In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol
This study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main ter...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2019
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/5679 https://link.springer.com/article/10.1007%2Fs11240-019-01641-3 https://doi.org/10.1007/s11240-019-01641-3 |
| _version_ | 1855483668186791936 |
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| author | Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí |
| author_browse | Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí |
| author_facet | Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí |
| author_sort | Escoriaza, Maria Georgina |
| collection | INTA Digital |
| description | This study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main terpene identified by gas chromatography electron impact mass spectrometry (GC-EIMS) was nerolidol, which was found in apical and basal tissues at a concentration of ca. 0.12 μg mg FW−1. Consistent with this, an increment in terpene synthase (TPS) activity, assessed as tritiated farnesyl pyrophosphate ([1−3H]-FPP) transformed into hexane-soluble radioactive products in infected plants, was observed. TPS activity increased in correlation with the fungal concentration. Nerolidol inhibited in vitro mycelium growth and reduced fungal growth, in a concentration dependent manner. According to our results, the response of grapevine tissues to Pm. parasiticum is systemic, as it can be visualized by an augment of transcript abundance of VvPNLinNer1. It is also characterized by an increase of de novo synthesis of TPS responsible for the biosynthesis of phytoalexin nerolidol. |
| format | Artículo |
| id | INTA5679 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA56792019-08-23T13:14:37Z In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí Vitis Vinífera Vitroplantas Fitoalexina Respuesta Inmunológica Infección Enfermedades Fungosas Terpenoidos Vitroplants Phytoalexins Immune Response Infection Fungal Diseases Terpenoids Phaeoacremonium Parasiticum Nerolidol Terpene Synthase Activity This study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main terpene identified by gas chromatography electron impact mass spectrometry (GC-EIMS) was nerolidol, which was found in apical and basal tissues at a concentration of ca. 0.12 μg mg FW−1. Consistent with this, an increment in terpene synthase (TPS) activity, assessed as tritiated farnesyl pyrophosphate ([1−3H]-FPP) transformed into hexane-soluble radioactive products in infected plants, was observed. TPS activity increased in correlation with the fungal concentration. Nerolidol inhibited in vitro mycelium growth and reduced fungal growth, in a concentration dependent manner. According to our results, the response of grapevine tissues to Pm. parasiticum is systemic, as it can be visualized by an augment of transcript abundance of VvPNLinNer1. It is also characterized by an increase of de novo synthesis of TPS responsible for the biosynthesis of phytoalexin nerolidol. EEA Mendoza Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina 2019-08-23T13:06:25Z 2019-08-23T13:06:25Z 2019-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5679 https://link.springer.com/article/10.1007%2Fs11240-019-01641-3 Escoriaza, G., García Lampasona, S., Gomez Talquenca, S. et al. Plant Cell Tiss Organ Cult (2019) 138: 459. https://doi.org/10.1007/s11240-019-01641-3 0167-6857 1573-5044 (Online) https://doi.org/10.1007/s11240-019-01641-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant cell, tissue and organ culture: PCTOC 138 (3) : 459–466. (September 2019) |
| spellingShingle | Vitis Vinífera Vitroplantas Fitoalexina Respuesta Inmunológica Infección Enfermedades Fungosas Terpenoidos Vitroplants Phytoalexins Immune Response Infection Fungal Diseases Terpenoids Phaeoacremonium Parasiticum Nerolidol Terpene Synthase Activity Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title | In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_full | In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_fullStr | In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_full_unstemmed | In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_short | In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_sort | in vitro plants of vitis vinifera respond to infection with the fungus phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| topic | Vitis Vinífera Vitroplantas Fitoalexina Respuesta Inmunológica Infección Enfermedades Fungosas Terpenoidos Vitroplants Phytoalexins Immune Response Infection Fungal Diseases Terpenoids Phaeoacremonium Parasiticum Nerolidol Terpene Synthase Activity |
| url | http://hdl.handle.net/20.500.12123/5679 https://link.springer.com/article/10.1007%2Fs11240-019-01641-3 https://doi.org/10.1007/s11240-019-01641-3 |
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