Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work we...
| Autores principales: | , , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/617 http://www.tandfonline.com/doi/abs/10.1080/10495398.2016.1262868?journalCode=labt20 http://dx.doi.org/10.1080/10495398.2016.1262868 |
| _version_ | 1855034677469904896 |
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| author | Goszczynski, Daniel Estanislao Papaleo Mazzucco, Juliana Ripoli, María Verónica Villarreal, Edgardo Leopoldo Rogberg Muñoz, Andres Mezzadra, Carlos Alberto Melucci, Lilia Magdalena Giovambattista, Guillermo |
| author_browse | Giovambattista, Guillermo Goszczynski, Daniel Estanislao Melucci, Lilia Magdalena Mezzadra, Carlos Alberto Papaleo Mazzucco, Juliana Ripoli, María Verónica Rogberg Muñoz, Andres Villarreal, Edgardo Leopoldo |
| author_facet | Goszczynski, Daniel Estanislao Papaleo Mazzucco, Juliana Ripoli, María Verónica Villarreal, Edgardo Leopoldo Rogberg Muñoz, Andres Mezzadra, Carlos Alberto Melucci, Lilia Magdalena Giovambattista, Guillermo |
| author_sort | Goszczynski, Daniel Estanislao |
| collection | INTA Digital |
| description | FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms. Haplotypes and linkage disequilibrium analyses showed a high variability. Five SNPs were selected to perform validation and association studies in our crossbred population. Four SNPs showed well-balanced allele frequencies (minor frequency > 0.159), and three showed no significant deviations from Hardy-Weinberg proportions. SNPs showed significant effects on backfat thickness and fatty acid composition (P < 0.05). The protein structure of one of the missense SNPs was analyzed to elucidate its possible effect on fat content in our studied population. Our results revealed a possible blockage of the fatty acid binding site by the missense mutation. |
| format | info:eu-repo/semantics/article |
| id | INTA617 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA6172024-03-01T12:34:26Z Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content Goszczynski, Daniel Estanislao Papaleo Mazzucco, Juliana Ripoli, María Verónica Villarreal, Edgardo Leopoldo Rogberg Muñoz, Andres Mezzadra, Carlos Alberto Melucci, Lilia Magdalena Giovambattista, Guillermo Variación Genética Genetic Variation Beef Cattle Lipid Content Ganado de Carne Contenido de Lípidos Gen FABP4 FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms. Haplotypes and linkage disequilibrium analyses showed a high variability. Five SNPs were selected to perform validation and association studies in our crossbred population. Four SNPs showed well-balanced allele frequencies (minor frequency > 0.159), and three showed no significant deviations from Hardy-Weinberg proportions. SNPs showed significant effects on backfat thickness and fatty acid composition (P < 0.05). The protein structure of one of the missense SNPs was analyzed to elucidate its possible effect on fat content in our studied population. Our results revealed a possible blockage of the fatty acid binding site by the missense mutation. EEA Balcarce Fil: Goszczynski, Daniel Estanislao. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Papaleo Mazzucco, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Villarreal, Edgardo Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina Fil: Mezzadra, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Melucci, Lilia Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina 2017-07-10T12:30:06Z 2017-07-10T12:30:06Z 2017-01-05 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/617 http://www.tandfonline.com/doi/abs/10.1080/10495398.2016.1262868?journalCode=labt20 Daniel E. Goszczynski, Juliana Papaleo-Mazzucco, María V. Ripoli, Edgardo L. Villarreal, Andrés Rogberg-Muñoz, Carlos A. Mezzadra, Lilia M. Melucci & Guillermo Giovambattista (2017): Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content, Animal Biotechnology, DOI: 10.1080/10495398.2016.1262868 1532-2378 http://dx.doi.org/10.1080/10495398.2016.1262868 eng info:eu-repo/semantics/restrictedAccess application/pdf Animal Biotechnology 28 (3) : 2-9 |
| spellingShingle | Variación Genética Genetic Variation Beef Cattle Lipid Content Ganado de Carne Contenido de Lípidos Gen FABP4 Goszczynski, Daniel Estanislao Papaleo Mazzucco, Juliana Ripoli, María Verónica Villarreal, Edgardo Leopoldo Rogberg Muñoz, Andres Mezzadra, Carlos Alberto Melucci, Lilia Magdalena Giovambattista, Guillermo Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content |
| title | Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content |
| title_full | Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content |
| title_fullStr | Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content |
| title_full_unstemmed | Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content |
| title_short | Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content |
| title_sort | genetic variation in fabp4 and evaluation of its effects on beef cattle fat content |
| topic | Variación Genética Genetic Variation Beef Cattle Lipid Content Ganado de Carne Contenido de Lípidos Gen FABP4 |
| url | http://hdl.handle.net/20.500.12123/617 http://www.tandfonline.com/doi/abs/10.1080/10495398.2016.1262868?journalCode=labt20 http://dx.doi.org/10.1080/10495398.2016.1262868 |
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