A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0021997518302342 http://hdl.handle.net/20.500.12123/6021 https://doi.org/10.1016/j.jcpa.2018.10.172 |
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| author | Dorsch, Matías A. de Yaniz, María Guadalupe Fiorani, Franco Hecker, Yanina Paola Odeon, Anselmo Carlos Morrell, Eleonora Lidia Campero, Carlos Manuel Barbeito, Claudio Gustavo Moore, Prando Dadin |
| author_browse | Barbeito, Claudio Gustavo Campero, Carlos Manuel Dorsch, Matías A. Fiorani, Franco Hecker, Yanina Paola Moore, Prando Dadin Morrell, Eleonora Lidia Odeon, Anselmo Carlos de Yaniz, María Guadalupe |
| author_facet | Dorsch, Matías A. de Yaniz, María Guadalupe Fiorani, Franco Hecker, Yanina Paola Odeon, Anselmo Carlos Morrell, Eleonora Lidia Campero, Carlos Manuel Barbeito, Claudio Gustavo Moore, Prando Dadin |
| author_sort | Dorsch, Matías A. |
| collection | INTA Digital |
| description | The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface. |
| format | Artículo |
| id | INTA6021 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA60212019-10-01T14:21:53Z A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. Dorsch, Matías A. de Yaniz, María Guadalupe Fiorani, Franco Hecker, Yanina Paola Odeon, Anselmo Carlos Morrell, Eleonora Lidia Campero, Carlos Manuel Barbeito, Claudio Gustavo Moore, Prando Dadin Ganado Bovino Lectinas Neospora Caninum Placenta Cattle Lectins The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface. EEA Balcarce Fil: Dorsch, Matias A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: de Yañiz, María Guadalupe. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2019-09-30T14:34:34Z 2019-09-30T14:34:34Z 2019-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0021997518302342 http://hdl.handle.net/20.500.12123/6021 0021-9975 https://doi.org/10.1016/j.jcpa.2018.10.172 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Comparative Pathology 166 : 45-53 (January 2019) |
| spellingShingle | Ganado Bovino Lectinas Neospora Caninum Placenta Cattle Lectins Dorsch, Matías A. de Yaniz, María Guadalupe Fiorani, Franco Hecker, Yanina Paola Odeon, Anselmo Carlos Morrell, Eleonora Lidia Campero, Carlos Manuel Barbeito, Claudio Gustavo Moore, Prando Dadin A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
| title | A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
| title_full | A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
| title_fullStr | A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
| title_full_unstemmed | A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
| title_short | A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
| title_sort | descriptive study of lectin histochemistry of the placenta in cattle following inoculation of neospora caninum |
| topic | Ganado Bovino Lectinas Neospora Caninum Placenta Cattle Lectins |
| url | https://www.sciencedirect.com/science/article/pii/S0021997518302342 http://hdl.handle.net/20.500.12123/6021 https://doi.org/10.1016/j.jcpa.2018.10.172 |
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