Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
The work describes a case of Sarcocystis gigantea infection in a 3-years-old Corriedale ewe from Buenos Aires Province, Argentina. The ewe was found dead with a poor body condition. Pathological and molecular studies were carried out in order to try and confirm the causative agent of the infection....
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0304401717304648?via%3Dihub#! http://hdl.handle.net/20.500.12123/5601 https://doi.org/10.1016/j.vetpar.2017.10.017 |
| _version_ | 1855035526796541952 |
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| author | Gual, Ignacio Bartley, Paul Katzer, Frank Innes, Elisabeth Canton, German Jose Moore, Prando Dadin |
| author_browse | Bartley, Paul Canton, German Jose Gual, Ignacio Innes, Elisabeth Katzer, Frank Moore, Prando Dadin |
| author_facet | Gual, Ignacio Bartley, Paul Katzer, Frank Innes, Elisabeth Canton, German Jose Moore, Prando Dadin |
| author_sort | Gual, Ignacio |
| collection | INTA Digital |
| description | The work describes a case of Sarcocystis gigantea infection in a 3-years-old Corriedale ewe from Buenos Aires Province, Argentina. The ewe was found dead with a poor body condition. Pathological and molecular studies were carried out in order to try and confirm the causative agent of the infection. At necropsy, approx. 100 whitish elliptic (3–5 mm to 5–8 mm) macrocysts with a hard consistency were observed along the esophageal and pharyngeal muscular layers. Microscopically, the macrocysts consisted of an eosinophilic wall, internal septa originated from the eosinophilic wall and basophilic parasitic cells were located among the septa. The sarcocysts were identified molecularly through PCR amplification and sequencing of a short segment of the 18S rRNA gene. Sequence analysis of the amplified DNA demonstrated 100% identity to S. gigantea sequences previously published. To our knowledge this is the first molecular confirmation of S. gigantea infection in sheep in the Americas. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5601 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA56012019-08-08T14:33:05Z Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report. Gual, Ignacio Bartley, Paul Katzer, Frank Innes, Elisabeth Canton, German Jose Moore, Prando Dadin Sarcocystis Oveja PCR Argentina Ewes The work describes a case of Sarcocystis gigantea infection in a 3-years-old Corriedale ewe from Buenos Aires Province, Argentina. The ewe was found dead with a poor body condition. Pathological and molecular studies were carried out in order to try and confirm the causative agent of the infection. At necropsy, approx. 100 whitish elliptic (3–5 mm to 5–8 mm) macrocysts with a hard consistency were observed along the esophageal and pharyngeal muscular layers. Microscopically, the macrocysts consisted of an eosinophilic wall, internal septa originated from the eosinophilic wall and basophilic parasitic cells were located among the septa. The sarcocysts were identified molecularly through PCR amplification and sequencing of a short segment of the 18S rRNA gene. Sequence analysis of the amplified DNA demonstrated 100% identity to S. gigantea sequences previously published. To our knowledge this is the first molecular confirmation of S. gigantea infection in sheep in the Americas. EEA Balcarce Fil: Gual, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bartley, Paul. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido Fil: Katzer, Frank. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido Fil: Innes, Elisabeth. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido Fil: Cantón, Germán. INTA, Estación Experimental Agropecuaria Balcarce; Argentina Fil: Moore, Dadín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-08-08T14:23:42Z 2019-08-08T14:23:42Z 2017-12-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0304401717304648?via%3Dihub#! http://hdl.handle.net/20.500.12123/5601 0304-4017 https://doi.org/10.1016/j.vetpar.2017.10.017 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary parasitology 248 : 25-27. (15 December 2017) |
| spellingShingle | Sarcocystis Oveja PCR Argentina Ewes Gual, Ignacio Bartley, Paul Katzer, Frank Innes, Elisabeth Canton, German Jose Moore, Prando Dadin Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report. |
| title | Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report. |
| title_full | Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report. |
| title_fullStr | Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report. |
| title_full_unstemmed | Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report. |
| title_short | Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report. |
| title_sort | molecular confirmation of sarcocystis gigantean in a naturally infected sheep in argentina a case report |
| topic | Sarcocystis Oveja PCR Argentina Ewes |
| url | https://www.sciencedirect.com/science/article/pii/S0304401717304648?via%3Dihub#! http://hdl.handle.net/20.500.12123/5601 https://doi.org/10.1016/j.vetpar.2017.10.017 |
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