Redox systemic signaling and induced tolerance responses during soybean–bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation
The symbiotic relationship between legumes and nitrogen-fixing rhizobia induces local and systemic responses, which ultimately lead to nodule formation. The autoregulation of nodulation (AON) is a systemic mechanism related to innate immunity that controls nodule development and involves different c...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Español |
| Publicado: |
Frontiers Media
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6090 https://www.frontiersin.org/articles/10.3389/fpls.2019.00141/full https://doi.org/10.3389/fpls.2019.00141 |
| _version_ | 1855035612307914752 |
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| author | Fernandez Göbel, Tadeo Francisco Deanna, Rocío Muñoz, Nacira Belen Robert, German Asurmendi, Sebastian Lascano, Hernan Ramiro |
| author_browse | Asurmendi, Sebastian Deanna, Rocío Fernandez Göbel, Tadeo Francisco Lascano, Hernan Ramiro Muñoz, Nacira Belen Robert, German |
| author_facet | Fernandez Göbel, Tadeo Francisco Deanna, Rocío Muñoz, Nacira Belen Robert, German Asurmendi, Sebastian Lascano, Hernan Ramiro |
| author_sort | Fernandez Göbel, Tadeo Francisco |
| collection | INTA Digital |
| description | The symbiotic relationship between legumes and nitrogen-fixing rhizobia induces local and systemic responses, which ultimately lead to nodule formation. The autoregulation of nodulation (AON) is a systemic mechanism related to innate immunity that controls nodule development and involves different components ranging from hormones, peptides, receptors to small RNAs. Here, we characterized a rapid systemic redox changes induced during soybean-Bradyrhizobium japonicum symbiotic interaction. A transient peak of reactive oxygen species (ROS) generation was found in soybean leaves after 30 min of root inoculation with B. japonicum. The ROS response was accompanied by changes in the redox state of glutathione and by activation of antioxidant enzymes. Moreover, the ROS peak and antioxidant enzyme activation were abolished in leaves by the addition, in either root or leaf, of DPI, an NADPH oxidase inhibitor. Likewise, these systemic redox changes primed the plant increasing its tolerance to photooxidative stress. With the use of non-nodulating nfr5-mutant and hyper-nodulating nark-mutant soybean plants, we subsequently studied the systemic redox changes. The nfr5-mutant lacked the systemic redox changes after inoculation, whereas the nark-mutant showed a similar redox systemic signaling than the wild type plants. However, neither nfr5- nor nark-mutant exhibited tolerance to photooxidative stress condition. Altogether, these results demonstrated that (i) the early redox systemic signaling during symbiotic interaction depends on a Nod factor receptor, and that (ii) the induced tolerance response depends on the AON mechanisms. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6090 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA60902024-08-01T10:29:29Z Redox systemic signaling and induced tolerance responses during soybean–bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation Fernandez Göbel, Tadeo Francisco Deanna, Rocío Muñoz, Nacira Belen Robert, German Asurmendi, Sebastian Lascano, Hernan Ramiro Rhizobiaceae Rhizobium Simbiosis Nodulación Bradyrhizobium Japonicum Soja Symbiosis Root Nodulation Soybeans Redox Potential Potencial Redox ISR/PGPR The symbiotic relationship between legumes and nitrogen-fixing rhizobia induces local and systemic responses, which ultimately lead to nodule formation. The autoregulation of nodulation (AON) is a systemic mechanism related to innate immunity that controls nodule development and involves different components ranging from hormones, peptides, receptors to small RNAs. Here, we characterized a rapid systemic redox changes induced during soybean-Bradyrhizobium japonicum symbiotic interaction. A transient peak of reactive oxygen species (ROS) generation was found in soybean leaves after 30 min of root inoculation with B. japonicum. The ROS response was accompanied by changes in the redox state of glutathione and by activation of antioxidant enzymes. Moreover, the ROS peak and antioxidant enzyme activation were abolished in leaves by the addition, in either root or leaf, of DPI, an NADPH oxidase inhibitor. Likewise, these systemic redox changes primed the plant increasing its tolerance to photooxidative stress. With the use of non-nodulating nfr5-mutant and hyper-nodulating nark-mutant soybean plants, we subsequently studied the systemic redox changes. The nfr5-mutant lacked the systemic redox changes after inoculation, whereas the nark-mutant showed a similar redox systemic signaling than the wild type plants. However, neither nfr5- nor nark-mutant exhibited tolerance to photooxidative stress condition. Altogether, these results demonstrated that (i) the early redox systemic signaling during symbiotic interaction depends on a Nod factor receptor, and that (ii) the induced tolerance response depends on the AON mechanisms. Instituto de Biotecnología Fil: Fernandez Göbel, Tadeo Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fil: Deanna, Rocío. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina 2019-10-10T15:09:28Z 2019-10-10T15:09:28Z 2019-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6090 https://www.frontiersin.org/articles/10.3389/fpls.2019.00141/full 1664-462X https://doi.org/10.3389/fpls.2019.00141 spa info:eu-repograntAgreement/INTA/PNBIO/1131022/AR./Genómica funcional y biología de sistemas. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in plant science 10: 141 (2019 Feb 15) |
| spellingShingle | Rhizobiaceae Rhizobium Simbiosis Nodulación Bradyrhizobium Japonicum Soja Symbiosis Root Nodulation Soybeans Redox Potential Potencial Redox ISR/PGPR Fernandez Göbel, Tadeo Francisco Deanna, Rocío Muñoz, Nacira Belen Robert, German Asurmendi, Sebastian Lascano, Hernan Ramiro Redox systemic signaling and induced tolerance responses during soybean–bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation |
| title | Redox systemic signaling and induced tolerance responses during soybean–bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation |
| title_full | Redox systemic signaling and induced tolerance responses during soybean–bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation |
| title_fullStr | Redox systemic signaling and induced tolerance responses during soybean–bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation |
| title_full_unstemmed | Redox systemic signaling and induced tolerance responses during soybean–bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation |
| title_short | Redox systemic signaling and induced tolerance responses during soybean–bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation |
| title_sort | redox systemic signaling and induced tolerance responses during soybean bradyrhizobium japonicum interaction involvement of nod factor receptor and autoregulation of nodulation |
| topic | Rhizobiaceae Rhizobium Simbiosis Nodulación Bradyrhizobium Japonicum Soja Symbiosis Root Nodulation Soybeans Redox Potential Potencial Redox ISR/PGPR |
| url | http://hdl.handle.net/20.500.12123/6090 https://www.frontiersin.org/articles/10.3389/fpls.2019.00141/full https://doi.org/10.3389/fpls.2019.00141 |
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