on the maternal-fetal interface during infection with N. caninum. Herein, we studied the gene expression of Toll-like receptors (TLRs) in pregnant heifers immunized with different vaccine formulations against N. caninum before mating and then challenged the heifers with live N. caninum on day 70 of gestation. TLR7 and TLR8 expression was upregulated in the placental caruncle of infected-pregnant heifers previously exposed to live N. caninum as immunogen. However, TLR7 and 8 expression in both placenta and caruncle as well as, TLR3 and 9 expression in caruncle were upregulated when heifers were previously immunized with inactivated soluble whole antigens and recombinant NcSAG1, NcHSP20 and NcGRA7 proteins. All dams were carrying viable fetuses when they were culled at day 104 of gestation. Upregulation of TLR7 and IFNγ expression was detected in fetal spleen when their mothers where previously vaccinated with soluble
antigens and recombinant NcSAG1, NcHSP20 and NcGRA7 proteins. These studies demonstrate that soluble or recombinant NcSAG1, NcHSP20 and NcGRA7 antigens induce key TLRs expression at the maternal-fetal interface, probably triggering damaging inflammatory cellular immune responses associated with abortion. Previous infection with N. caninum seems to attenuate the innate immune response at the maternal-fetal interface, which
could favour pregnancy maintenance and perpetuation of the disease. This finding represents novel information
on how N. caninum vaccination and infection modulate TLRs expression at the placenta and fetal spleen, the
possible role in the pregnancy outcomes and transplacental transmission of the protozoa. |
| format | Artículo |
| id | INTA626 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA6262019-06-11T14:32:15Z Immunization with inactivated antigens of Neospora caninum induces tolllike receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers Marin, Maia Solange Hecker, Yanina Paola Quintana, Silvina Leunda, Maria Rosa Canton, German Jose Cobo, Eduardo Rubén Moore, Prando Dadin Odeon, Anselmo Carlos Perez, Sandra Ciencias veterinarias e higiene Antígenos Neospora Caninum Novilla Gestación Inmunización Antigens Heifers Pregnancy Immunization Neospora caninum is an obligate parasite and a major cause of abortion in cattle. Pregnancy failures appear to be associated with weak innate defences on the maternal-fetal interface during infection with N. caninum. Herein, we studied the gene expression of Toll-like receptors (TLRs) in pregnant heifers immunized with different vaccine formulations against N. caninum before mating and then challenged the heifers with live N. caninum on day 70 of gestation. TLR7 and TLR8 expression was upregulated in the placental caruncle of infected-pregnant heifers previously exposed to live N. caninum as immunogen. However, TLR7 and 8 expression in both placenta and caruncle as well as, TLR3 and 9 expression in caruncle were upregulated when heifers were previously immunized with inactivated soluble whole antigens and recombinant NcSAG1, NcHSP20 and NcGRA7 proteins. All dams were carrying viable fetuses when they were culled at day 104 of gestation. Upregulation of TLR7 and IFNγ expression was detected in fetal spleen when their mothers where previously vaccinated with soluble antigens and recombinant NcSAG1, NcHSP20 and NcGRA7 proteins. These studies demonstrate that soluble or recombinant NcSAG1, NcHSP20 and NcGRA7 antigens induce key TLRs expression at the maternal-fetal interface, probably triggering damaging inflammatory cellular immune responses associated with abortion. Previous infection with N. caninum seems to attenuate the innate immune response at the maternal-fetal interface, which could favour pregnancy maintenance and perpetuation of the disease. This finding represents novel information on how N. caninum vaccination and infection modulate TLRs expression at the placenta and fetal spleen, the possible role in the pregnancy outcomes and transplacental transmission of the protozoa. EEA Balcarce Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Canton, German José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canada Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina 2017-07-10T14:51:21Z 2017-07-10T14:51:21Z 2017 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/626 http://www.sciencedirect.com/science/article/pii/S0304401717302613?via%3Dihub 0304-4017 https://doi.org/10.1016/j.vetpar.2017.06.005 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary parasitology 243 : 12-17. (2017) |
| spellingShingle | Ciencias veterinarias e higiene Antígenos Neospora Caninum Novilla Gestación Inmunización Antigens Heifers Pregnancy Immunization Marin, Maia Solange Hecker, Yanina Paola Quintana, Silvina Leunda, Maria Rosa Canton, German Jose Cobo, Eduardo Rubén Moore, Prando Dadin Odeon, Anselmo Carlos Perez, Sandra Immunization with inactivated antigens of Neospora caninum induces tolllike receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers |
| title | Immunization with inactivated antigens of Neospora caninum induces tolllike receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers |
| title_full | Immunization with inactivated antigens of Neospora caninum induces tolllike receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers |
| title_fullStr | Immunization with inactivated antigens of Neospora caninum induces tolllike receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers |
| title_full_unstemmed | Immunization with inactivated antigens of Neospora caninum induces tolllike receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers |
| title_short | Immunization with inactivated antigens of Neospora caninum induces tolllike receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers |
| title_sort | immunization with inactivated antigens of neospora caninum induces tolllike receptors 3 7 8 and 9 in maternal fetal interface of infected pregnant heifers |
| topic | Ciencias veterinarias e higiene Antígenos Neospora Caninum Novilla Gestación Inmunización Antigens Heifers Pregnancy Immunization |
| url | http://hdl.handle.net/20.500.12123/626 http://www.sciencedirect.com/science/article/pii/S0304401717302613?via%3Dihub https://doi.org/10.1016/j.vetpar.2017.06.005 |
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