Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition
Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overex...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
American Society of Plant Biologists
2019
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| Materias: | |
| Acceso en línea: | http://www.plantphysiol.org/content/158/1/252 http://hdl.handle.net/20.500.12123/6165 https://doi.org/10.1104/pp.111.186544 |
| _version_ | 1855035625589178368 |
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| author | Nahirñak, Vanesa Almasia, Natalia Ines Fernandez, Paula Virginia Hopp, Horacio Esteban Estevez, Jose Manuel Carrari, Fernando Vazquez Rovere, Cecilia |
| author_browse | Almasia, Natalia Ines Carrari, Fernando Estevez, Jose Manuel Fernandez, Paula Virginia Hopp, Horacio Esteban Nahirñak, Vanesa Vazquez Rovere, Cecilia |
| author_facet | Nahirñak, Vanesa Almasia, Natalia Ines Fernandez, Paula Virginia Hopp, Horacio Esteban Estevez, Jose Manuel Carrari, Fernando Vazquez Rovere, Cecilia |
| author_sort | Nahirñak, Vanesa |
| collection | INTA Digital |
| description | Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70% to 90% increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6165 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | American Society of Plant Biologists |
| publisherStr | American Society of Plant Biologists |
| record_format | dspace |
| spelling | INTA61652019-10-22T12:08:06Z Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition Nahirñak, Vanesa Almasia, Natalia Ines Fernandez, Paula Virginia Hopp, Horacio Esteban Estevez, Jose Manuel Carrari, Fernando Vazquez Rovere, Cecilia Papa Péptidos Metabolismo Pared Celular División Celular Antimicrobianos Potatoes Peptides Metabolism Cell Walls Cell Division Antimicrobials Snakin-1 Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70% to 90% increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense. Instituto de Biotecnología Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Fernandez, Paula Virginia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Química de Biomoléculas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2019-10-22T12:05:18Z 2019-10-22T12:05:18Z 2012-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://www.plantphysiol.org/content/158/1/252 http://hdl.handle.net/20.500.12123/6165 0032-0889 1532-2548 https://doi.org/10.1104/pp.111.186544 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Society of Plant Biologists Plant Physiology 158 (1) : 252-263 (January 2012) |
| spellingShingle | Papa Péptidos Metabolismo Pared Celular División Celular Antimicrobianos Potatoes Peptides Metabolism Cell Walls Cell Division Antimicrobials Snakin-1 Nahirñak, Vanesa Almasia, Natalia Ines Fernandez, Paula Virginia Hopp, Horacio Esteban Estevez, Jose Manuel Carrari, Fernando Vazquez Rovere, Cecilia Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition |
| title | Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition |
| title_full | Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition |
| title_fullStr | Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition |
| title_full_unstemmed | Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition |
| title_short | Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition |
| title_sort | potato snakin 1 gene silencing affects cell division primary metabolism and cell wall composition |
| topic | Papa Péptidos Metabolismo Pared Celular División Celular Antimicrobianos Potatoes Peptides Metabolism Cell Walls Cell Division Antimicrobials Snakin-1 |
| url | http://www.plantphysiol.org/content/158/1/252 http://hdl.handle.net/20.500.12123/6165 https://doi.org/10.1104/pp.111.186544 |
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