Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
Bovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Wiley
2019
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| Materias: | |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.x http://hdl.handle.net/20.500.12123/6030 https://doi.org/10.1111/j.1439-0531.2010.01668.x |
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| author | Morrell, Eleonora Lidia Barbeito, Claudio Gustavo Odeon, Anselmo Carlos Gimeno, Eduardo Juan Campero, Carlos Manuel |
| author_browse | Barbeito, Claudio Gustavo Campero, Carlos Manuel Gimeno, Eduardo Juan Morrell, Eleonora Lidia Odeon, Anselmo Carlos |
| author_facet | Morrell, Eleonora Lidia Barbeito, Claudio Gustavo Odeon, Anselmo Carlos Gimeno, Eduardo Juan Campero, Carlos Manuel |
| author_sort | Morrell, Eleonora Lidia |
| collection | INTA Digital |
| description | Bovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was neutrophilic bronchopneumonia and interstitial pneumonia. Other commonly observed lesions included non‐suppurative interstitial enteritis, hepatitis, pericarditis, myositis, myocarditis, and meningitis. In this study, C. fetus fetus was phenotypically classified in all bovine foetuses from lungs and abomasal fluids. Immunohistochemistry (IHC) staining revealed positive stained Campylobacter organisms with typical morphology. Lectin binding patterns not showed great differences between the infected and the non‐infected groups. The most important changes were a minor peanut agglutinin (PNA) and DBA binding in the alveolar cells of the lungs and DBA globet cells in some of the C. fetus–positive foetuses. Individual variations in each lectin binding pattern complicate the evaluation of the lectins results. All foetuses positive to IHC were positive by PCR. Better efficiency of PCR was obtained from abomasal fluids than from lung tissues. The association of culture and phenotypic techniques with histopathology, IHC and PCR allowed a better characterization and description of BC. |
| format | Artículo |
| id | INTA6030 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA60302019-10-01T14:38:53Z Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus Morrell, Eleonora Lidia Barbeito, Claudio Gustavo Odeon, Anselmo Carlos Gimeno, Eduardo Juan Campero, Carlos Manuel Ganado Bovino Enfermedades de los Animales Campylobacter fetus Aborto Histopatología Inmunohistoquímica PCR Diagnóstico Cattle Animal Diseases Abortion Histopathology Immunohistochemistry Diagnosis Reacción en Cadena de la Polimerasa Polymerase Chain Reaction Bovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was neutrophilic bronchopneumonia and interstitial pneumonia. Other commonly observed lesions included non‐suppurative interstitial enteritis, hepatitis, pericarditis, myositis, myocarditis, and meningitis. In this study, C. fetus fetus was phenotypically classified in all bovine foetuses from lungs and abomasal fluids. Immunohistochemistry (IHC) staining revealed positive stained Campylobacter organisms with typical morphology. Lectin binding patterns not showed great differences between the infected and the non‐infected groups. The most important changes were a minor peanut agglutinin (PNA) and DBA binding in the alveolar cells of the lungs and DBA globet cells in some of the C. fetus–positive foetuses. Individual variations in each lectin binding pattern complicate the evaluation of the lectins results. All foetuses positive to IHC were positive by PCR. Better efficiency of PCR was obtained from abomasal fluids than from lung tissues. The association of culture and phenotypic techniques with histopathology, IHC and PCR allowed a better characterization and description of BC. EEA Balcarce Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentina Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2019-10-01T14:33:38Z 2019-10-01T14:33:38Z 2011-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.x http://hdl.handle.net/20.500.12123/6030 0936-6768 1439-0531 https://doi.org/10.1111/j.1439-0531.2010.01668.x eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Reproduction in Domestic Animals 46 (2) : 309-315 (April 2011) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Campylobacter fetus Aborto Histopatología Inmunohistoquímica PCR Diagnóstico Cattle Animal Diseases Abortion Histopathology Immunohistochemistry Diagnosis Reacción en Cadena de la Polimerasa Polymerase Chain Reaction Morrell, Eleonora Lidia Barbeito, Claudio Gustavo Odeon, Anselmo Carlos Gimeno, Eduardo Juan Campero, Carlos Manuel Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus |
| title | Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus |
| title_full | Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus |
| title_fullStr | Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus |
| title_full_unstemmed | Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus |
| title_short | Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus |
| title_sort | histopathological immunohistochemical lectinhistochemical and molecular findings in spontaneous bovine abortions by campylobacter fetus |
| topic | Ganado Bovino Enfermedades de los Animales Campylobacter fetus Aborto Histopatología Inmunohistoquímica PCR Diagnóstico Cattle Animal Diseases Abortion Histopathology Immunohistochemistry Diagnosis Reacción en Cadena de la Polimerasa Polymerase Chain Reaction |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.x http://hdl.handle.net/20.500.12123/6030 https://doi.org/10.1111/j.1439-0531.2010.01668.x |
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