Mycobacterium bovis requires P27 (LprG) to arrest phagosome maturation and replicate within bovine macrophages
Mycobacterium bovis causes tuberculosis in a wide variety of mammals, with strong tropism for cattle and eventually humans. P27, also called LprG, is among the proteins involved in the mechanisms of the virulence and persistence of M. bovis and Mycobacterium tuberculosis. Here, we describe a novel...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/552 http://iai.asm.org/content/85/3/e00720-16.full.pdf+html https://doi.org/10.1128/IAI.00720-16 |
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| author | Vazquez, Cristina Lourdes Bianco, María Veronica Blanco, Federico Carlos Forrellad, Marina Andrea Gutierrez, Maximiliano Gabriel Bigi, Fabiana |
| author_browse | Bianco, María Veronica Bigi, Fabiana Blanco, Federico Carlos Forrellad, Marina Andrea Gutierrez, Maximiliano Gabriel Vazquez, Cristina Lourdes |
| author_facet | Vazquez, Cristina Lourdes Bianco, María Veronica Blanco, Federico Carlos Forrellad, Marina Andrea Gutierrez, Maximiliano Gabriel Bigi, Fabiana |
| author_sort | Vazquez, Cristina Lourdes |
| collection | INTA Digital |
| description | Mycobacterium bovis causes tuberculosis in a wide variety of mammals, with strong tropism for cattle and eventually humans. P27, also called LprG, is among the proteins involved in the mechanisms of the virulence and persistence of
M. bovis and Mycobacterium tuberculosis. Here, we describe a novel function of P27 in the interaction of M. bovis with its natural host cell, the bovine macrophage. We found that a deletion in the p27-p55 operon impairs the replication of M. bovis in bovine macrophages. Importantly, we show for the first time that M. bovis arrests
phagosome maturation in a process that depends on P27. This effect is P27 specific since complementation with wild-type p27 but not p55 fully restored the wild-type phenotype of the mutant strain; this indicates that P55 plays no important role during the early events of M. bovis infection. In addition, we also showed that the presence of P27 from M. smegmatis decreases the association of LAMP-3 with bead phagosomes, indicating that P27 itself blocks phagosome-lysosome fusion by modulating the traffic machinery in the cell host. |
| format | Artículo |
| id | INTA552 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA5522017-12-22T15:23:31Z Mycobacterium bovis requires P27 (LprG) to arrest phagosome maturation and replicate within bovine macrophages Vazquez, Cristina Lourdes Bianco, María Veronica Blanco, Federico Carlos Forrellad, Marina Andrea Gutierrez, Maximiliano Gabriel Bigi, Fabiana Mycobacterium Bovis Enfermedades de los Animales Tuberculosis Macrofagos Ganado Bovino Animal Diseases Macrophages Cattle LprG Mycobacterium bovis causes tuberculosis in a wide variety of mammals, with strong tropism for cattle and eventually humans. P27, also called LprG, is among the proteins involved in the mechanisms of the virulence and persistence of M. bovis and Mycobacterium tuberculosis. Here, we describe a novel function of P27 in the interaction of M. bovis with its natural host cell, the bovine macrophage. We found that a deletion in the p27-p55 operon impairs the replication of M. bovis in bovine macrophages. Importantly, we show for the first time that M. bovis arrests phagosome maturation in a process that depends on P27. This effect is P27 specific since complementation with wild-type p27 but not p55 fully restored the wild-type phenotype of the mutant strain; this indicates that P55 plays no important role during the early events of M. bovis infection. In addition, we also showed that the presence of P27 from M. smegmatis decreases the association of LAMP-3 with bead phagosomes, indicating that P27 itself blocks phagosome-lysosome fusion by modulating the traffic machinery in the cell host. Instituto de Biotecnología Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Gutierrez, Maximiliano Gabriel. The Francis Crick Institute, Host-Pathogen Interactions in Tuberculosis Laboratory; Reino Unido Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2017-07-04T15:15:21Z 2017-07-04T15:15:21Z 2017-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/552 http://iai.asm.org/content/85/3/e00720-16.full.pdf+html https://doi.org/10.1128/IAI.00720-16 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Infection and immunity 85 (3): e00720-16. (2017 Mar.) |
| spellingShingle | Mycobacterium Bovis Enfermedades de los Animales Tuberculosis Macrofagos Ganado Bovino Animal Diseases Macrophages Cattle LprG Vazquez, Cristina Lourdes Bianco, María Veronica Blanco, Federico Carlos Forrellad, Marina Andrea Gutierrez, Maximiliano Gabriel Bigi, Fabiana Mycobacterium bovis requires P27 (LprG) to arrest phagosome maturation and replicate within bovine macrophages |
| title | Mycobacterium bovis requires P27 (LprG) to arrest phagosome maturation and replicate within bovine macrophages |
| title_full | Mycobacterium bovis requires P27 (LprG) to arrest phagosome maturation and replicate within bovine macrophages |
| title_fullStr | Mycobacterium bovis requires P27 (LprG) to arrest phagosome maturation and replicate within bovine macrophages |
| title_full_unstemmed | Mycobacterium bovis requires P27 (LprG) to arrest phagosome maturation and replicate within bovine macrophages |
| title_short | Mycobacterium bovis requires P27 (LprG) to arrest phagosome maturation and replicate within bovine macrophages |
| title_sort | mycobacterium bovis requires p27 lprg to arrest phagosome maturation and replicate within bovine macrophages |
| topic | Mycobacterium Bovis Enfermedades de los Animales Tuberculosis Macrofagos Ganado Bovino Animal Diseases Macrophages Cattle LprG |
| url | http://hdl.handle.net/20.500.12123/552 http://iai.asm.org/content/85/3/e00720-16.full.pdf+html https://doi.org/10.1128/IAI.00720-16 |
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