Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)
Phytoplasmas are wall-less bacteria with a parasitic lifestyle responsible for numerous plant diseases worldwide. Since the introduction of next generation sequencing technologies, genome wide studies of these pathogens are flourishing and a handful of phytoplasma genomes are available in public dat...
| Autores principales: | , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/5891 https://link.springer.com/article/10.1007%2Fs40858-019-00293-0 https://doi.org/10.1007/s40858-019-00293-0 |
| _version_ | 1855035577405014016 |
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| author | Fernandez, Franco Daniel Debat, Humberto Julio Conci, Luis Rogelio |
| author_browse | Conci, Luis Rogelio Debat, Humberto Julio Fernandez, Franco Daniel |
| author_facet | Fernandez, Franco Daniel Debat, Humberto Julio Conci, Luis Rogelio |
| author_sort | Fernandez, Franco Daniel |
| collection | INTA Digital |
| description | Phytoplasmas are wall-less bacteria with a parasitic lifestyle responsible for numerous plant diseases worldwide. Since the introduction of next generation sequencing technologies, genome wide studies of these pathogens are flourishing and a handful of phytoplasma genomes are available in public databases. In South America, phytoplasmas from the 16SrIII group (X-disease) are the most widely distributed, but only one draft genome from a 16SrIII-J phytoplasma-infected periwinkle plant has been generated (phytoplasma Vc33). Here, in grafting experiments, we characterized the phenotypic signatures of an Argentinian daisy-derived isolate of a 16SrIII-J phytoplasma (Bellis virescence phytoplasma) infecting periwinkle. Moreover, we applied a pipeline for genome-wide annotation of the Vc33 genome and identified the effector protein SAP54. We then employed the obtained data to amplify, clone, sequence and characterize a SAP54 orthologue protein from the Bellis virescence phytoplasma. Structural analyses suggest that the identified SAP54 effector is highly conserved. The results gathered here could provide the basis for reverse genetics experiments using 16SrIII-J SAP54 proteins to assess their eventual role in pathogenesis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5891 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA58912022-04-20T16:05:13Z Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J) Fernandez, Franco Daniel Debat, Humberto Julio Conci, Luis Rogelio Argentina Effectors Fitoplasmas Efectores Moleculares Genética Molecular Phytoplasmas Molecular Genetics X-disease SAP54 16SrIII-J Phytoplasmas are wall-less bacteria with a parasitic lifestyle responsible for numerous plant diseases worldwide. Since the introduction of next generation sequencing technologies, genome wide studies of these pathogens are flourishing and a handful of phytoplasma genomes are available in public databases. In South America, phytoplasmas from the 16SrIII group (X-disease) are the most widely distributed, but only one draft genome from a 16SrIII-J phytoplasma-infected periwinkle plant has been generated (phytoplasma Vc33). Here, in grafting experiments, we characterized the phenotypic signatures of an Argentinian daisy-derived isolate of a 16SrIII-J phytoplasma (Bellis virescence phytoplasma) infecting periwinkle. Moreover, we applied a pipeline for genome-wide annotation of the Vc33 genome and identified the effector protein SAP54. We then employed the obtained data to amplify, clone, sequence and characterize a SAP54 orthologue protein from the Bellis virescence phytoplasma. Structural analyses suggest that the identified SAP54 effector is highly conserved. The results gathered here could provide the basis for reverse genetics experiments using 16SrIII-J SAP54 proteins to assess their eventual role in pathogenesis. Instituto de Patología Vegetal Fil: Fernández, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2019-09-18T10:46:00Z 2019-09-18T10:46:00Z 2019-06-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5891 https://link.springer.com/article/10.1007%2Fs40858-019-00293-0 1983-2052 https://doi.org/10.1007/s40858-019-00293-0 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Tropical Plant Pathology 44 (4) : 392-397 (August 2019) |
| spellingShingle | Argentina Effectors Fitoplasmas Efectores Moleculares Genética Molecular Phytoplasmas Molecular Genetics X-disease SAP54 16SrIII-J Fernandez, Franco Daniel Debat, Humberto Julio Conci, Luis Rogelio Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J) |
| title | Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J) |
| title_full | Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J) |
| title_fullStr | Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J) |
| title_full_unstemmed | Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J) |
| title_short | Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J) |
| title_sort | molecular characterization of effector protein sap54 in bellis virescence phytoplasma 16sriii j |
| topic | Argentina Effectors Fitoplasmas Efectores Moleculares Genética Molecular Phytoplasmas Molecular Genetics X-disease SAP54 16SrIII-J |
| url | http://hdl.handle.net/20.500.12123/5891 https://link.springer.com/article/10.1007%2Fs40858-019-00293-0 https://doi.org/10.1007/s40858-019-00293-0 |
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