Effect of long-term mineral fertilisation on soil microbial abundance, community structure and diversity in a Typic Hapludoll under intensive farming systems
Fertiliser application can not only influence plant communities, but also the soil microbial community dynamics, and consequently soil quality. Specifically, mineral fertilisation can directly or indirectly affect soil chemical properties, microbial abundance and, the structure and diversity of s...
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| Format: | Artículo |
| Language: | Inglés |
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Wiley
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/5889 https://onlinelibrary.wiley.com/doi/full/10.1111/aab.12546 https://doi.org/10.1111/aab.12546 |
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| author | Verdenelli, Romina Aylen Dominchin, Maria Florencia Perez Brandan, Carolina Rovea, Adrián Vargas Gil, Silvina Meriles, Jose Manuel |
| author_browse | Dominchin, Maria Florencia Meriles, Jose Manuel Perez Brandan, Carolina Rovea, Adrián Vargas Gil, Silvina Verdenelli, Romina Aylen |
| author_facet | Verdenelli, Romina Aylen Dominchin, Maria Florencia Perez Brandan, Carolina Rovea, Adrián Vargas Gil, Silvina Meriles, Jose Manuel |
| author_sort | Verdenelli, Romina Aylen |
| collection | INTA Digital |
| description | Fertiliser application can not only influence plant communities, but also the soil microbial
community dynamics, and consequently soil quality. Specifically, mineral fertilisation can
directly or indirectly affect soil chemical properties, microbial abundance and, the structure
and diversity of soil microbial communities. We investigated the impact of six different
mineral fertiliser regimes in a maize/soybean rotation system: control (CK,
without fertilisation), PS (application of phosphorus plus sulphur), NS (application of
nitrogen plus S), NP (application of N plus P), NPS (application of N, P plus S) and NPSm
(application of N, P, S plus micronutrients). Soil samples were collected at the physiological
maturity stage of maize and soybean in March of 2013 and 2014, respectively. Overall,
mineral fertilisation resulted in significantly decreased soil pH and increased total
organic carbon compared with the control (CK). The analysis of terminal restriction fragment
length polymorphism (T-RFLP) revealed that mineral fertilisers caused a shift in
the composition of both bacterial and fungal communities. In 2013, the highest value of
Shannon diversity of bacterial terminal restriction fragments (TRFs) was found in control
soils. In 2014, NPSm treated soils showed the lowest values of diversity for both bacterial
and fungal TRFs. In both crop growing seasons, the analysis of phospholipid fatty
acid (PLFA) detected the lowest value of total microbial biomass under CK. As PLFA
analysis can be used to evaluate total microbial community, this result suggests that
fertilisation increased total microbial biomass. When the bacterial and fungal abundance
were examined using real time polymerase chain reaction, the results revealed that mineral
fertilisation led to decreased bacterial abundance (16S rRNA), while fungal abundance
(18S rRNA) was found to be increased in both crop growing seasons. Our results
show that mineral fertiliser application has a significant impact on soil properties, bacterial
and fungal abundance and microbial diversity. However, further studies are needed
to better understand the mechanisms involved in the changes to microbial communities
as a consequence of mineral fertilisation. |
| format | Artículo |
| id | INTA5889 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA58892019-09-17T15:03:36Z Effect of long-term mineral fertilisation on soil microbial abundance, community structure and diversity in a Typic Hapludoll under intensive farming systems Verdenelli, Romina Aylen Dominchin, Maria Florencia Perez Brandan, Carolina Rovea, Adrián Vargas Gil, Silvina Meriles, Jose Manuel Intensive Farming Soil Microorganisms Soil Chemicophysical Properties Explotación Agrícola Intensiva Microorganismos del Suelo Propiedades Físico-Químicas Suelo Mineral Fertilisation Fertilización Mineral Hapludoles Fertiliser application can not only influence plant communities, but also the soil microbial community dynamics, and consequently soil quality. Specifically, mineral fertilisation can directly or indirectly affect soil chemical properties, microbial abundance and, the structure and diversity of soil microbial communities. We investigated the impact of six different mineral fertiliser regimes in a maize/soybean rotation system: control (CK, without fertilisation), PS (application of phosphorus plus sulphur), NS (application of nitrogen plus S), NP (application of N plus P), NPS (application of N, P plus S) and NPSm (application of N, P, S plus micronutrients). Soil samples were collected at the physiological maturity stage of maize and soybean in March of 2013 and 2014, respectively. Overall, mineral fertilisation resulted in significantly decreased soil pH and increased total organic carbon compared with the control (CK). The analysis of terminal restriction fragment length polymorphism (T-RFLP) revealed that mineral fertilisers caused a shift in the composition of both bacterial and fungal communities. In 2013, the highest value of Shannon diversity of bacterial terminal restriction fragments (TRFs) was found in control soils. In 2014, NPSm treated soils showed the lowest values of diversity for both bacterial and fungal TRFs. In both crop growing seasons, the analysis of phospholipid fatty acid (PLFA) detected the lowest value of total microbial biomass under CK. As PLFA analysis can be used to evaluate total microbial community, this result suggests that fertilisation increased total microbial biomass. When the bacterial and fungal abundance were examined using real time polymerase chain reaction, the results revealed that mineral fertilisation led to decreased bacterial abundance (16S rRNA), while fungal abundance (18S rRNA) was found to be increased in both crop growing seasons. Our results show that mineral fertiliser application has a significant impact on soil properties, bacterial and fungal abundance and microbial diversity. However, further studies are needed to better understand the mechanisms involved in the changes to microbial communities as a consequence of mineral fertilisation. EEA Salta Fil: Verdenelli, Romina Aylen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Ciencias y Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Dominchin, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Ciencias y Tecnología de los Alimentos; Argentina Fil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Rovea, Adrián. Consorcio Regional de Experimentación Agrícola (CREA). Región CREA Santa Fe; Argentina Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Patología Vegetal; Argentina Fil: Meriles, Jose Manuel. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Ciencias y Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina 2019-09-17T14:54:31Z 2019-09-17T14:54:31Z 2019-09-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5889 https://onlinelibrary.wiley.com/doi/full/10.1111/aab.12546 1744-7348 https://doi.org/10.1111/aab.12546 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Annals of applied biology (03 September 2019) |
| spellingShingle | Intensive Farming Soil Microorganisms Soil Chemicophysical Properties Explotación Agrícola Intensiva Microorganismos del Suelo Propiedades Físico-Químicas Suelo Mineral Fertilisation Fertilización Mineral Hapludoles Verdenelli, Romina Aylen Dominchin, Maria Florencia Perez Brandan, Carolina Rovea, Adrián Vargas Gil, Silvina Meriles, Jose Manuel Effect of long-term mineral fertilisation on soil microbial abundance, community structure and diversity in a Typic Hapludoll under intensive farming systems |
| title | Effect of long-term mineral fertilisation on soil microbial abundance, community structure and diversity in a Typic Hapludoll under intensive farming systems |
| title_full | Effect of long-term mineral fertilisation on soil microbial abundance, community structure and diversity in a Typic Hapludoll under intensive farming systems |
| title_fullStr | Effect of long-term mineral fertilisation on soil microbial abundance, community structure and diversity in a Typic Hapludoll under intensive farming systems |
| title_full_unstemmed | Effect of long-term mineral fertilisation on soil microbial abundance, community structure and diversity in a Typic Hapludoll under intensive farming systems |
| title_short | Effect of long-term mineral fertilisation on soil microbial abundance, community structure and diversity in a Typic Hapludoll under intensive farming systems |
| title_sort | effect of long term mineral fertilisation on soil microbial abundance community structure and diversity in a typic hapludoll under intensive farming systems |
| topic | Intensive Farming Soil Microorganisms Soil Chemicophysical Properties Explotación Agrícola Intensiva Microorganismos del Suelo Propiedades Físico-Químicas Suelo Mineral Fertilisation Fertilización Mineral Hapludoles |
| url | http://hdl.handle.net/20.500.12123/5889 https://onlinelibrary.wiley.com/doi/full/10.1111/aab.12546 https://doi.org/10.1111/aab.12546 |
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