Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska
A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press; Weed Science Society of America
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/5698 https://www.cambridge.org/core/journals/weed-science/article/multipleherbicide-resistance-in-a-24dresistant-waterhemp-amaranthus-tuberculatus-population-from-nebraska/9309AB3614CF11C833C658CE65CA1114 https://doi.org/10.1017/wsc.2017.39 |
| _version_ | 1855035544654839808 |
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| author | Crespo, Roberto Javier Wingeyer, Ana Beatriz Kruger, Greg R. Riggins, Chance W. Tranel, Patrick J. Bernards, Mark L. |
| author_browse | Bernards, Mark L. Crespo, Roberto Javier Kruger, Greg R. Riggins, Chance W. Tranel, Patrick J. Wingeyer, Ana Beatriz |
| author_facet | Crespo, Roberto Javier Wingeyer, Ana Beatriz Kruger, Greg R. Riggins, Chance W. Tranel, Patrick J. Bernards, Mark L. |
| author_sort | Crespo, Roberto Javier |
| collection | INTA Digital |
| description | A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose–response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase–inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non–target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5698 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Cambridge University Press; Weed Science Society of America |
| publisherStr | Cambridge University Press; Weed Science Society of America |
| record_format | dspace |
| spelling | INTA56982025-12-17T14:06:32Z Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska Crespo, Roberto Javier Wingeyer, Ana Beatriz Kruger, Greg R. Riggins, Chance W. Tranel, Patrick J. Bernards, Mark L. Resistencia a los Herbicidas Resistencia Cruzada Atrazina Mesotriona Glufosinato Glifosato Resistance to Herbicides Cross Resistance Atrazine Mesotrione Glufosinate Glyphosate Amaranthus Tuberculatus Nebraska, Estados Unidos Cáñamo de Agua 2,4-D Waterhemp Imazethapyr Lactofen Aminocyclopyrachlor A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose–response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase–inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non–target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors. EEA Paraná Fil: Crespo, Roberto J. University of Nebraska–Lincoln. Department of Agronomy and Horticulture; Estados Unidos Fil: Wingeyer, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kruger, Greg R. University of Nebraska–Lincoln. West Central Research and Extension Center; Estados Unidos Fil: Riggins, Chance W. University of Illinois. Department of Crop Sciences; Estados Unidos Fil: Tranel, Patrick J. University of Illinois. Department of Crop Sciences; Estados Unidos Fil: Bernards, Mark L. University of Nebraska–Lincoln. Department of Agronomy and Horticulture; Estados Unidos 2019-08-27T12:35:08Z 2019-08-27T12:35:08Z 2017-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5698 https://www.cambridge.org/core/journals/weed-science/article/multipleherbicide-resistance-in-a-24dresistant-waterhemp-amaranthus-tuberculatus-population-from-nebraska/9309AB3614CF11C833C658CE65CA1114 0043-1745 1550-2759 (Online) https://doi.org/10.1017/wsc.2017.39 eng info:eu-repo/semantics/restrictedAccess application/pdf Cambridge University Press; Weed Science Society of America Weed Science 65 (6) :743–754. (November 2017) |
| spellingShingle | Resistencia a los Herbicidas Resistencia Cruzada Atrazina Mesotriona Glufosinato Glifosato Resistance to Herbicides Cross Resistance Atrazine Mesotrione Glufosinate Glyphosate Amaranthus Tuberculatus Nebraska, Estados Unidos Cáñamo de Agua 2,4-D Waterhemp Imazethapyr Lactofen Aminocyclopyrachlor Crespo, Roberto Javier Wingeyer, Ana Beatriz Kruger, Greg R. Riggins, Chance W. Tranel, Patrick J. Bernards, Mark L. Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska |
| title | Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska |
| title_full | Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska |
| title_fullStr | Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska |
| title_full_unstemmed | Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska |
| title_short | Multiple-Herbicide resistance in a 2,4-D–Resistant waterhemp (Amaranthus tuberculatus) population from Nebraska |
| title_sort | multiple herbicide resistance in a 2 4 d resistant waterhemp amaranthus tuberculatus population from nebraska |
| topic | Resistencia a los Herbicidas Resistencia Cruzada Atrazina Mesotriona Glufosinato Glifosato Resistance to Herbicides Cross Resistance Atrazine Mesotrione Glufosinate Glyphosate Amaranthus Tuberculatus Nebraska, Estados Unidos Cáñamo de Agua 2,4-D Waterhemp Imazethapyr Lactofen Aminocyclopyrachlor |
| url | http://hdl.handle.net/20.500.12123/5698 https://www.cambridge.org/core/journals/weed-science/article/multipleherbicide-resistance-in-a-24dresistant-waterhemp-amaranthus-tuberculatus-population-from-nebraska/9309AB3614CF11C833C658CE65CA1114 https://doi.org/10.1017/wsc.2017.39 |
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