Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
Anthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness of its control using conventional insecticide applications and biocontrol techni...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Entomological Society of America
2019
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| Materias: | |
| Acceso en línea: | https://academic.oup.com/jinsectscience/article/14/1/219/2381789 http://hdl.handle.net/20.500.12123/5422 https://doi.org/10.1093/jisesa/ieu081 |
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| author | Salvador, Ricardo Perez Principi, Ricardo Dario Berretta, Marcelo Facundo Fernandez, Paula Del Carmen Paniego, Norma Beatriz Sciocco, Alicia Ines Hopp, Horacio Esteban |
| author_browse | Berretta, Marcelo Facundo Fernandez, Paula Del Carmen Hopp, Horacio Esteban Paniego, Norma Beatriz Perez Principi, Ricardo Dario Salvador, Ricardo Sciocco, Alicia Ines |
| author_facet | Salvador, Ricardo Perez Principi, Ricardo Dario Berretta, Marcelo Facundo Fernandez, Paula Del Carmen Paniego, Norma Beatriz Sciocco, Alicia Ines Hopp, Horacio Esteban |
| author_sort | Salvador, Ricardo |
| collection | INTA Digital |
| description | Anthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness of its control using conventional insecticide applications and biocontrol techniques. In spite of its importance, little is known about its genome sequence and, more important, its specific expression in key organs like the midgut. Total mRNA isolated from larval midguts was used for pyrosequencing. Sequence reads were assembled and annotated to generate a unigene data set. In total, 400,000 reads from A. grandis midgut with an average length of 237 bp were assembled and combined into 20,915 contigs. The assembled reads fell into 6,621 genes models. BlastX search using the NCBI-NR database showed that 3,006 unigenes had significant matches to known sequences. Gene Ontology (GO) mapping analysis evidenced that A. grandis is able to transcripts coding for proteins involved in catalytic processing of macromolecules that allows its adaptation to very different feeding source scenarios. Furthermore, transcripts encoding for proteins involved in detoxification mechanisms such as p450 genes, glutathione-S-transferase, and carboxylesterases are also expressed. This is the first report of a transcriptomic study in A. grandis and the largest set of sequence data reported for this species. These data are valuable resources to expand the knowledge of this insect group and could be used in the design of new control strategies based in molecular information. |
| format | Artículo |
| id | INTA5422 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Entomological Society of America |
| publisherStr | Entomological Society of America |
| record_format | dspace |
| spelling | INTA54222019-07-03T12:38:41Z Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae) Salvador, Ricardo Perez Principi, Ricardo Dario Berretta, Marcelo Facundo Fernandez, Paula Del Carmen Paniego, Norma Beatriz Sciocco, Alicia Ines Hopp, Horacio Esteban Coleoptera Anthonomus grandis Plagas de Plantas Control de Plagas Algodón ARN Pests of Plants Pest Control Cotton RNA Anthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness of its control using conventional insecticide applications and biocontrol techniques. In spite of its importance, little is known about its genome sequence and, more important, its specific expression in key organs like the midgut. Total mRNA isolated from larval midguts was used for pyrosequencing. Sequence reads were assembled and annotated to generate a unigene data set. In total, 400,000 reads from A. grandis midgut with an average length of 237 bp were assembled and combined into 20,915 contigs. The assembled reads fell into 6,621 genes models. BlastX search using the NCBI-NR database showed that 3,006 unigenes had significant matches to known sequences. Gene Ontology (GO) mapping analysis evidenced that A. grandis is able to transcripts coding for proteins involved in catalytic processing of macromolecules that allows its adaptation to very different feeding source scenarios. Furthermore, transcripts encoding for proteins involved in detoxification mechanisms such as p450 genes, glutathione-S-transferase, and carboxylesterases are also expressed. This is the first report of a transcriptomic study in A. grandis and the largest set of sequence data reported for this species. These data are valuable resources to expand the knowledge of this insect group and could be used in the design of new control strategies based in molecular information. Instituto de Microbiología y Zoología Agrícola Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Perez Principi, Ricardo Dario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-07-03T12:37:10Z 2019-07-03T12:37:10Z 2014-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/jinsectscience/article/14/1/219/2381789 http://hdl.handle.net/20.500.12123/5422 1536-2442 https://doi.org/10.1093/jisesa/ieu081 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Entomological Society of America Journal of Insect Science 14 (1) : 219. (2014) |
| spellingShingle | Coleoptera Anthonomus grandis Plagas de Plantas Control de Plagas Algodón ARN Pests of Plants Pest Control Cotton RNA Salvador, Ricardo Perez Principi, Ricardo Dario Berretta, Marcelo Facundo Fernandez, Paula Del Carmen Paniego, Norma Beatriz Sciocco, Alicia Ines Hopp, Horacio Esteban Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae) |
| title | Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae) |
| title_full | Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae) |
| title_fullStr | Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae) |
| title_full_unstemmed | Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae) |
| title_short | Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae) |
| title_sort | transcriptomic survey of the midgut of anthonomus grandis coleoptera curculionidae |
| topic | Coleoptera Anthonomus grandis Plagas de Plantas Control de Plagas Algodón ARN Pests of Plants Pest Control Cotton RNA |
| url | https://academic.oup.com/jinsectscience/article/14/1/219/2381789 http://hdl.handle.net/20.500.12123/5422 https://doi.org/10.1093/jisesa/ieu081 |
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