P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance
Cotton blue disease (CBD) is the most important disease present in cotton crops in South America and cotton leafroll dwarf virus (CLRDV) is the causal agent. The disease has been controlled by sowing cotton varieties resistant to CLRDV. However, in the 2009/10 growing season, an outbreak due to an a...
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| Format: | Artículo |
| Language: | Inglés |
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Wiley
2019
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| Online Access: | https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13031?af=R http://hdl.handle.net/20.500.12123/6232 https://doi.org/10.1111/ppa.13031 |
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| author | Agrofoglio, Yamila Carla Delfosse, Veronica Cecilia Casse, Maria Florencia Hopp, Horacio Esteban Bonacic Kresic, Iván Ziegler-Graff, Véronique Distefano, Ana Julia |
| author_browse | Agrofoglio, Yamila Carla Bonacic Kresic, Iván Casse, Maria Florencia Delfosse, Veronica Cecilia Distefano, Ana Julia Hopp, Horacio Esteban Ziegler-Graff, Véronique |
| author_facet | Agrofoglio, Yamila Carla Delfosse, Veronica Cecilia Casse, Maria Florencia Hopp, Horacio Esteban Bonacic Kresic, Iván Ziegler-Graff, Véronique Distefano, Ana Julia |
| author_sort | Agrofoglio, Yamila Carla |
| collection | INTA Digital |
| description | Cotton blue disease (CBD) is the most important disease present in cotton crops in South America and cotton leafroll dwarf virus (CLRDV) is the causal agent. The disease has been controlled by sowing cotton varieties resistant to CLRDV. However, in the 2009/10 growing season, an outbreak due to an atypical CLRDV isolate (CLRDV‐at) occurred in northwest Argentina. Although CLRDV and CLRDV‐at genomes are very closely related, the symptoms they produce in cotton plants are quite different. P0 is the most divergent protein between the isolates and in CLRDV is a silencing suppressor protein. This work characterized the silencing suppressor activity of the P0 protein encoded by CLRDV‐at (P0CL‐at) and evaluated its role in Cbd‐resistance break in cotton plants. It was demonstrated that P0CL‐at, despite having a mutation in the consensus of the F‐box‐like motif, was able to suppress local RNA silencing, but displayed lower activity than P0CL. P0CL and P0CL‐at showed no differences in the interaction with Gossypium hirsutum SKP1 orthologue (GSK1) and Nicotiana benthamiana SKP1 and both P0 proteins triggered destabilization of ARGONAUTE1. However, when the ability to enhance PVX symptoms was evaluated, P0CL‐at was shown to be a weaker pathogenicity factor than P0CL in N. benthamiana. Interestingly, trans‐expressed P0CL‐at enabled CLRDV to systemically infect CBD‐resistant plants, and a chimeric CLRDV‐P0CL‐at infectious clone succeeded in establishing infection in CBD‐resistant cotton varieties with symptoms resembling those produced by CLRDV‐at. These results strongly suggest that P0CL‐at is the avirulence (Avr) determinant involved in breaking cotton Cbd gene‐based resistance. |
| format | Artículo |
| id | INTA6232 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA62322019-10-29T17:49:46Z P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance Agrofoglio, Yamila Carla Delfosse, Veronica Cecilia Casse, Maria Florencia Hopp, Horacio Esteban Bonacic Kresic, Iván Ziegler-Graff, Véronique Distefano, Ana Julia Gossypium Hirsutum Polerovirus Resistencia a la Enfermedad Algodón Disease Resistance Cotton CLRDV Cotton Leafroll Dwarf Virus Cotton blue disease (CBD) is the most important disease present in cotton crops in South America and cotton leafroll dwarf virus (CLRDV) is the causal agent. The disease has been controlled by sowing cotton varieties resistant to CLRDV. However, in the 2009/10 growing season, an outbreak due to an atypical CLRDV isolate (CLRDV‐at) occurred in northwest Argentina. Although CLRDV and CLRDV‐at genomes are very closely related, the symptoms they produce in cotton plants are quite different. P0 is the most divergent protein between the isolates and in CLRDV is a silencing suppressor protein. This work characterized the silencing suppressor activity of the P0 protein encoded by CLRDV‐at (P0CL‐at) and evaluated its role in Cbd‐resistance break in cotton plants. It was demonstrated that P0CL‐at, despite having a mutation in the consensus of the F‐box‐like motif, was able to suppress local RNA silencing, but displayed lower activity than P0CL. P0CL and P0CL‐at showed no differences in the interaction with Gossypium hirsutum SKP1 orthologue (GSK1) and Nicotiana benthamiana SKP1 and both P0 proteins triggered destabilization of ARGONAUTE1. However, when the ability to enhance PVX symptoms was evaluated, P0CL‐at was shown to be a weaker pathogenicity factor than P0CL in N. benthamiana. Interestingly, trans‐expressed P0CL‐at enabled CLRDV to systemically infect CBD‐resistant plants, and a chimeric CLRDV‐P0CL‐at infectious clone succeeded in establishing infection in CBD‐resistant cotton varieties with symptoms resembling those produced by CLRDV‐at. These results strongly suggest that P0CL‐at is the avirulence (Avr) determinant involved in breaking cotton Cbd gene‐based resistance. Instituto de Biotecnología Fil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Casse, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentina Fil: Ziegler-Graff, Véronique. Université de Strasbourg. Institut de Biologie Moléculaire des Plantes; Francia Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-10-29T17:38:26Z 2019-10-29T17:38:26Z 2019-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13031?af=R http://hdl.handle.net/20.500.12123/6232 1365-3059 https://doi.org/10.1111/ppa.13031 eng info:eu-repograntAgreement/INTA/PNBIO/1131023/AR./Prospección y caracterización funcional de genes de interés biotecnológico. info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant Pathology 68 (6): 1059-1071 (Agosto 2019) |
| spellingShingle | Gossypium Hirsutum Polerovirus Resistencia a la Enfermedad Algodón Disease Resistance Cotton CLRDV Cotton Leafroll Dwarf Virus Agrofoglio, Yamila Carla Delfosse, Veronica Cecilia Casse, Maria Florencia Hopp, Horacio Esteban Bonacic Kresic, Iván Ziegler-Graff, Véronique Distefano, Ana Julia P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance |
| title | P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance |
| title_full | P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance |
| title_fullStr | P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance |
| title_full_unstemmed | P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance |
| title_short | P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance |
| title_sort | p0 protein of cotton leafroll dwarf virus atypical isolate is a weak rna silencing suppressor and the avirulence determinant that breaks the cotton cbd gene based resistance |
| topic | Gossypium Hirsutum Polerovirus Resistencia a la Enfermedad Algodón Disease Resistance Cotton CLRDV Cotton Leafroll Dwarf Virus |
| url | https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13031?af=R http://hdl.handle.net/20.500.12123/6232 https://doi.org/10.1111/ppa.13031 |
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