Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
Background: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3)...
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| Format: | Artículo |
| Language: | Inglés |
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Wolters Kluwer; Asian African Society of Mycobacteriology
2019
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| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=2;spage=138;epage=145;aulast=Oriani http://hdl.handle.net/20.500.12123/6200 https://doi.org/10.4103/ijmy.ijmy_39_19 |
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| author | Oriani, Alejandra Soledad Marfil, Maria Jimena Zumarraga, Martin Jose Baldini, Mónica Diana |
| author_browse | Baldini, Mónica Diana Marfil, Maria Jimena Oriani, Alejandra Soledad Zumarraga, Martin Jose |
| author_facet | Oriani, Alejandra Soledad Marfil, Maria Jimena Zumarraga, Martin Jose Baldini, Mónica Diana |
| author_sort | Oriani, Alejandra Soledad |
| collection | INTA Digital |
| description | Background: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were combined to detect and identify NTM. Results: NTM were isolated in 51.6% (64/124) of all the samples analyzed. Mycobacterium gordonae was the most frequently isolated organism (15/64) in all samples analyzed, followed by Mycobacterium peregrinum and Mycobacterium frederiksbergense. Significant differences were found in the residual chlorine values between sampling S2 and S3. In both sites, maximum counts were recorded but they did not correlate with low chlorine values. A concentration higher than 500 colony-forming unit/L of NTM was never found, which can be attributed to the negative effect caused by decontamination methods being a point to consider for the recovery of NTM. In 46.9% (30/64) of samples, both methods coincided in the identification, and the obtained sequences presented ≥99% identity. Identification at the species level was achieved in 50% (32/64) of the isolates. Nearly 17.2% (11/64) of the isolates showed a similarity <99%. Conclusions: It should be taken into account that sequencing of the 16S rRNA gene and biochemical tests are useful for the identification of several species, but it is necessary to incorporate other genes (hsp 65 and rpo B) to obtain accurate identification. |
| format | Artículo |
| id | INTA6200 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wolters Kluwer; Asian African Society of Mycobacteriology |
| publisherStr | Wolters Kluwer; Asian African Society of Mycobacteriology |
| record_format | dspace |
| spelling | INTA62002019-10-24T18:21:56Z Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina Oriani, Alejandra Soledad Marfil, Maria Jimena Zumarraga, Martin Jose Baldini, Mónica Diana Drinking Water Systems Isolation Techniques Red de Agua Potable Técnicas de Aislamiento Mycobacterium Identification Methods Nontuberculous Mycobacteria Métodos de identificación Bahía Blanca, Buenos Aires Background: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were combined to detect and identify NTM. Results: NTM were isolated in 51.6% (64/124) of all the samples analyzed. Mycobacterium gordonae was the most frequently isolated organism (15/64) in all samples analyzed, followed by Mycobacterium peregrinum and Mycobacterium frederiksbergense. Significant differences were found in the residual chlorine values between sampling S2 and S3. In both sites, maximum counts were recorded but they did not correlate with low chlorine values. A concentration higher than 500 colony-forming unit/L of NTM was never found, which can be attributed to the negative effect caused by decontamination methods being a point to consider for the recovery of NTM. In 46.9% (30/64) of samples, both methods coincided in the identification, and the obtained sequences presented ≥99% identity. Identification at the species level was achieved in 50% (32/64) of the isolates. Nearly 17.2% (11/64) of the isolates showed a similarity <99%. Conclusions: It should be taken into account that sequencing of the 16S rRNA gene and biochemical tests are useful for the identification of several species, but it is necessary to incorporate other genes (hsp 65 and rpo B) to obtain accurate identification. Instituto de Biotecnología Fil: Oriani, Alejandra Soledad. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Baldini, Mónica Diana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina 2019-10-24T18:06:01Z 2019-10-24T18:06:01Z 2019-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=2;spage=138;epage=145;aulast=Oriani http://hdl.handle.net/20.500.12123/6200 2212-5531 https://doi.org/10.4103/ijmy.ijmy_39_19 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Buenos Aires (province) Wolters Kluwer; Asian African Society of Mycobacteriology International journal of mycobacteriology 8 (2): 138‑145. (14-Jun-2019) |
| spellingShingle | Drinking Water Systems Isolation Techniques Red de Agua Potable Técnicas de Aislamiento Mycobacterium Identification Methods Nontuberculous Mycobacteria Métodos de identificación Bahía Blanca, Buenos Aires Oriani, Alejandra Soledad Marfil, Maria Jimena Zumarraga, Martin Jose Baldini, Mónica Diana Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina |
| title | Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina |
| title_full | Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina |
| title_fullStr | Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina |
| title_full_unstemmed | Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina |
| title_short | Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina |
| title_sort | prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of bahia blanca city argentina |
| topic | Drinking Water Systems Isolation Techniques Red de Agua Potable Técnicas de Aislamiento Mycobacterium Identification Methods Nontuberculous Mycobacteria Métodos de identificación Bahía Blanca, Buenos Aires |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=2;spage=138;epage=145;aulast=Oriani http://hdl.handle.net/20.500.12123/6200 https://doi.org/10.4103/ijmy.ijmy_39_19 |
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