Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida
Progression of leaf senescence depends on several families of transcription factors. In Arabidopsis, the NAC family plays crucial roles in the modulation of leaf senescence; however, the mechanisms involved in this NAC-mediated regulation have not been extensively explored in agronomic species. Petu...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0168945219305862 http://hdl.handle.net/20.500.12123/5683 https://doi.org/10.1016/j.plantsci.2019.110195 |
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| author | Trupkin, Santiago Ariel Astigueta, Francisco H. Baigorria, Amilcar Hernan Garcia, Martin Nahuel Delfosse, Veronica Cecilia González, Sergio A. Perez De La Torre, Mariana Moschen, Sebastian Lia, Veronica Viviana Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_browse | Astigueta, Francisco H. Baigorria, Amilcar Hernan Delfosse, Veronica Cecilia Fernandez, Paula Del Carmen Garcia, Martin Nahuel González, Sergio A. Heinz, Ruth Amelia Lia, Veronica Viviana Moschen, Sebastian Perez De La Torre, Mariana Trupkin, Santiago Ariel |
| author_facet | Trupkin, Santiago Ariel Astigueta, Francisco H. Baigorria, Amilcar Hernan Garcia, Martin Nahuel Delfosse, Veronica Cecilia González, Sergio A. Perez De La Torre, Mariana Moschen, Sebastian Lia, Veronica Viviana Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_sort | Trupkin, Santiago Ariel |
| collection | INTA Digital |
| description | Progression of leaf senescence depends on several families of transcription factors. In Arabidopsis, the NAC family plays crucial roles in the modulation of leaf senescence; however, the mechanisms involved in this NAC-mediated regulation have not been extensively explored in agronomic species. Petunia hybrida is an ornamental plant that is commonly found worldwide. Decreasing the rate of leaf and petal senescence in P. hybrida is essential for maintaining plant quality. In this study, we examined the NAC-mediated networks involved in regulating senescence in this species. From 41 NAC genes, the expression of which changed in Arabidopsis during leaf senescence, we identified 29 putative orthologs in P. hybrida. Analysis using quantitative real-time-PCR indicated that 24 genes in P. hybrida changed their transcript levels during natural leaf senescence. Leaf-expressed genes were subsequently assessed in petals undergoing natural and pollination-induced senescence. Expression data and phylogenetic analysis were used to generate a list of 10–15 candidate genes; 7 of these were considered key regulatory candidates in senescence because of their consistent upregulation in the three senescence processes examined. Altogether, we identified common and distinct patterns of gene expression at different stages of leaf and petal development and during progression of senescence. The results obtained in this study will contribute to the understanding of NAC-mediated regulatory networks in petunia. |
| format | Artículo |
| id | INTA5683 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA56832022-01-14T12:52:09Z Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida Trupkin, Santiago Ariel Astigueta, Francisco H. Baigorria, Amilcar Hernan Garcia, Martin Nahuel Delfosse, Veronica Cecilia González, Sergio A. Perez De La Torre, Mariana Moschen, Sebastian Lia, Veronica Viviana Fernandez, Paula Del Carmen Heinz, Ruth Amelia Petunia hybrida Fitomejoramiento Genética Factores de Transcripción Expresión Génica Avejentamiento Plant Breeding Genetics Transcription Factors Gene Expression Senescence Progression of leaf senescence depends on several families of transcription factors. In Arabidopsis, the NAC family plays crucial roles in the modulation of leaf senescence; however, the mechanisms involved in this NAC-mediated regulation have not been extensively explored in agronomic species. Petunia hybrida is an ornamental plant that is commonly found worldwide. Decreasing the rate of leaf and petal senescence in P. hybrida is essential for maintaining plant quality. In this study, we examined the NAC-mediated networks involved in regulating senescence in this species. From 41 NAC genes, the expression of which changed in Arabidopsis during leaf senescence, we identified 29 putative orthologs in P. hybrida. Analysis using quantitative real-time-PCR indicated that 24 genes in P. hybrida changed their transcript levels during natural leaf senescence. Leaf-expressed genes were subsequently assessed in petals undergoing natural and pollination-induced senescence. Expression data and phylogenetic analysis were used to generate a list of 10–15 candidate genes; 7 of these were considered key regulatory candidates in senescence because of their consistent upregulation in the three senescence processes examined. Altogether, we identified common and distinct patterns of gene expression at different stages of leaf and petal development and during progression of senescence. The results obtained in this study will contribute to the understanding of NAC-mediated regulatory networks in petunia. Instituto de Floricultura Fil: Trupkin, Santiago Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Astigueta, Francisco H. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Baigorria, Amilcar Hernan. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Delfosse, Verónica C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: González, Sergio A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina Fil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2019-08-26T11:30:13Z 2019-08-26T11:30:13Z 2019-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0168945219305862 http://hdl.handle.net/20.500.12123/5683 0168-9452 https://doi.org/10.1016/j.plantsci.2019.110195 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Plant Science 287 : 110195 (October 2019) |
| spellingShingle | Petunia hybrida Fitomejoramiento Genética Factores de Transcripción Expresión Génica Avejentamiento Plant Breeding Genetics Transcription Factors Gene Expression Senescence Trupkin, Santiago Ariel Astigueta, Francisco H. Baigorria, Amilcar Hernan Garcia, Martin Nahuel Delfosse, Veronica Cecilia González, Sergio A. Perez De La Torre, Mariana Moschen, Sebastian Lia, Veronica Viviana Fernandez, Paula Del Carmen Heinz, Ruth Amelia Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida |
| title | Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida |
| title_full | Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida |
| title_fullStr | Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida |
| title_full_unstemmed | Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida |
| title_short | Identification and expression analysis of NAC transcription factors potentially involved in leaf and petal senescence in Petunia hybrida |
| title_sort | identification and expression analysis of nac transcription factors potentially involved in leaf and petal senescence in petunia hybrida |
| topic | Petunia hybrida Fitomejoramiento Genética Factores de Transcripción Expresión Génica Avejentamiento Plant Breeding Genetics Transcription Factors Gene Expression Senescence |
| url | https://www.sciencedirect.com/science/article/pii/S0168945219305862 http://hdl.handle.net/20.500.12123/5683 https://doi.org/10.1016/j.plantsci.2019.110195 |
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