First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa)
Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosis is maintained in nature by chronic renal infection of carrier animals, being rodents and other small mammals the most important reservoirs. Also, significant sources of human infection are domestic animals, such as...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/547 http://ejbio.imedpub.com/first-isolation-of-leptospira-borgpetersenii-from-fetuses-ofwild-boars-sus-scrofa.pdf |
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| author | Brihuega, Bibiana Felicitas Grune Loffler, Sylvia Samartino, Luis Ernesto Romero, Graciela Noemi Auteri, Carmelo Domingo Martinez, Mara Leila |
| author_browse | Auteri, Carmelo Domingo Brihuega, Bibiana Felicitas Grune Loffler, Sylvia Martinez, Mara Leila Romero, Graciela Noemi Samartino, Luis Ernesto |
| author_facet | Brihuega, Bibiana Felicitas Grune Loffler, Sylvia Samartino, Luis Ernesto Romero, Graciela Noemi Auteri, Carmelo Domingo Martinez, Mara Leila |
| author_sort | Brihuega, Bibiana Felicitas |
| collection | INTA Digital |
| description | Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosis is maintained in nature by chronic renal infection of carrier animals, being rodents and other small mammals the most important reservoirs. Also, significant sources of human infection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa) is an introduced species from Europe which is widespread in America and particularly in the Center and South of Argentina. This species is highly valued by hunters in the region and their meat is consumed more often. In many countries of the world (Europe, USA and Australia) studies of seroprevalence have been carried out, the seroreactivity does not mean that the wild boar has clinical symptoms of leptospirosis or that wild boars are maintenance host of this pathogen. However, these animals have been in contact to leptospiras in their environment in the past. This study has the objective of molecular characterization of pathogenic Leptospira sp. isolated strain obtained from fetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wild boar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy was performed of the 4 fetuses and samples of kidneys, liver, spleen and lung were cultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung and kidney of fetuses were processed and analyzed by direct immunofluorescence. Multiple Locus Variable number tandems repeats Analysis (MLVA) was used to characterize the isolated strain. The genetic profile of the isolated pathogenic Leptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovar Castellonis Castellon 3. To the best of our knowledge this is the first isolation of Leptospira spp. from a wild boar fetus. |
| format | Artículo |
| id | INTA547 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA5472018-01-11T17:10:55Z First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa) Brihuega, Bibiana Felicitas Grune Loffler, Sylvia Samartino, Luis Ernesto Romero, Graciela Noemi Auteri, Carmelo Domingo Martinez, Mara Leila Enfermedades de los Animales Sus Scrofa Jabalí Genotipos Wild Boar Genotypes Leptospira borgpetersenii Región Patagónica Leptospirosis is a worldwide zoonose and is endemic in many countries. This zoonosis is maintained in nature by chronic renal infection of carrier animals, being rodents and other small mammals the most important reservoirs. Also, significant sources of human infection are domestic animals, such as livestock and dogs. The wild boar (Sus scrofa) is an introduced species from Europe which is widespread in America and particularly in the Center and South of Argentina. This species is highly valued by hunters in the region and their meat is consumed more often. In many countries of the world (Europe, USA and Australia) studies of seroprevalence have been carried out, the seroreactivity does not mean that the wild boar has clinical symptoms of leptospirosis or that wild boars are maintenance host of this pathogen. However, these animals have been in contact to leptospiras in their environment in the past. This study has the objective of molecular characterization of pathogenic Leptospira sp. isolated strain obtained from fetuses of wild boars from Patagonia Argentina. Four fetuses were aborted from a wild boar (Sus scrofa) in Patagonia, the southern region of Argentina. Necropsy was performed of the 4 fetuses and samples of kidneys, liver, spleen and lung were cultivated in Fletcher and EMJH mediums. Also, samples of liver, spleen, lung and kidney of fetuses were processed and analyzed by direct immunofluorescence. Multiple Locus Variable number tandems repeats Analysis (MLVA) was used to characterize the isolated strain. The genetic profile of the isolated pathogenic Leptospira sp. strain was identical to the profile of Leptospira borgpetersenii serovar Castellonis Castellon 3. To the best of our knowledge this is the first isolation of Leptospira spp. from a wild boar fetus. Instituto de Patobiología Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Auteri, Carmelo Domingo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina 2017-07-03T14:16:33Z 2017-07-03T14:16:33Z 2017-02-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/547 http://ejbio.imedpub.com/first-isolation-of-leptospira-borgpetersenii-from-fetuses-ofwild-boars-sus-scrofa.pdf 1860-3122 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Patagonia (general region) Electronic journal of biology, 13 (1) : 63-66 (2017) |
| spellingShingle | Enfermedades de los Animales Sus Scrofa Jabalí Genotipos Wild Boar Genotypes Leptospira borgpetersenii Región Patagónica Brihuega, Bibiana Felicitas Grune Loffler, Sylvia Samartino, Luis Ernesto Romero, Graciela Noemi Auteri, Carmelo Domingo Martinez, Mara Leila First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa) |
| title | First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa) |
| title_full | First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa) |
| title_fullStr | First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa) |
| title_full_unstemmed | First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa) |
| title_short | First isolation of Leptospira borgpetersenii from Fetuses of Wild boars (Sus scrofa) |
| title_sort | first isolation of leptospira borgpetersenii from fetuses of wild boars sus scrofa |
| topic | Enfermedades de los Animales Sus Scrofa Jabalí Genotipos Wild Boar Genotypes Leptospira borgpetersenii Región Patagónica |
| url | http://hdl.handle.net/20.500.12123/547 http://ejbio.imedpub.com/first-isolation-of-leptospira-borgpetersenii-from-fetuses-ofwild-boars-sus-scrofa.pdf |
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