Grapevine virus L: a novel vitivirus in grapevine
Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed r...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://link.springer.com/article/10.1007/s10658-019-01727-w http://hdl.handle.net/20.500.12123/5907 https://doi.org/10.1007/s10658-019-01727-w |
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| author | Debat, Humberto Julio Zavallo, Diego Brisbane, Reid Soltero Vončina, Darko Almeida, Rodrigo P. P. Blouin, Arnaud G. Al Rwahnih, Maher Gomez Talquenca, Gonzalo Asurmendi, Sebastian |
| author_browse | Al Rwahnih, Maher Almeida, Rodrigo P. P. Asurmendi, Sebastian Blouin, Arnaud G. Brisbane, Reid Soltero Debat, Humberto Julio Gomez Talquenca, Gonzalo Vončina, Darko Zavallo, Diego |
| author_facet | Debat, Humberto Julio Zavallo, Diego Brisbane, Reid Soltero Vončina, Darko Almeida, Rodrigo P. P. Blouin, Arnaud G. Al Rwahnih, Maher Gomez Talquenca, Gonzalo Asurmendi, Sebastian |
| author_sort | Debat, Humberto Julio |
| collection | INTA Digital |
| description | Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus. |
| format | Artículo |
| id | INTA5907 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | INTA59072019-09-19T11:06:47Z Grapevine virus L: a novel vitivirus in grapevine Debat, Humberto Julio Zavallo, Diego Brisbane, Reid Soltero Vončina, Darko Almeida, Rodrigo P. P. Blouin, Arnaud G. Al Rwahnih, Maher Gomez Talquenca, Gonzalo Asurmendi, Sebastian Vitivirus Betaflexiviridae Virus de las Plantas Vid Plant Viruses Grapevines Virus discovery Itivirus Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus. Instituto de Patología Vegetal Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Zaballo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Brisbane, Reid Soltero. Foundation Plant Services; Estados Unidos Fil: Vončina, Darko. University of Zagreb. Faculty of Agriculture. Department of Plant Pathology; Croacia Fil: Almeida, Rodrigo P. P. University of California. Department of Environmental Science, Policy and Management; Estados Unidos Fil: Blouin, Arnaud G.The New Zealand Institute for Plant & Food Research Limited; Nueva Zelanda Fil: Al Rwahnih, Maher. University of California. Department of Plant Pathology; Estados Unidos Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-09-19T10:48:43Z 2019-09-19T10:48:43Z 2019-04-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s10658-019-01727-w http://hdl.handle.net/20.500.12123/5907 0929-1873 1573-8469 (on line) https://doi.org/10.1007/s10658-019-01727-w eng info:eu-repo/semantics/restrictedAccess application/pdf European Journal of Plant Pathology 155 (1) : 319–328 (September 2019) |
| spellingShingle | Vitivirus Betaflexiviridae Virus de las Plantas Vid Plant Viruses Grapevines Virus discovery Itivirus Debat, Humberto Julio Zavallo, Diego Brisbane, Reid Soltero Vončina, Darko Almeida, Rodrigo P. P. Blouin, Arnaud G. Al Rwahnih, Maher Gomez Talquenca, Gonzalo Asurmendi, Sebastian Grapevine virus L: a novel vitivirus in grapevine |
| title | Grapevine virus L: a novel vitivirus in grapevine |
| title_full | Grapevine virus L: a novel vitivirus in grapevine |
| title_fullStr | Grapevine virus L: a novel vitivirus in grapevine |
| title_full_unstemmed | Grapevine virus L: a novel vitivirus in grapevine |
| title_short | Grapevine virus L: a novel vitivirus in grapevine |
| title_sort | grapevine virus l a novel vitivirus in grapevine |
| topic | Vitivirus Betaflexiviridae Virus de las Plantas Vid Plant Viruses Grapevines Virus discovery Itivirus |
| url | https://link.springer.com/article/10.1007/s10658-019-01727-w http://hdl.handle.net/20.500.12123/5907 https://doi.org/10.1007/s10658-019-01727-w |
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