Physiological and molecular mechanisms underlying the integration of light and temperature cues in Arabidopsis thaliana seeds
The relief of dormancy and the promotion of seed germination are of extreme importance for a successful seedling establishment. Although alternating temperatures and light are signals promoting the relief of seed dormancy, the underlying mechanisms of their interaction in seeds are scarcely known. B...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/5369 https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13076 https://doi.org/10.1111/pce.13076 |
| _version_ | 1855035485861183488 |
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| author | Arana, María Veronica Tognacca, Rocío Soledad Estravis Barcala, Maximiliano Sanchez, Rodolfo Augusto Botto, Javier Francisco |
| author_browse | Arana, María Veronica Botto, Javier Francisco Estravis Barcala, Maximiliano Sanchez, Rodolfo Augusto Tognacca, Rocío Soledad |
| author_facet | Arana, María Veronica Tognacca, Rocío Soledad Estravis Barcala, Maximiliano Sanchez, Rodolfo Augusto Botto, Javier Francisco |
| author_sort | Arana, María Veronica |
| collection | INTA Digital |
| description | The relief of dormancy and the promotion of seed germination are of extreme importance for a successful seedling establishment. Although alternating temperatures and light are signals promoting the relief of seed dormancy, the underlying mechanisms of their interaction in seeds are scarcely known. By exposing imbibed Arabidopsis thaliana dormant seeds to two‐day temperature cycles previous of a red light pulse, we demonstrate that the germination mediated by phytochrome B requires the presence of functional PSEUDO‐RESPONSE REGULATOR 7 (PRR7) and TIMING OF CAB EXPRESSION 1 (TOC1) alleles. In addition, daily cycles of alternating temperatures in darkness reduce the protein levels of DELAY OF GERMINATION 1 (DOG1), allowing the expression of TOC1 to induce seed germination. Our results suggest a functional role for some components of the circadian clock related with the action of DOG1 for the integration of alternating temperatures and light signals in the relief of seed dormancy. The synchronization of germination by the synergic action of light and temperature through the activity of circadian clock might have ecological and adaptive consequences. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5369 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA53692019-06-25T12:56:47Z Physiological and molecular mechanisms underlying the integration of light and temperature cues in Arabidopsis thaliana seeds Arana, María Veronica Tognacca, Rocío Soledad Estravis Barcala, Maximiliano Sanchez, Rodolfo Augusto Botto, Javier Francisco Dormición Interruptores de Latencia Germinación Semillas Dormancy Dormancy Breakers Germination Seed Arabidopsis thaliana Latencia Latencia de Semillas Reloj Circadiano The relief of dormancy and the promotion of seed germination are of extreme importance for a successful seedling establishment. Although alternating temperatures and light are signals promoting the relief of seed dormancy, the underlying mechanisms of their interaction in seeds are scarcely known. By exposing imbibed Arabidopsis thaliana dormant seeds to two‐day temperature cycles previous of a red light pulse, we demonstrate that the germination mediated by phytochrome B requires the presence of functional PSEUDO‐RESPONSE REGULATOR 7 (PRR7) and TIMING OF CAB EXPRESSION 1 (TOC1) alleles. In addition, daily cycles of alternating temperatures in darkness reduce the protein levels of DELAY OF GERMINATION 1 (DOG1), allowing the expression of TOC1 to induce seed germination. Our results suggest a functional role for some components of the circadian clock related with the action of DOG1 for the integration of alternating temperatures and light signals in the relief of seed dormancy. The synchronization of germination by the synergic action of light and temperature through the activity of circadian clock might have ecological and adaptive consequences. Estación Experimental Agropecuaria Bariloche Fil: Arana, María Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Tognacca, Rocío Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Estravis Barcala, Maximiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Sanchez, Rodolfo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Botto, Javier Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina 2019-06-25T12:47:06Z 2019-06-25T12:47:06Z 2017-09-23 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5369 https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13076 1365-3040 https://doi.org/10.1111/pce.13076 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant, Cell & Environment 40 (12) : 3113-3121. (December 2017) |
| spellingShingle | Dormición Interruptores de Latencia Germinación Semillas Dormancy Dormancy Breakers Germination Seed Arabidopsis thaliana Latencia Latencia de Semillas Reloj Circadiano Arana, María Veronica Tognacca, Rocío Soledad Estravis Barcala, Maximiliano Sanchez, Rodolfo Augusto Botto, Javier Francisco Physiological and molecular mechanisms underlying the integration of light and temperature cues in Arabidopsis thaliana seeds |
| title | Physiological and molecular mechanisms underlying the integration of light and temperature cues in Arabidopsis thaliana seeds |
| title_full | Physiological and molecular mechanisms underlying the integration of light and temperature cues in Arabidopsis thaliana seeds |
| title_fullStr | Physiological and molecular mechanisms underlying the integration of light and temperature cues in Arabidopsis thaliana seeds |
| title_full_unstemmed | Physiological and molecular mechanisms underlying the integration of light and temperature cues in Arabidopsis thaliana seeds |
| title_short | Physiological and molecular mechanisms underlying the integration of light and temperature cues in Arabidopsis thaliana seeds |
| title_sort | physiological and molecular mechanisms underlying the integration of light and temperature cues in arabidopsis thaliana seeds |
| topic | Dormición Interruptores de Latencia Germinación Semillas Dormancy Dormancy Breakers Germination Seed Arabidopsis thaliana Latencia Latencia de Semillas Reloj Circadiano |
| url | http://hdl.handle.net/20.500.12123/5369 https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13076 https://doi.org/10.1111/pce.13076 |
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