Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
Interspecific gene flow is a common phenomenon in Nothofagaceae species; however, the dynamics of introgression in hybrid zones remains largely unknown. We focused on two ecologically and morphologically different Nothofagus species from Patagonia, Nothofagus nervosa and Nothofagus obliqua. In a nat...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer-Verlag Berlin Heidelberg
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/5481 https://link.springer.com/article/10.1007/s11295-017-1132-1 https://doi.org/10.1007/s11295-017-1132-1 |
| _version_ | 1855483630815543296 |
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| author | El Mujtar, Veronica Andrea Sola, Georgina Giselle Aparicio, Alejandro Gabriel Gallo, Leonardo Ariel |
| author_browse | Aparicio, Alejandro Gabriel El Mujtar, Veronica Andrea Gallo, Leonardo Ariel Sola, Georgina Giselle |
| author_facet | El Mujtar, Veronica Andrea Sola, Georgina Giselle Aparicio, Alejandro Gabriel Gallo, Leonardo Ariel |
| author_sort | El Mujtar, Veronica Andrea |
| collection | INTA Digital |
| description | Interspecific gene flow is a common phenomenon in Nothofagaceae species; however, the dynamics of introgression in hybrid zones remains largely unknown. We focused on two ecologically and morphologically different Nothofagus species from Patagonia, Nothofagus nervosa and Nothofagus obliqua. In a natural hybrid zone, we established two plots 280mapart in altitude (ca. 1.9 °C difference in mean temperature), and two subplots which captured microsite variation (abundance and spatial distribution of species and predominance of wind direction). We used intensive sampling of individuals (2055, including adults and regeneration) and molecular genotyping of 6 highly species-specific nuclear microsatellites for the identification and classification of hybrids, based on estimates of ancestry and interclass heterozygosity.
We evaluated the relative contribution of our sampling effects to variation in hybrid incidence and direction of introgression using generalized linear mixed effects models. We determined that introgressive hybridization occurs at a global rate of 7.8% and that variation was mostly explained by plots (frequency at low altitude was approximately twice that found at high altitude), while it was less influenced by subplots. The high altitude plot was dominated by late-generation backcrosses to N. obliqua (asymmetric bimodality), whereas the low altitude plot consisted of intermediate hybrids (unimodality) and showed asymmetry for introgression between subplots. Differences were not detected between adults and regeneration, suggesting early-acting reproductive isolating
barriers. F1 hybrids occur at a global frequency of 3.8%, and are fertile, as the detection of first- and late-generation hybrids indicates. |
| format | Artículo |
| id | INTA5481 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer-Verlag Berlin Heidelberg |
| publisherStr | Springer-Verlag Berlin Heidelberg |
| record_format | dspace |
| spelling | INTA54812019-07-12T11:02:23Z Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests El Mujtar, Veronica Andrea Sola, Georgina Giselle Aparicio, Alejandro Gabriel Gallo, Leonardo Ariel Nothofagus Hibridación Hybridization Forests Bosques Nothofagus Nervosa Nothofagus Obliqua Gradientes Altitudinales Genética Forestal Región Patagónica Interspecific gene flow is a common phenomenon in Nothofagaceae species; however, the dynamics of introgression in hybrid zones remains largely unknown. We focused on two ecologically and morphologically different Nothofagus species from Patagonia, Nothofagus nervosa and Nothofagus obliqua. In a natural hybrid zone, we established two plots 280mapart in altitude (ca. 1.9 °C difference in mean temperature), and two subplots which captured microsite variation (abundance and spatial distribution of species and predominance of wind direction). We used intensive sampling of individuals (2055, including adults and regeneration) and molecular genotyping of 6 highly species-specific nuclear microsatellites for the identification and classification of hybrids, based on estimates of ancestry and interclass heterozygosity. We evaluated the relative contribution of our sampling effects to variation in hybrid incidence and direction of introgression using generalized linear mixed effects models. We determined that introgressive hybridization occurs at a global rate of 7.8% and that variation was mostly explained by plots (frequency at low altitude was approximately twice that found at high altitude), while it was less influenced by subplots. The high altitude plot was dominated by late-generation backcrosses to N. obliqua (asymmetric bimodality), whereas the low altitude plot consisted of intermediate hybrids (unimodality) and showed asymmetry for introgression between subplots. Differences were not detected between adults and regeneration, suggesting early-acting reproductive isolating barriers. F1 hybrids occur at a global frequency of 3.8%, and are fertile, as the detection of first- and late-generation hybrids indicates. Estación Experimental Agropecuaria Bariloche Fil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina Fil: Sola, Georgina Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT-Patagonia Norte; Argentina. Universidad Nacional del Comahue. Cátedra de Ordenación Forestal; Argentina Fil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina 2019-07-12T10:52:01Z 2019-07-12T10:52:01Z 2017-03-29 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5481 https://link.springer.com/article/10.1007/s11295-017-1132-1 1614-2942 1614-2950 https://doi.org/10.1007/s11295-017-1132-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer-Verlag Berlin Heidelberg Tree Genetics & Genomes 13 : 49 (April 2017) |
| spellingShingle | Nothofagus Hibridación Hybridization Forests Bosques Nothofagus Nervosa Nothofagus Obliqua Gradientes Altitudinales Genética Forestal Región Patagónica El Mujtar, Veronica Andrea Sola, Georgina Giselle Aparicio, Alejandro Gabriel Gallo, Leonardo Ariel Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests |
| title | Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests |
| title_full | Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests |
| title_fullStr | Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests |
| title_full_unstemmed | Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests |
| title_short | Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests |
| title_sort | pattern of natural introgression in a nothofagus hybrid zone from south american temperate forests |
| topic | Nothofagus Hibridación Hybridization Forests Bosques Nothofagus Nervosa Nothofagus Obliqua Gradientes Altitudinales Genética Forestal Región Patagónica |
| url | http://hdl.handle.net/20.500.12123/5481 https://link.springer.com/article/10.1007/s11295-017-1132-1 https://doi.org/10.1007/s11295-017-1132-1 |
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