Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resis...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Pontificia Universidad Católica de Valparaíso
2019
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/6031 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v14n3-14 https://doi.org/10.2225/vol14-issue3-fulltext-14 |
| _version_ | 1855035602002509824 |
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| author | Vanzetti, Leonardo Sebastian Campos, Pablo Eduardo Demichelis, Melina Lombardo, Lucio Aurelia, Paola Romina Vaschetto, Luis María Bainotti, Carlos Tomas Helguera, Marcelo |
| author_browse | Aurelia, Paola Romina Bainotti, Carlos Tomas Campos, Pablo Eduardo Demichelis, Melina Helguera, Marcelo Lombardo, Lucio Vanzetti, Leonardo Sebastian Vaschetto, Luis María |
| author_facet | Vanzetti, Leonardo Sebastian Campos, Pablo Eduardo Demichelis, Melina Lombardo, Lucio Aurelia, Paola Romina Vaschetto, Luis María Bainotti, Carlos Tomas Helguera, Marcelo |
| author_sort | Vanzetti, Leonardo Sebastian |
| collection | INTA Digital |
| description | Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four differential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust infection types produced on seedling plants of the 66 local cultivars were compared with the infection types produced by the same pathotypes on Lr differentials to postulate which seedling leaf rust genes were present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using molecular markers. Eleven different Lr genes were postulated in the material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26, Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined with the seventeen pathotypes used in the study because all were avirulent to these genes. Eleven cultivars (16.7%) were resistant to all pathotypes used in the study and the remaining 55 (83.3%) showed virulent reaction against one or more local pathotypes. Cultivars with seedling resistance gene combinations including Lr16 or single genes Lr47 (detected with molecular marker), Lr19 and Lr41, showed high levels of resistance against all pathotypes or most of them. On the opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a + Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number of virulent reactions against local pathotypes. Occurrence of adult plant resistance genes Lr34, Lr35 and Lr37 in local germplasm was evaluated using specific molecular markers confirming presence of Lr34 and Lr37. Our data suggest that combinations including seedling resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with adult plant resistance genes like Lr34, SV2, Lr46 will probably provide durable and effective resistance to leaf rust in the region. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6031 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Pontificia Universidad Católica de Valparaíso |
| publisherStr | Pontificia Universidad Católica de Valparaíso |
| record_format | dspace |
| spelling | INTA60312019-10-01T15:14:54Z Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers Vanzetti, Leonardo Sebastian Campos, Pablo Eduardo Demichelis, Melina Lombardo, Lucio Aurelia, Paola Romina Vaschetto, Luis María Bainotti, Carlos Tomas Helguera, Marcelo Marcadores Genéticos Fitomejoramiento Trigo Harinero Resistencia a la Enfermedad Roya Genes Genetic Markers Plant Breeding Soft Wheat Disease Resistance Rusts Argentina Marcadores Moleculares Molecular Markers Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four differential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust infection types produced on seedling plants of the 66 local cultivars were compared with the infection types produced by the same pathotypes on Lr differentials to postulate which seedling leaf rust genes were present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using molecular markers. Eleven different Lr genes were postulated in the material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26, Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined with the seventeen pathotypes used in the study because all were avirulent to these genes. Eleven cultivars (16.7%) were resistant to all pathotypes used in the study and the remaining 55 (83.3%) showed virulent reaction against one or more local pathotypes. Cultivars with seedling resistance gene combinations including Lr16 or single genes Lr47 (detected with molecular marker), Lr19 and Lr41, showed high levels of resistance against all pathotypes or most of them. On the opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a + Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number of virulent reactions against local pathotypes. Occurrence of adult plant resistance genes Lr34, Lr35 and Lr37 in local germplasm was evaluated using specific molecular markers confirming presence of Lr34 and Lr37. Our data suggest that combinations including seedling resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with adult plant resistance genes like Lr34, SV2, Lr46 will probably provide durable and effective resistance to leaf rust in the region. EEA Marcos Juárez Fil: Vanzetti, Leonardo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina Fil: Campos, Pablo Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave. Area Fitopatología; Argentina Fil: Demichelis, Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina Fil: Lombardo, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina Fil: Aurelia, Paola Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina Fil: Vaschetto, Luis María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina Fil: Bainotti, Carlos Tomas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Mejoramiento de Trigo; Argentina Fil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina 2019-10-01T15:08:13Z 2019-10-01T15:08:13Z 2011 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6031 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v14n3-14 0717-3458 https://doi.org/10.2225/vol14-issue3-fulltext-14 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Pontificia Universidad Católica de Valparaíso Electronic journal of biotechnology 14 (3) : 1-17. (2011) |
| spellingShingle | Marcadores Genéticos Fitomejoramiento Trigo Harinero Resistencia a la Enfermedad Roya Genes Genetic Markers Plant Breeding Soft Wheat Disease Resistance Rusts Argentina Marcadores Moleculares Molecular Markers Vanzetti, Leonardo Sebastian Campos, Pablo Eduardo Demichelis, Melina Lombardo, Lucio Aurelia, Paola Romina Vaschetto, Luis María Bainotti, Carlos Tomas Helguera, Marcelo Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers |
| title | Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers |
| title_full | Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers |
| title_fullStr | Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers |
| title_full_unstemmed | Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers |
| title_short | Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers |
| title_sort | identification of leaf rust resistance genes in selected argentinean bread wheat cultivars by gene postulation and molecular markers |
| topic | Marcadores Genéticos Fitomejoramiento Trigo Harinero Resistencia a la Enfermedad Roya Genes Genetic Markers Plant Breeding Soft Wheat Disease Resistance Rusts Argentina Marcadores Moleculares Molecular Markers |
| url | http://hdl.handle.net/20.500.12123/6031 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v14n3-14 https://doi.org/10.2225/vol14-issue3-fulltext-14 |
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