Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
Viruses of the family Hepadnaviridae are characterized by partially dsDNA circular genomes of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses have a broad host range, which includes humans (hepatitis B virus), other mammals (genus Orthohepadnavirus), and bi...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/5439 https://link.springer.com/article/10.1007/s00705-019-04227-8 https://doi.org/10.1007/s00705-019-04227-8 |
| _version_ | 1855483622641893376 |
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| author | Debat, Humberto Julio Fei Fan Ng, Terry |
| author_browse | Debat, Humberto Julio Fei Fan Ng, Terry |
| author_facet | Debat, Humberto Julio Fei Fan Ng, Terry |
| author_sort | Debat, Humberto Julio |
| collection | INTA Digital |
| description | Viruses of the family Hepadnaviridae are characterized by partially dsDNA circular genomes of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses have a broad host range, which includes humans (hepatitis B virus), other mammals (genus Orthohepadnavirus), and birds (genus Avihepadnavirus). The known host specificity of hepadnaviruses has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. Tibetan frog hepatitis B virus (TFHBV) was recently discovered in a member of the species Nanorana parkeri (family Dicroglossidae) from Tibet. To increase our understanding of hepadnaviruses that infect amphibian hosts, we identified the full-length genome of a divergent strain, TFHBV-Ot, associated with a concave-eared torrent frog (Odorrana tormota, family Ranidae) from China by searching deep-sequencing data. TFHBV-Ot shared a genomic organization and 76.6% overall genome sequence nucleotide identity with the prototype TFHBV associated with N. parkeri (TFHBV-Np). The pairwise amino acid sequence identity between the predicted gene products of TFHBV-Ot and TFHBV-Np ranged between 63.9% and 77.9%. Multiple tissue/organ-specific RNAseq datasets suggested a broad tropism of TFHBV, including muscle, gonads and brain. In addition, we provide information about putative virus-derived small RNAs from an amphibian hepadnavirus. The results presented here expand the known genetic diversity and host range of TFHBV to Ranidae frogs, and warrant an investigation of hepadnaviral infection of amphibian brains. |
| format | Artículo |
| id | INTA5439 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA54392019-07-04T17:10:42Z Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota) Debat, Humberto Julio Fei Fan Ng, Terry Hepatitis Virus de los Animales Rana Genomas Secuencia Nucleotídica Animal Viruses Frogs Genomes Nucleotide Sequence Hepatitis B Rana Tibetana Odorrana Tormota Tibetan Frog Viruses of the family Hepadnaviridae are characterized by partially dsDNA circular genomes of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses have a broad host range, which includes humans (hepatitis B virus), other mammals (genus Orthohepadnavirus), and birds (genus Avihepadnavirus). The known host specificity of hepadnaviruses has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. Tibetan frog hepatitis B virus (TFHBV) was recently discovered in a member of the species Nanorana parkeri (family Dicroglossidae) from Tibet. To increase our understanding of hepadnaviruses that infect amphibian hosts, we identified the full-length genome of a divergent strain, TFHBV-Ot, associated with a concave-eared torrent frog (Odorrana tormota, family Ranidae) from China by searching deep-sequencing data. TFHBV-Ot shared a genomic organization and 76.6% overall genome sequence nucleotide identity with the prototype TFHBV associated with N. parkeri (TFHBV-Np). The pairwise amino acid sequence identity between the predicted gene products of TFHBV-Ot and TFHBV-Np ranged between 63.9% and 77.9%. Multiple tissue/organ-specific RNAseq datasets suggested a broad tropism of TFHBV, including muscle, gonads and brain. In addition, we provide information about putative virus-derived small RNAs from an amphibian hepadnavirus. The results presented here expand the known genetic diversity and host range of TFHBV to Ranidae frogs, and warrant an investigation of hepadnaviral infection of amphibian brains. Instituto de Patología Vegetal Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fei Fan Ng, Terry. University of Georgia. College of Veterinary Medicine; Estados Unidos 2019-07-04T14:55:45Z 2019-07-04T14:55:45Z 2019-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5439 https://link.springer.com/article/10.1007/s00705-019-04227-8 0304-8608 1432-8798 (Online) https://doi.org/10.1007/s00705-019-04227-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Archives of virology 164 (6) : 1727–1732. (June 2019) |
| spellingShingle | Hepatitis Virus de los Animales Rana Genomas Secuencia Nucleotídica Animal Viruses Frogs Genomes Nucleotide Sequence Hepatitis B Rana Tibetana Odorrana Tormota Tibetan Frog Debat, Humberto Julio Fei Fan Ng, Terry Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota) |
| title | Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota) |
| title_full | Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota) |
| title_fullStr | Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota) |
| title_full_unstemmed | Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota) |
| title_short | Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota) |
| title_sort | complete genome sequence of a divergent strain of tibetan frog hepatitis b virus associated with a concave eared torrent frog odorrana tormota |
| topic | Hepatitis Virus de los Animales Rana Genomas Secuencia Nucleotídica Animal Viruses Frogs Genomes Nucleotide Sequence Hepatitis B Rana Tibetana Odorrana Tormota Tibetan Frog |
| url | http://hdl.handle.net/20.500.12123/5439 https://link.springer.com/article/10.1007/s00705-019-04227-8 https://doi.org/10.1007/s00705-019-04227-8 |
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