Plant-based vaccine candidate against Infectious bursal disease: an alternative to inactivated vaccines for breeder hens

Infectious bursal disease (IBD) is an acute, highly contagious immunosuppressive disease that affects young birds causing important economic losses in the poultry industry worldwide. Strict hygiene management together with effective vaccination programs are the most important strategies to prevent I...

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Autores principales: Lucero, Maria Soledad, Richetta, Matias Daniel, Chimeno Zoth, Silvina Andrea, Jaton, Juan, Pinto, Silvina, Canet, Zulma Edith, Berinstein, Analia, Gomez, Evangelina Raquel
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0264410X19309909
http://hdl.handle.net/20.500.12123/6231
https://doi.org/10.1016/j.vaccine.2019.07.069
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author Lucero, Maria Soledad
Richetta, Matias Daniel
Chimeno Zoth, Silvina Andrea
Jaton, Juan
Pinto, Silvina
Canet, Zulma Edith
Berinstein, Analia
Gomez, Evangelina Raquel
author_browse Berinstein, Analia
Canet, Zulma Edith
Chimeno Zoth, Silvina Andrea
Gomez, Evangelina Raquel
Jaton, Juan
Lucero, Maria Soledad
Pinto, Silvina
Richetta, Matias Daniel
author_facet Lucero, Maria Soledad
Richetta, Matias Daniel
Chimeno Zoth, Silvina Andrea
Jaton, Juan
Pinto, Silvina
Canet, Zulma Edith
Berinstein, Analia
Gomez, Evangelina Raquel
author_sort Lucero, Maria Soledad
collection INTA Digital
description Infectious bursal disease (IBD) is an acute, highly contagious immunosuppressive disease that affects young birds causing important economic losses in the poultry industry worldwide. Strict hygiene management together with effective vaccination programs are the most important strategies to prevent Infectious bursal disease virus entry in poultry production facilities. Hyperimmunisation of dams with inactivated vaccines just before the laying period provides passive immunity to the progeny that protects them during the critical first few weeks after hatching before vaccination with live attenuated virus takes place. In the present study, a safe and economic plant-based vaccine candidate against IBD intended for breeder hens was evaluated. We demonstrated that the recombinant immunogen is effective as booster for previously primed hens since it increases specific antibodies against VP2 that are transmitted to the offspring with titres and decay rate similar to those achieved by inactivated vaccine. Moreover, these maternally derived antibodies have virus neutralising activity and are able to confer protection against challenge in progeny, as evidenced by absence of bursal damage and low viral titres in this organ. Taking into account the disadvantages of inactivated vaccines as well as the benefits of plants as expression systems, such as time and cost efficiency, lower risk of contamination from animal pathogens and nearly unlimited scalability, a plant-based subunit IBD vaccine represents a viable alternative in the veterinary field.
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spelling INTA62312019-10-29T15:22:28Z Plant-based vaccine candidate against Infectious bursal disease: an alternative to inactivated vaccines for breeder hens Lucero, Maria Soledad Richetta, Matias Daniel Chimeno Zoth, Silvina Andrea Jaton, Juan Pinto, Silvina Canet, Zulma Edith Berinstein, Analia Gomez, Evangelina Raquel Infectious Bursal Disease Virus Maternal Immunity Hens Vaccines Virus Bursitis Infecciosa Inmunidad Maternal Gallinas Vacuna Infectious bursal disease (IBD) is an acute, highly contagious immunosuppressive disease that affects young birds causing important economic losses in the poultry industry worldwide. Strict hygiene management together with effective vaccination programs are the most important strategies to prevent Infectious bursal disease virus entry in poultry production facilities. Hyperimmunisation of dams with inactivated vaccines just before the laying period provides passive immunity to the progeny that protects them during the critical first few weeks after hatching before vaccination with live attenuated virus takes place. In the present study, a safe and economic plant-based vaccine candidate against IBD intended for breeder hens was evaluated. We demonstrated that the recombinant immunogen is effective as booster for previously primed hens since it increases specific antibodies against VP2 that are transmitted to the offspring with titres and decay rate similar to those achieved by inactivated vaccine. Moreover, these maternally derived antibodies have virus neutralising activity and are able to confer protection against challenge in progeny, as evidenced by absence of bursal damage and low viral titres in this organ. Taking into account the disadvantages of inactivated vaccines as well as the benefits of plants as expression systems, such as time and cost efficiency, lower risk of contamination from animal pathogens and nearly unlimited scalability, a plant-based subunit IBD vaccine represents a viable alternative in the veterinary field. Instituto de Biotecnología Fil: Lucero, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Richetta, Matias Daniel.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Jaton, Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Pinto, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología; Argentina Fil: Canet, Zulma Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Berinstein, Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Gomez, Evangelina Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina 2019-10-29T15:14:41Z 2019-10-29T15:14:41Z 2019-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0264410X19309909 http://hdl.handle.net/20.500.12123/6231 0264-410X https://doi.org/10.1016/j.vaccine.2019.07.069 eng info:eu-repograntAgreement/INTA/PNBIO/1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Vaccine 37 (36) : 5203-5210 (August 2019)
spellingShingle Infectious Bursal Disease Virus
Maternal Immunity
Hens
Vaccines
Virus Bursitis Infecciosa
Inmunidad Maternal
Gallinas
Vacuna
Lucero, Maria Soledad
Richetta, Matias Daniel
Chimeno Zoth, Silvina Andrea
Jaton, Juan
Pinto, Silvina
Canet, Zulma Edith
Berinstein, Analia
Gomez, Evangelina Raquel
Plant-based vaccine candidate against Infectious bursal disease: an alternative to inactivated vaccines for breeder hens
title Plant-based vaccine candidate against Infectious bursal disease: an alternative to inactivated vaccines for breeder hens
title_full Plant-based vaccine candidate against Infectious bursal disease: an alternative to inactivated vaccines for breeder hens
title_fullStr Plant-based vaccine candidate against Infectious bursal disease: an alternative to inactivated vaccines for breeder hens
title_full_unstemmed Plant-based vaccine candidate against Infectious bursal disease: an alternative to inactivated vaccines for breeder hens
title_short Plant-based vaccine candidate against Infectious bursal disease: an alternative to inactivated vaccines for breeder hens
title_sort plant based vaccine candidate against infectious bursal disease an alternative to inactivated vaccines for breeder hens
topic Infectious Bursal Disease Virus
Maternal Immunity
Hens
Vaccines
Virus Bursitis Infecciosa
Inmunidad Maternal
Gallinas
Vacuna
url https://www.sciencedirect.com/science/article/pii/S0264410X19309909
http://hdl.handle.net/20.500.12123/6231
https://doi.org/10.1016/j.vaccine.2019.07.069
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