Bovine macrophages responses to the infection with virulent and attenuated Leptospira interrogans serovar Pomona
Leptospirosis is a zoonosis, caused by pathogenic spirochetes of the genus Leptospira. Although cattle are usually the maintenance hosts of serovar Hardjo, Pomona is the most frequent serovar circulating in Argentina. The understanding of bovine innate immune response and the virulence of this serov...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0378113519300598 http://hdl.handle.net/20.500.12123/6202 https://doi.org/10.1016/j.vetmic.2019.04.033 |
| _version_ | 1855035633598201856 |
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| author | Nagel, Ariel Gaston Vazquez, Cristina Lourdes Etulain, Julia Blanco, Federico Carlos Gravisaco, María José Gomez, Ricardo Martín Caimi, Karina Cynthia |
| author_browse | Blanco, Federico Carlos Caimi, Karina Cynthia Etulain, Julia Gomez, Ricardo Martín Gravisaco, María José Nagel, Ariel Gaston Vazquez, Cristina Lourdes |
| author_facet | Nagel, Ariel Gaston Vazquez, Cristina Lourdes Etulain, Julia Blanco, Federico Carlos Gravisaco, María José Gomez, Ricardo Martín Caimi, Karina Cynthia |
| author_sort | Nagel, Ariel Gaston |
| collection | INTA Digital |
| description | Leptospirosis is a zoonosis, caused by pathogenic spirochetes of the genus Leptospira. Although cattle are usually the maintenance hosts of serovar Hardjo, Pomona is the most frequent serovar circulating in Argentina. The understanding of bovine innate immune response and the virulence of this serovar is important for future control measures. This work compares infection of bovine macrophages with the virulent L. interrogans sv Pomona strain AKRFB (P1) and its attenuated counterpart (P19). First, we confirmed attenuation in the hamster model. Mortality and lung hemorrhages occurred after P1 inoculation, while the survival rate was 100% in P19-infected animals. Cells infected with both strains showed statistically upregulated gene expression of pro-inflammatory cytokines, IL-1β, IL-6 and TNFα. The level of expression of anti-inflammatory cytokine IL-10 was statistically different between strains. Increased expression of IL-10 was observed only in P1-infected cells. For the first time, we describe macrophages extracellular traps induced by infection of bovine macrophages (bMETs) with both, the virulent and attenuated Leptospira interrogans Pomona strains. P1 was found higher internalized when the phagocytosis was inhibited, suggesting a cell entrance of this strain also by an independent-phagocytosis pathway. Furthermore, P1 was higher colocalized with acidic and late endosomal compartments compared with P19. This data emphasizes the importance to deepen in Leptospira bovine macrophages particular invasion mechanisms and, furthermore, underline the value of studying the main hosts. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6202 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA62022019-10-25T10:41:50Z Bovine macrophages responses to the infection with virulent and attenuated Leptospira interrogans serovar Pomona Nagel, Ariel Gaston Vazquez, Cristina Lourdes Etulain, Julia Blanco, Federico Carlos Gravisaco, María José Gomez, Ricardo Martín Caimi, Karina Cynthia Macrophages Live Vaccines Virulent Pathogens Immune Response Macrofagos Leptospira Vacuna Viva Organismos Patógenos Virulentos Bovinae Respuesta Inmunológica Leptospirosis is a zoonosis, caused by pathogenic spirochetes of the genus Leptospira. Although cattle are usually the maintenance hosts of serovar Hardjo, Pomona is the most frequent serovar circulating in Argentina. The understanding of bovine innate immune response and the virulence of this serovar is important for future control measures. This work compares infection of bovine macrophages with the virulent L. interrogans sv Pomona strain AKRFB (P1) and its attenuated counterpart (P19). First, we confirmed attenuation in the hamster model. Mortality and lung hemorrhages occurred after P1 inoculation, while the survival rate was 100% in P19-infected animals. Cells infected with both strains showed statistically upregulated gene expression of pro-inflammatory cytokines, IL-1β, IL-6 and TNFα. The level of expression of anti-inflammatory cytokine IL-10 was statistically different between strains. Increased expression of IL-10 was observed only in P1-infected cells. For the first time, we describe macrophages extracellular traps induced by infection of bovine macrophages (bMETs) with both, the virulent and attenuated Leptospira interrogans Pomona strains. P1 was found higher internalized when the phagocytosis was inhibited, suggesting a cell entrance of this strain also by an independent-phagocytosis pathway. Furthermore, P1 was higher colocalized with acidic and late endosomal compartments compared with P19. This data emphasizes the importance to deepen in Leptospira bovine macrophages particular invasion mechanisms and, furthermore, underline the value of studying the main hosts. Instituto de Biotecnología Fil: Nagel, Ariel Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Etulain, Julia. Academia Nacional de Medicina. Instituto de Medicina Experimental. Laboratorio de Trombosis Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gomez, Ricardo Martín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-10-25T10:36:42Z 2019-10-25T10:36:42Z 2019-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0378113519300598 http://hdl.handle.net/20.500.12123/6202 0378-1135 https://doi.org/10.1016/j.vetmic.2019.04.033 eng info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Microbiology 233 : 124-132 (Junio 2019) |
| spellingShingle | Macrophages Live Vaccines Virulent Pathogens Immune Response Macrofagos Leptospira Vacuna Viva Organismos Patógenos Virulentos Bovinae Respuesta Inmunológica Nagel, Ariel Gaston Vazquez, Cristina Lourdes Etulain, Julia Blanco, Federico Carlos Gravisaco, María José Gomez, Ricardo Martín Caimi, Karina Cynthia Bovine macrophages responses to the infection with virulent and attenuated Leptospira interrogans serovar Pomona |
| title | Bovine macrophages responses to the infection with virulent and attenuated Leptospira interrogans serovar Pomona |
| title_full | Bovine macrophages responses to the infection with virulent and attenuated Leptospira interrogans serovar Pomona |
| title_fullStr | Bovine macrophages responses to the infection with virulent and attenuated Leptospira interrogans serovar Pomona |
| title_full_unstemmed | Bovine macrophages responses to the infection with virulent and attenuated Leptospira interrogans serovar Pomona |
| title_short | Bovine macrophages responses to the infection with virulent and attenuated Leptospira interrogans serovar Pomona |
| title_sort | bovine macrophages responses to the infection with virulent and attenuated leptospira interrogans serovar pomona |
| topic | Macrophages Live Vaccines Virulent Pathogens Immune Response Macrofagos Leptospira Vacuna Viva Organismos Patógenos Virulentos Bovinae Respuesta Inmunológica |
| url | https://www.sciencedirect.com/science/article/pii/S0378113519300598 http://hdl.handle.net/20.500.12123/6202 https://doi.org/10.1016/j.vetmic.2019.04.033 |
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