Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae

Fungi constituting the Entomophthora muscae species complex (members of subphylum Entomophthoromycotina, phylum Zoopagamycota) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public transcriptome data to identify and characterize eight new...

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Autores principales: Nibert, Max L., Debat, Humberto Julio, Manny, Austin R., Grigoriev, Igor V., De Fine Licht, Henrik H.
Formato: Artículo
Lenguaje:Inglés
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.mdpi.com/1999-4915/11/4/351
http://hdl.handle.net/20.500.12123/5964
https://doi.org/10.3390/v11040351
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author Nibert, Max L.
Debat, Humberto Julio
Manny, Austin R.
Grigoriev, Igor V.
De Fine Licht, Henrik H.
author_browse De Fine Licht, Henrik H.
Debat, Humberto Julio
Grigoriev, Igor V.
Manny, Austin R.
Nibert, Max L.
author_facet Nibert, Max L.
Debat, Humberto Julio
Manny, Austin R.
Grigoriev, Igor V.
De Fine Licht, Henrik H.
author_sort Nibert, Max L.
collection INTA Digital
description Fungi constituting the Entomophthora muscae species complex (members of subphylum Entomophthoromycotina, phylum Zoopagamycota) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public transcriptome data to identify and characterize eight new species of mitoviruses associated with several different E. muscae isolates. Mitoviruses are simple RNA viruses that replicate in host mitochondria and are frequently found in more phylogenetically apical fungi (members of subphylum Glomeromyoctina, phylum Mucoromycota, phylum Basidiomycota and phylum Ascomycota) as well as in plants. E. muscae is the first fungus from phylum Zoopagomycota, and thereby the most phylogenetically basal fungus, found to harbor mitoviruses to date. Multiple UGA (Trp) codons are found not only in each of the new mitovirus sequences from E. muscae but also in mitochondrial core-gene coding sequences newly assembled from E. muscae transcriptome data, suggesting that UGA (Trp) is not a rarely used codon in the mitochondria of this fungus. The presence of mitoviruses in these basal fungi has possible implications for the evolution of these viruses.
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spelling INTA59642019-09-25T11:10:15Z Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae Nibert, Max L. Debat, Humberto Julio Manny, Austin R. Grigoriev, Igor V. De Fine Licht, Henrik H. Entomophthora Fungi Data Mining Minería de Datos Hongos Database Mining Fungal Virus Mitochondrion Mycovirus Virus Discovery Entomophthoromycotina Mitovirus Narnaviridae Fungi constituting the Entomophthora muscae species complex (members of subphylum Entomophthoromycotina, phylum Zoopagamycota) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public transcriptome data to identify and characterize eight new species of mitoviruses associated with several different E. muscae isolates. Mitoviruses are simple RNA viruses that replicate in host mitochondria and are frequently found in more phylogenetically apical fungi (members of subphylum Glomeromyoctina, phylum Mucoromycota, phylum Basidiomycota and phylum Ascomycota) as well as in plants. E. muscae is the first fungus from phylum Zoopagomycota, and thereby the most phylogenetically basal fungus, found to harbor mitoviruses to date. Multiple UGA (Trp) codons are found not only in each of the new mitovirus sequences from E. muscae but also in mitochondrial core-gene coding sequences newly assembled from E. muscae transcriptome data, suggesting that UGA (Trp) is not a rarely used codon in the mitochondria of this fungus. The presence of mitoviruses in these basal fungi has possible implications for the evolution of these viruses. Instituto de Patología Vegetal Fil: Nibert, Max L. Harvard Medical School. Department of Microbiology. Program in Virology; Estados Unidos Fil:Debat, Humberto J. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Manny, Austin R. Harvard Medical School. Department of Microbiology. Program in Virology; Estados Unidos Fil: Grigoriev, , Igor V. U.S. Department of Energy. Joint Genome Institute; Estados Unidos. University of California Berkeley. Department of Plant and Microbial Biology; Estados Unidos Fil: De Fine Licht, Henrik H. University of Copenhagen. Department of Plant and Environmental Sciences; Dinamarca 2019-09-25T10:52:29Z 2019-09-25T10:52:29Z 2019-04-17 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.mdpi.com/1999-4915/11/4/351 http://hdl.handle.net/20.500.12123/5964 1999-4915 https://doi.org/10.3390/v11040351 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 11 (4) : 351. (April 2019)
spellingShingle Entomophthora
Fungi
Data Mining
Minería de Datos
Hongos
Database Mining
Fungal Virus
Mitochondrion
Mycovirus
Virus Discovery
Entomophthoromycotina
Mitovirus
Narnaviridae
Nibert, Max L.
Debat, Humberto Julio
Manny, Austin R.
Grigoriev, Igor V.
De Fine Licht, Henrik H.
Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae
title Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae
title_full Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae
title_fullStr Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae
title_full_unstemmed Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae
title_short Mitovirus and Mitochondrial Coding Sequences from Basal Fungus Entomophthora muscae
title_sort mitovirus and mitochondrial coding sequences from basal fungus entomophthora muscae
topic Entomophthora
Fungi
Data Mining
Minería de Datos
Hongos
Database Mining
Fungal Virus
Mitochondrion
Mycovirus
Virus Discovery
Entomophthoromycotina
Mitovirus
Narnaviridae
url https://www.mdpi.com/1999-4915/11/4/351
http://hdl.handle.net/20.500.12123/5964
https://doi.org/10.3390/v11040351
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