Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping
Background: Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all o...
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| Format: | Artículo |
| Language: | Inglés |
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BMC
2019
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| Online Access: | https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-2061-y http://hdl.handle.net/20.500.12123/6249 https://doi.org/10.1186/s12870-019-2061-y |
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| author | Cosson, Patrick Hastoy, Cécile Erazzu, Luis Ernesto Budeguer, Carlos Jorge Boutié, Philippe Rolin, Dominique Schurdi-Levraud, Valérie |
| author_browse | Boutié, Philippe Budeguer, Carlos Jorge Cosson, Patrick Erazzu, Luis Ernesto Hastoy, Cécile Rolin, Dominique Schurdi-Levraud, Valérie |
| author_facet | Cosson, Patrick Hastoy, Cécile Erazzu, Luis Ernesto Budeguer, Carlos Jorge Boutié, Philippe Rolin, Dominique Schurdi-Levraud, Valérie |
| author_sort | Cosson, Patrick |
| collection | INTA Digital |
| description | Background: Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all over the world, the development of new cultivars is very recent, in particular due to a colossal lack of (1) germplasm collection and breeding, (2) studies on genetic diversity and its structuring, (3) genomic tools.
Results: In this study, we developped 18 EST-SSR from 150,258 EST from The Compositae Genome Project of UC Davis (http://compgenomics.ucdavis.edu/data/). We genotyped 145 S. rebaudiana individuals, issued from thirty-one cultivars and thirty-one landraces of various origins worldwide. Markers polymorphic information content (PIC) ranged between 0.60 and 0.84. An average of 12 alleles per locus and a high observed heterozygoty of 0.69 could be observed. The landraces revealed twice as many private alleles as cultivars. The genotypes could be clustered into 3 genetic populations. The landraces were grouped in the same cluster in which the oldest cultivars “Eirete” and “MoritaIII” type are also found. The other two clusters only include cultivated genotypes. One of them revealed an original genetic variability. SG phenotypes could not discriminate the three genetic clusters but phenotyping showed a wide range of composition in terms of bitter to sweet SGs.
Conclusion: This is the first study of genetic diversity in Stevia rebaudiana involving 145 genotypes, including known cultivars as well as landrace populations of different origin. This study pointed out the structuration of S. rebaudiana germplasm and the resource of the landrace populations for genetic improvement, even on the trait of SG’s composition. |
| format | Artículo |
| id | INTA6249 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | INTA62492019-10-31T12:18:51Z Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping Cosson, Patrick Hastoy, Cécile Erazzu, Luis Ernesto Budeguer, Carlos Jorge Boutié, Philippe Rolin, Dominique Schurdi-Levraud, Valérie Stevia rebaudiana Genética Diversidad Genética (como Recurso) Germoplasma Glicosidos Genotipos Genetics Genetic Diversity (as Resource) Germplasm Glycosides Genotypes Yerba Dulce Background: Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all over the world, the development of new cultivars is very recent, in particular due to a colossal lack of (1) germplasm collection and breeding, (2) studies on genetic diversity and its structuring, (3) genomic tools. Results: In this study, we developped 18 EST-SSR from 150,258 EST from The Compositae Genome Project of UC Davis (http://compgenomics.ucdavis.edu/data/). We genotyped 145 S. rebaudiana individuals, issued from thirty-one cultivars and thirty-one landraces of various origins worldwide. Markers polymorphic information content (PIC) ranged between 0.60 and 0.84. An average of 12 alleles per locus and a high observed heterozygoty of 0.69 could be observed. The landraces revealed twice as many private alleles as cultivars. The genotypes could be clustered into 3 genetic populations. The landraces were grouped in the same cluster in which the oldest cultivars “Eirete” and “MoritaIII” type are also found. The other two clusters only include cultivated genotypes. One of them revealed an original genetic variability. SG phenotypes could not discriminate the three genetic clusters but phenotyping showed a wide range of composition in terms of bitter to sweet SGs. Conclusion: This is the first study of genetic diversity in Stevia rebaudiana involving 145 genotypes, including known cultivars as well as landrace populations of different origin. This study pointed out the structuration of S. rebaudiana germplasm and the resource of the landrace populations for genetic improvement, even on the trait of SG’s composition. EEA Famaillá Fil: Cosson, Patrick. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia Fil: Hastoy, Cécile. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia. Oviatis SA; Francia Fil: Erazzu, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Fil: Budeguer, Carlos Jorge. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Boutié, Philippe. Oviatis SA; Francia Fil: Rolin, Dominique. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia Fil: Schurdi-Levraud, Valérie. INRA-Université de Bordeaux. UMR Biologie du Fruit et Pathologie; Francia 2019-10-31T12:16:10Z 2019-10-31T12:16:10Z 2019-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-2061-y http://hdl.handle.net/20.500.12123/6249 1471-2229 https://doi.org/10.1186/s12870-019-2061-y eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC BMC Plant Biology 19 : Article number 436 (October 2019) |
| spellingShingle | Stevia rebaudiana Genética Diversidad Genética (como Recurso) Germoplasma Glicosidos Genotipos Genetics Genetic Diversity (as Resource) Germplasm Glycosides Genotypes Yerba Dulce Cosson, Patrick Hastoy, Cécile Erazzu, Luis Ernesto Budeguer, Carlos Jorge Boutié, Philippe Rolin, Dominique Schurdi-Levraud, Valérie Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping |
| title | Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping |
| title_full | Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping |
| title_fullStr | Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping |
| title_full_unstemmed | Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping |
| title_short | Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping |
| title_sort | genetic diversity and population structure of the sweet leaf herb stevia rebaudiana b cultivated and landraces germplasm assessed by est ssrs genotyping and steviol glycosides phenotyping |
| topic | Stevia rebaudiana Genética Diversidad Genética (como Recurso) Germoplasma Glicosidos Genotipos Genetics Genetic Diversity (as Resource) Germplasm Glycosides Genotypes Yerba Dulce |
| url | https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-2061-y http://hdl.handle.net/20.500.12123/6249 https://doi.org/10.1186/s12870-019-2061-y |
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