An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans

Background: The horn fly (Haematobia irritans) is an obligate blood feeder that causes considerable economic losses in livestock industries worldwide. The control of this cattle pest is mainly based on insecticides; unfortunately, in many regions, horn flies have developed resistance. Vaccines or b...

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Autores principales: Ribeiro, J.M., Debat, Humberto Julio, Boiani, M., Ures, X., Rocha, S., Breijo, M.
Formato: Artículo
Lenguaje:Inglés
Publicado: BMC 2019
Materias:
Acceso en línea:https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5984-7
http://hdl.handle.net/20.500.12123/5978
https://doi.org/10.1186/s12864-019-5984-7
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author Ribeiro, J.M.
Debat, Humberto Julio
Boiani, M.
Ures, X.
Rocha, S.
Breijo, M.
author_browse Boiani, M.
Breijo, M.
Debat, Humberto Julio
Ribeiro, J.M.
Rocha, S.
Ures, X.
author_facet Ribeiro, J.M.
Debat, Humberto Julio
Boiani, M.
Ures, X.
Rocha, S.
Breijo, M.
author_sort Ribeiro, J.M.
collection INTA Digital
description Background: The horn fly (Haematobia irritans) is an obligate blood feeder that causes considerable economic losses in livestock industries worldwide. The control of this cattle pest is mainly based on insecticides; unfortunately, in many regions, horn flies have developed resistance. Vaccines or biological control have been proposed as alternative control methods, but the available information about the biology or physiology of this parasite is rather scarce. Results: We present a comprehensive description of the salivary and midgut transcriptomes of the horn fly (Haematobia irritans), using deep sequencing achieved by the Illumina protocol, as well as exploring the virome of this fly. Comparison of the two transcriptomes allow for identification of uniquely salivary or uniquely midgut transcripts, as identified by statistically differential transcript expression at a level of 16 x or more. In addition, we provide genomic highlights and phylogenetic insights of Haematobia irritans Nora virus and present evidence of a novel densovirus, both associated to midgut libraries of H. irritans.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2019
publishDateRange 2019
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spelling INTA59782019-09-26T10:58:32Z An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans Ribeiro, J.M. Debat, Humberto Julio Boiani, M. Ures, X. Rocha, S. Breijo, M. Malaria Haematobia Irritans Viroses Salivary Glands Pests of Animals Plagas de Animales Glándulas Salivales Virosis Vector Biology Horn Fly Midgut Transcriptome Mosca de los Cuernos Background: The horn fly (Haematobia irritans) is an obligate blood feeder that causes considerable economic losses in livestock industries worldwide. The control of this cattle pest is mainly based on insecticides; unfortunately, in many regions, horn flies have developed resistance. Vaccines or biological control have been proposed as alternative control methods, but the available information about the biology or physiology of this parasite is rather scarce. Results: We present a comprehensive description of the salivary and midgut transcriptomes of the horn fly (Haematobia irritans), using deep sequencing achieved by the Illumina protocol, as well as exploring the virome of this fly. Comparison of the two transcriptomes allow for identification of uniquely salivary or uniquely midgut transcripts, as identified by statistically differential transcript expression at a level of 16 x or more. In addition, we provide genomic highlights and phylogenetic insights of Haematobia irritans Nora virus and present evidence of a novel densovirus, both associated to midgut libraries of H. irritans. Instituto de Patología Vegetal Fil: Ribeiro, J.M. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Section of Vector Biology; Estados Unidos Fil: Debat, Humberto J. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Boiani, M. Universidad de la República. Facultad de Medicina. Unidad de Reactivos y Biomodelos de Experimentación; Uruguay Fil: Ures, X. Universidad de la República. Facultad de Medicina. Unidad de Reactivos y Biomodelos de Experimentación; Uruguay Fil: Rocha, S. Universidad de la República. Facultad de Medicina. Unidad de Reactivos y Biomodelos de Experimentación; Uruguay Fil: Breijo, M. Universidad de la República. Facultad de Medicina. Unidad de Reactivos y Biomodelos de Experimentación; Uruguay 2019-09-26T10:47:03Z 2019-09-26T10:47:03Z 2019-07-29 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5984-7 http://hdl.handle.net/20.500.12123/5978 1471-2164 https://doi.org/10.1186/s12864-019-5984-7 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC BMC Genomics 20 (1) : 616 ( July 2019)
spellingShingle Malaria
Haematobia Irritans
Viroses
Salivary Glands
Pests of Animals
Plagas de Animales
Glándulas Salivales
Virosis
Vector Biology
Horn Fly
Midgut
Transcriptome
Mosca de los Cuernos
Ribeiro, J.M.
Debat, Humberto Julio
Boiani, M.
Ures, X.
Rocha, S.
Breijo, M.
An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans
title An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans
title_full An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans
title_fullStr An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans
title_full_unstemmed An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans
title_short An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans
title_sort insight into the sialome mialome and virome of the horn fly haematobia irritans
topic Malaria
Haematobia Irritans
Viroses
Salivary Glands
Pests of Animals
Plagas de Animales
Glándulas Salivales
Virosis
Vector Biology
Horn Fly
Midgut
Transcriptome
Mosca de los Cuernos
url https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5984-7
http://hdl.handle.net/20.500.12123/5978
https://doi.org/10.1186/s12864-019-5984-7
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