Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
Cyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0960852406005281 http://hdl.handle.net/20.500.12123/5951 https://doi.org/10.1016/j.biortech.2006.09.056 |
| _version_ | 1855483716794580992 |
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| author | Szerman, Natalia Schroh, Ignacio Rossi, Ana Lía Rosso, Adriana Mabel Krymkiewicz, Norberto Ferrarotti, Susana Alicia |
| author_browse | Ferrarotti, Susana Alicia Krymkiewicz, Norberto Rossi, Ana Lía Rosso, Adriana Mabel Schroh, Ignacio Szerman, Natalia |
| author_facet | Szerman, Natalia Schroh, Ignacio Rossi, Ana Lía Rosso, Adriana Mabel Krymkiewicz, Norberto Ferrarotti, Susana Alicia |
| author_sort | Szerman, Natalia |
| collection | INTA Digital |
| description | Cyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, using the same cassava starch as enzyme adsorbent and as substrate. Heat-treatment of raw starch at 70 C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation. The optimum conditions for CD production were: 5% (w/v) cassava starch, 15 U of enzyme per gram of substrate, reaction temperature of 56 C and pH 6.4. After 4 h, the proportion of starch converted to CD reached 66% (w/w) and the weight ratio of a-CD:b-CD:c-CD was 1.00:0.70:0.16. |
| format | Artículo |
| id | INTA5951 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA59512019-09-24T11:26:05Z Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R Szerman, Natalia Schroh, Ignacio Rossi, Ana Lía Rosso, Adriana Mabel Krymkiewicz, Norberto Ferrarotti, Susana Alicia Cyclodextrins Ciclodextrinas Bacillus circulans Cyclodextrins Production Cyclodextrin glycosyltransferase Producción de ciclodextrinas Bacillus circulans DF 9R Cyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, using the same cassava starch as enzyme adsorbent and as substrate. Heat-treatment of raw starch at 70 C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation. The optimum conditions for CD production were: 5% (w/v) cassava starch, 15 U of enzyme per gram of substrate, reaction temperature of 56 C and pH 6.4. After 4 h, the proportion of starch converted to CD reached 66% (w/w) and the weight ratio of a-CD:b-CD:c-CD was 1.00:0.70:0.16. Fil: Szerman, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Schroh, Ignacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Rossi, Ana Lía. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Rosso, Adriana Mabel. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Krymkiewicz, Norberto. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. 2019-09-24T11:20:16Z 2019-09-24T11:20:16Z 2006-09-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0960852406005281 http://hdl.handle.net/20.500.12123/5951 0960-8524 https://doi.org/10.1016/j.biortech.2006.09.056 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Bioresource Technology 98 (15) : 2886–2891 (November 2007). |
| spellingShingle | Cyclodextrins Ciclodextrinas Bacillus circulans Cyclodextrins Production Cyclodextrin glycosyltransferase Producción de ciclodextrinas Bacillus circulans DF 9R Szerman, Natalia Schroh, Ignacio Rossi, Ana Lía Rosso, Adriana Mabel Krymkiewicz, Norberto Ferrarotti, Susana Alicia Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R |
| title | Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R |
| title_full | Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R |
| title_fullStr | Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R |
| title_full_unstemmed | Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R |
| title_short | Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R |
| title_sort | cyclodextrin production by cyclodextrin glycosyltransferase from bacillus circulans df 9r |
| topic | Cyclodextrins Ciclodextrinas Bacillus circulans Cyclodextrins Production Cyclodextrin glycosyltransferase Producción de ciclodextrinas Bacillus circulans DF 9R |
| url | https://www.sciencedirect.com/science/article/pii/S0960852406005281 http://hdl.handle.net/20.500.12123/5951 https://doi.org/10.1016/j.biortech.2006.09.056 |
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