Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu
Rachiplusia nu (Lepidoptera: Noctuidae) is a key soybean pest in Argentina. Current management of this moth relies mainly on the use of synthetic insecticides and transgenic plants. In search of biological control-based alternatives, a baculovirus from R. nu (hereafter RanuNPV) was characterized a...
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/5825 https://www.sciencedirect.com/science/article/pii/S0022201119300850?via%3Dihub https://doi.org/10.1016/j.jip.2019.107211 |
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| author | Jakubowicz, Violeta Taibo, Catalina Beatriz Sciocco, Alicia Ines Arneodo Larochette, Joel Demian |
| author_browse | Arneodo Larochette, Joel Demian Jakubowicz, Violeta Sciocco, Alicia Ines Taibo, Catalina Beatriz |
| author_facet | Jakubowicz, Violeta Taibo, Catalina Beatriz Sciocco, Alicia Ines Arneodo Larochette, Joel Demian |
| author_sort | Jakubowicz, Violeta |
| collection | INTA Digital |
| description | Rachiplusia nu (Lepidoptera: Noctuidae) is a key soybean pest in Argentina. Current management of this moth
relies mainly on the use of synthetic insecticides and transgenic plants. In search of biological control-based
alternatives, a baculovirus from R. nu (hereafter RanuNPV) was characterized and its insecticidal properties
tested under laboratory conditions. RanuNPV occlusion bodies (OBs) were nearly tetrahedral, averaging
1.0 ± 0.2 μm in their longest edge and containing singly enveloped nucleocapsids. Histopathology of infected
late-instar larvae revealed broad tissue tropism, where fat bodies and epidermis were the most affected organs.
Phylogenetic analysis of concatenated polh, lef-8 and lef-9 partial sequences classified RanuNPV as a new species
that clusters with other group II alphabaculoviruses infecting larvae of Plusiinae. Bioassays performed with R. nu
neonates determined the median lethal dosage to be approximately 2.5 OBs/larva; most insects died within
4–5 days post inoculation showing typical baculovirus-induced liquefaction. No effects were observed in other
lepidopteran species assayed, including Spodoptera frugiperda, Cydia pomonella and Diatraea saccharalis. High
pathogenicity and host specificity make RanuNPV a good candidate for controlling R. nu. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5825 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA58252019-09-09T17:16:55Z Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu Jakubowicz, Violeta Taibo, Catalina Beatriz Sciocco, Alicia Ines Arneodo Larochette, Joel Demian Rachiplusia Baculoviridae Organismos Beneficiosos Patogénesis Filogenia Soja Glycine Max Agentes de Control Biológico Lepidoptera Beneficial Organisms Pathogenesis Phylogeny Soybeans Biological Control Agents Viral Insecticide Rachiplusia nu (Lepidoptera: Noctuidae) is a key soybean pest in Argentina. Current management of this moth relies mainly on the use of synthetic insecticides and transgenic plants. In search of biological control-based alternatives, a baculovirus from R. nu (hereafter RanuNPV) was characterized and its insecticidal properties tested under laboratory conditions. RanuNPV occlusion bodies (OBs) were nearly tetrahedral, averaging 1.0 ± 0.2 μm in their longest edge and containing singly enveloped nucleocapsids. Histopathology of infected late-instar larvae revealed broad tissue tropism, where fat bodies and epidermis were the most affected organs. Phylogenetic analysis of concatenated polh, lef-8 and lef-9 partial sequences classified RanuNPV as a new species that clusters with other group II alphabaculoviruses infecting larvae of Plusiinae. Bioassays performed with R. nu neonates determined the median lethal dosage to be approximately 2.5 OBs/larva; most insects died within 4–5 days post inoculation showing typical baculovirus-induced liquefaction. No effects were observed in other lepidopteran species assayed, including Spodoptera frugiperda, Cydia pomonella and Diatraea saccharalis. High pathogenicity and host specificity make RanuNPV a good candidate for controlling R. nu. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Jakubowicz, Violeta. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de investigaciones Científicas y Tecnológicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Servicio de Microscopía; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de investigaciones Científicas y Tecnológicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina 2019-09-09T17:12:04Z 2019-09-09T17:12:04Z 2019-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5825 https://www.sciencedirect.com/science/article/pii/S0022201119300850?via%3Dihub 0022-2011 https://doi.org/10.1016/j.jip.2019.107211 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of invertebrate pathology 166 : 107211. (September 2019) |
| spellingShingle | Rachiplusia Baculoviridae Organismos Beneficiosos Patogénesis Filogenia Soja Glycine Max Agentes de Control Biológico Lepidoptera Beneficial Organisms Pathogenesis Phylogeny Soybeans Biological Control Agents Viral Insecticide Jakubowicz, Violeta Taibo, Catalina Beatriz Sciocco, Alicia Ines Arneodo Larochette, Joel Demian Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu |
| title | Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu |
| title_full | Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu |
| title_fullStr | Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu |
| title_full_unstemmed | Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu |
| title_short | Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu |
| title_sort | biological and molecular characterization of rachiplusia nu single nucleopolyhedrovirus a promising biocontrol agent against the south american soybean pest rachiplusia nu |
| topic | Rachiplusia Baculoviridae Organismos Beneficiosos Patogénesis Filogenia Soja Glycine Max Agentes de Control Biológico Lepidoptera Beneficial Organisms Pathogenesis Phylogeny Soybeans Biological Control Agents Viral Insecticide |
| url | http://hdl.handle.net/20.500.12123/5825 https://www.sciencedirect.com/science/article/pii/S0022201119300850?via%3Dihub https://doi.org/10.1016/j.jip.2019.107211 |
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