Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina
Fungi require special substrates for their isolation, vegetative growth and sporulation. In experiments conducted in the laboratory, the influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina conidia, the causal agent of soybean frogeye leaf spot, was assesse...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Grupo Paulista de Fitopatologia
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/5912 http://www.scielo.br/pdf/sp/v39n2/08.pdf http://dx.doi.org/10.1590/S0100-54052013000200008 |
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| author | Gomez, Diana Erica Reis, Erlei Melo |
| author_browse | Gomez, Diana Erica Reis, Erlei Melo |
| author_facet | Gomez, Diana Erica Reis, Erlei Melo |
| author_sort | Gomez, Diana Erica |
| collection | INTA Digital |
| description | Fungi require special substrates for their isolation, vegetative
growth and sporulation. In experiments conducted in the laboratory,
the influence of substrates, light, filter paper and pH on the
sporulation of Cercospora sojina conidia, the causal agent of soybean
frogeye leaf spot, was assessed. The media potato sucrose agar, V-8
agar, tomato extract agar, soybean leaf extract agar, soybean seed
extract agar, soybean meal agar, soybean flour agar and wheat flour
agar were tested, added on the surface, with and without filter paper
and under two light regimes, with 12 h light at 25°± 2°C and in the
dark. A triple factorial 8x2x2 (substrates x light/dark x with/without
filter paper) design with four replicates was used. V-8 agar medium
was employed and the pH was adjusted with HCl 0.1N or NaOH 0.1N
before autoclaving to the values: 3, 4, 5, 6, 7 and 8, and the pH of V-8
agar medium is 6.7. The evaluation was done on the seventh day of
incubation. Data underwent regression analysis. Sporulation was
maximized on the agar media V-8, seed extract, oat flour, tomato extract,
and potato sucrose in the presence of filter paper and 12h light. On V-8
medium, maximal sporulation was obtained with pH 6.7. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5912 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Grupo Paulista de Fitopatologia |
| publisherStr | Grupo Paulista de Fitopatologia |
| record_format | dspace |
| spelling | INTA59122019-09-19T12:22:26Z Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina Gomez, Diana Erica Reis, Erlei Melo Soybeans Sporulation Plant Nutrition Soja Glycine Max Cercospora Sojina Esporulación Nutrición de las Plantas Fungi require special substrates for their isolation, vegetative growth and sporulation. In experiments conducted in the laboratory, the influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina conidia, the causal agent of soybean frogeye leaf spot, was assessed. The media potato sucrose agar, V-8 agar, tomato extract agar, soybean leaf extract agar, soybean seed extract agar, soybean meal agar, soybean flour agar and wheat flour agar were tested, added on the surface, with and without filter paper and under two light regimes, with 12 h light at 25°± 2°C and in the dark. A triple factorial 8x2x2 (substrates x light/dark x with/without filter paper) design with four replicates was used. V-8 agar medium was employed and the pH was adjusted with HCl 0.1N or NaOH 0.1N before autoclaving to the values: 3, 4, 5, 6, 7 and 8, and the pH of V-8 agar medium is 6.7. The evaluation was done on the seventh day of incubation. Data underwent regression analysis. Sporulation was maximized on the agar media V-8, seed extract, oat flour, tomato extract, and potato sucrose in the presence of filter paper and 12h light. On V-8 medium, maximal sporulation was obtained with pH 6.7. Os fungos requerem substratos especiais para seu isolamento, crescimento vegetativo e esporulação. Em experimentos conduzidos no laboratório foram avaliadas a influência de substratos, luz, papel de filtro e pH na esporulação de conídios de Cercospora sojina agente causal da mancha foliar olho-de-rã da soja. Foram testados os meios ágar batata sacarose, ágar V-8, ágar extrato de tomate, ágar extrato de folha de soja, ágar grãos de soja, ágar farelo de soja, ágar farinha soja e ágar farinha de aveia, acrescidos na superfície com e sem papel de filtro e submetidos a dois regimes luminosos, com luz de 12 h a 25°± 2°C e sem luz. O delineamento experimental usado foi fatorial triplo 8x2x2 (substratos x luz/escuro x com/sem papel de filtro), com quatro repetições distribuídas ao acaso. Usou-se o meio ágar V-8 ajustando o pH com HCl 0.1 N ou NaOH 0.1 N antes da autoclavagem nos valores: 3; 4; 5; 6; 7 e 8, sendo o pH do meio ágar V-8 de 6.7. A avaliação foi realizada aos sete dias de incubação. Os dados foram submetidos à análise da regressão. A esporulação foi maximizada nos meios V-8, ágar extrato de semente, farinha de aveia, extrato de tomate e batata sacarose ágar na presença de papel de filtro e fotoperíodo de 12 h. No meio V-8 a máxima esporulação foi obtida com o pH 6.7. EEA Sáenz Peña Fil: Gomez, Diana Erica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentina Fil: Reis, Erlei Melo. Universidad de Passo Fundo. Facultade de Agronomía y Medicina Veterinaria. Departamento de Fitosanidad; Brasil 2019-09-19T12:08:48Z 2019-09-19T12:08:48Z 2013 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5912 http://www.scielo.br/pdf/sp/v39n2/08.pdf 0100-5405 1980-5454 (Online) http://dx.doi.org/10.1590/S0100-54052013000200008 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Grupo Paulista de Fitopatologia Summa phytopathologica 39 (2) : 126-129. (2013) |
| spellingShingle | Soybeans Sporulation Plant Nutrition Soja Glycine Max Cercospora Sojina Esporulación Nutrición de las Plantas Gomez, Diana Erica Reis, Erlei Melo Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina |
| title | Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina |
| title_full | Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina |
| title_fullStr | Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina |
| title_full_unstemmed | Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina |
| title_short | Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina |
| title_sort | influence of substrates light filter paper and ph on the sporulation of cercospora sojina |
| topic | Soybeans Sporulation Plant Nutrition Soja Glycine Max Cercospora Sojina Esporulación Nutrición de las Plantas |
| url | http://hdl.handle.net/20.500.12123/5912 http://www.scielo.br/pdf/sp/v39n2/08.pdf http://dx.doi.org/10.1590/S0100-54052013000200008 |
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