Studies on the essential YNL152w open reading frame in Saccharomyces cerevisiae
Tesis para obtener el grado de Doctor en Biología/Química de la Universität Osnabrück, en 2007
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| Formato: | Tesis |
| Lenguaje: | Inglés |
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Osnabrück Universität, Alemania
2019
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/6081 https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007062910 |
| _version_ | 1855483742152294400 |
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| author | Ciklic, Ivan Francisco |
| author2 | Heinisch, Jürgen (director) |
| author_browse | Ciklic, Ivan Francisco Heinisch, Jürgen (director) |
| author_facet | Heinisch, Jürgen (director) Ciklic, Ivan Francisco |
| author_sort | Ciklic, Ivan Francisco |
| collection | INTA Digital |
| description | Tesis para obtener el grado de Doctor en Biología/Química de la Universität Osnabrück, en 2007 |
| format | Tesis |
| id | INTA6081 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Osnabrück Universität, Alemania |
| publisherStr | Osnabrück Universität, Alemania |
| record_format | dspace |
| spelling | INTA60812021-08-25T18:02:28Z Studies on the essential YNL152w open reading frame in Saccharomyces cerevisiae Ciklic, Ivan Francisco Heinisch, Jürgen (director) Saccharomyces Cerevisiae Genética División Celular Citoquininas Fenotipos Genetics Cell Division Cytokinins Phenotypes Gen YNL152w Gene YNL152w Tesis para obtener el grado de Doctor en Biología/Química de la Universität Osnabrück, en 2007 The essential gene YNL152w was previously found in a screen designed to isolate putative negative regulators of the S. cerevisiae Pkc1p pathway. Activity assays were performed with a lexA-RLM1-lacZ integrated reporter in different ynl152w truncated mutants. In contrast to the original screen, there were no differences or the activities were even lower in some mutants. To analyze the consequences of different expression levels, YNL152w was expressed under the control of the GAL1/10 promoter. Growth curves were performed under high, intermediate and low expression levels. Strikingly, both conditional strains were able to grow under repressing conditions. However, an aberrant morphology was found suggesting that the cells are indeed affected by low amounts of Ynl152w protein. A series of successive Ynl152wp C-terminal truncations was analyzed to determine cell viability and to investigate the function of the protein. Remarkably, about 2/3 of the protein were dispensable to confer viability. Microscopic analyses of constructs revealed an aberrant morphology characteristic of a cytokinesis defective mutant, with the appearance of swollen cells and formation of big aggregates. Interestingly, the phenotype was more pronounced in the larger truncations. Coherent with these results time-lapse experiments with a large truncated mutant showed a stabilization of the SH3 protein Hof1p at the bud neck. This protein is involved in septum formation and has been reported as a binding partner of YNL152w. The phenotypes observed in the truncated mutants could be attributed to the presence of 4 proline rich motifs. Such motifs have been reported to interact with SH3 domains. An internal deletion of an aspartate rich domain present in the Ynl152wp sequence also displayed a phenotype very similar to that of the largest truncations. Therefore, this domain may play an important role in Ynl152wp function. EEA Mendoza Fil: Ciklic, Ivan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina 2019-10-10T12:29:37Z 2019-10-10T12:29:37Z 2007 info:ar-repo/semantics/tesis doctoral info:eu-repo/semantics/doctoralThesis info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6081 https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007062910 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Osnabrück Universität, Alemania |
| spellingShingle | Saccharomyces Cerevisiae Genética División Celular Citoquininas Fenotipos Genetics Cell Division Cytokinins Phenotypes Gen YNL152w Gene YNL152w Ciklic, Ivan Francisco Studies on the essential YNL152w open reading frame in Saccharomyces cerevisiae |
| title | Studies on the essential YNL152w open reading frame in Saccharomyces cerevisiae |
| title_full | Studies on the essential YNL152w open reading frame in Saccharomyces cerevisiae |
| title_fullStr | Studies on the essential YNL152w open reading frame in Saccharomyces cerevisiae |
| title_full_unstemmed | Studies on the essential YNL152w open reading frame in Saccharomyces cerevisiae |
| title_short | Studies on the essential YNL152w open reading frame in Saccharomyces cerevisiae |
| title_sort | studies on the essential ynl152w open reading frame in saccharomyces cerevisiae |
| topic | Saccharomyces Cerevisiae Genética División Celular Citoquininas Fenotipos Genetics Cell Division Cytokinins Phenotypes Gen YNL152w Gene YNL152w |
| url | http://hdl.handle.net/20.500.12123/6081 https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007062910 |
| work_keys_str_mv | AT ciklicivanfrancisco studiesontheessentialynl152wopenreadingframeinsaccharomycescerevisiae |