Olfactory attraction of the larval parasitoid, Hyposoter horticola, to plants infested with eggs of the host butterfly, Melitaea cinxia

Parasitoids locate inconspicuous hosts in a heterogeneous habitat using plant volatiles, some of which are induced by the hosts. Hyposoter horticola Gravenhost (Hymenoptera: Ichneumonidae) is a parasitoid of the Glanville fritillary butterfly Melitaea cinxia L. (Lepidoptera: Nymphalidae). Melitaea c...

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Main Authors: Castelo, Marcela Karina, Nouhuys, Saskya van, Corley, Juan Carlos
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Entomological Society of America 2019
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/5426
https://academic.oup.com/jinsectscience/article/10/1/53/837856
https://doi.org/10.1673/031.010.5301
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author Castelo, Marcela Karina
Nouhuys, Saskya van
Corley, Juan Carlos
author_browse Castelo, Marcela Karina
Corley, Juan Carlos
Nouhuys, Saskya van
author_facet Castelo, Marcela Karina
Nouhuys, Saskya van
Corley, Juan Carlos
author_sort Castelo, Marcela Karina
collection INTA Digital
description Parasitoids locate inconspicuous hosts in a heterogeneous habitat using plant volatiles, some of which are induced by the hosts. Hyposoter horticola Gravenhost (Hymenoptera: Ichneumonidae) is a parasitoid of the Glanville fritillary butterfly Melitaea cinxia L. (Lepidoptera: Nymphalidae). Melitaea cinxia lays eggs in clusters on leaves of Plantago lanceolata L. (Lamiales: Plantaginaceae) and Veronica spicata L. (Lamiales: Plantaginaceae). The parasitoid oviposits into host larvae that have not yet hatched from the egg. Thus, though H. horticola is a parasitoid of Melitaea cinxia larvae, it must find host eggs on plants that have not been fed on by the larvae. Using a Y-tube olfactometer, the response of H. horticola to odors of Melitaea cinxia and extracts of the attacked plant species were tested. Three week-old eggs (near hatching) were attractive to young H. horticola, but one week-old eggs were attractive only to old or experiencedH. horticola. Melitaea cinxia larvae were not attractive. A water extract of P. lanceolata was attractive, but ethanol or hexane extracts were not. None of the extracts of V. spicata were attractive. Leaves of V. spicata were attractive only if harboring eggs, but P. lanceolata leaves with eggs were not. Free flying H. horticola in a large outdoor enclosure were presented with host and plant cues. As in the olfactometer, V. spicata was attractive only when eggs were on it, and P. lanceolata was somewhat attractive with or without eggs. This study shows for the first time that a parasitoid of larvae uses egg volatiles or oviposition-induced plant volatiles, to f0ind host larvae, and that Melitaea cinxia eggs or traces of oviposition induce the production of these volatiles by the plant. Based on the results, and given the natural distribution of the plants and M cinxia eggs, parasitism of Melitaea cinxia eggs on P. lanceolata would be expected to be low. Instead, under natural conditions, a fraction of the eggs in virtually all egg clusters are parasitized on both plant species. The mismatch between the experimental results and the natural pattern of host-parasitoid interactions is discussed in terms of the expected coupling foraging cues with foraging success.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA54262024-07-23T14:26:52Z Olfactory attraction of the larval parasitoid, Hyposoter horticola, to plants infested with eggs of the host butterfly, Melitaea cinxia Castelo, Marcela Karina Nouhuys, Saskya van Corley, Juan Carlos Parasitoides Hyposoter Control de Plagas Huéspedes Olfacción Parasitoids Pest Control Hosts Olfaction Hyposoter horticola Melitaea cinxia Parasitoids locate inconspicuous hosts in a heterogeneous habitat using plant volatiles, some of which are induced by the hosts. Hyposoter horticola Gravenhost (Hymenoptera: Ichneumonidae) is a parasitoid of the Glanville fritillary butterfly Melitaea cinxia L. (Lepidoptera: Nymphalidae). Melitaea cinxia lays eggs in clusters on leaves of Plantago lanceolata L. (Lamiales: Plantaginaceae) and Veronica spicata L. (Lamiales: Plantaginaceae). The parasitoid oviposits into host larvae that have not yet hatched from the egg. Thus, though H. horticola is a parasitoid of Melitaea cinxia larvae, it must find host eggs on plants that have not been fed on by the larvae. Using a Y-tube olfactometer, the response of H. horticola to odors of Melitaea cinxia and extracts of the attacked plant species were tested. Three week-old eggs (near hatching) were attractive to young H. horticola, but one week-old eggs were attractive only to old or experiencedH. horticola. Melitaea cinxia larvae were not attractive. A water extract of P. lanceolata was attractive, but ethanol or hexane extracts were not. None of the extracts of V. spicata were attractive. Leaves of V. spicata were attractive only if harboring eggs, but P. lanceolata leaves with eggs were not. Free flying H. horticola in a large outdoor enclosure were presented with host and plant cues. As in the olfactometer, V. spicata was attractive only when eggs were on it, and P. lanceolata was somewhat attractive with or without eggs. This study shows for the first time that a parasitoid of larvae uses egg volatiles or oviposition-induced plant volatiles, to f0ind host larvae, and that Melitaea cinxia eggs or traces of oviposition induce the production of these volatiles by the plant. Based on the results, and given the natural distribution of the plants and M cinxia eggs, parasitism of Melitaea cinxia eggs on P. lanceolata would be expected to be low. Instead, under natural conditions, a fraction of the eggs in virtually all egg clusters are parasitized on both plant species. The mismatch between the experimental results and the natural pattern of host-parasitoid interactions is discussed in terms of the expected coupling foraging cues with foraging success. EEA Bariloche Fil: Castelo, Marcela Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Nouhuys, Saskya van. University of Helsinski. Department of Biological and Environmental Sciences. Division of Population Biology; Finlandia. Cornell University. Department of Ecology and Evolutionary Biology; Estados Unidos Fil: Corley, Juan Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Ecología de Poblaciones de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-07-03T13:33:22Z 2019-07-03T13:33:22Z 2010-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5426 https://academic.oup.com/jinsectscience/article/10/1/53/837856 1536-2442 https://doi.org/10.1673/031.010.5301 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Entomological Society of America Journal of Insect Science 10 (1) : 53. (2010)
spellingShingle Parasitoides
Hyposoter
Control de Plagas
Huéspedes
Olfacción
Parasitoids
Pest Control
Hosts
Olfaction
Hyposoter horticola
Melitaea cinxia
Castelo, Marcela Karina
Nouhuys, Saskya van
Corley, Juan Carlos
Olfactory attraction of the larval parasitoid, Hyposoter horticola, to plants infested with eggs of the host butterfly, Melitaea cinxia
title Olfactory attraction of the larval parasitoid, Hyposoter horticola, to plants infested with eggs of the host butterfly, Melitaea cinxia
title_full Olfactory attraction of the larval parasitoid, Hyposoter horticola, to plants infested with eggs of the host butterfly, Melitaea cinxia
title_fullStr Olfactory attraction of the larval parasitoid, Hyposoter horticola, to plants infested with eggs of the host butterfly, Melitaea cinxia
title_full_unstemmed Olfactory attraction of the larval parasitoid, Hyposoter horticola, to plants infested with eggs of the host butterfly, Melitaea cinxia
title_short Olfactory attraction of the larval parasitoid, Hyposoter horticola, to plants infested with eggs of the host butterfly, Melitaea cinxia
title_sort olfactory attraction of the larval parasitoid hyposoter horticola to plants infested with eggs of the host butterfly melitaea cinxia
topic Parasitoides
Hyposoter
Control de Plagas
Huéspedes
Olfacción
Parasitoids
Pest Control
Hosts
Olfaction
Hyposoter horticola
Melitaea cinxia
url http://hdl.handle.net/20.500.12123/5426
https://academic.oup.com/jinsectscience/article/10/1/53/837856
https://doi.org/10.1673/031.010.5301
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AT corleyjuancarlos olfactoryattractionofthelarvalparasitoidhyposoterhorticolatoplantsinfestedwitheggsofthehostbutterflymelitaeacinxia