Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrade...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Acceso en línea: | https://www.apsnet.org/publications/mpmi/2010/April/Pages/23_4_394.aspx http://hdl.handle.net/20.500.12123/5317 http://dx.doi.org/10.1094/MPMI-23-4-0394 |
| _version_ | 1855483600634380288 |
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| author | Sanchez, Gerardo Gerhardt, Nadia Siciliano, Florencia Vojnov, Adrián Malcuit, Isabelle Marano, María Rosa |
| author_browse | Gerhardt, Nadia Malcuit, Isabelle Marano, María Rosa Sanchez, Gerardo Siciliano, Florencia Vojnov, Adrián |
| author_facet | Sanchez, Gerardo Gerhardt, Nadia Siciliano, Florencia Vojnov, Adrián Malcuit, Isabelle Marano, María Rosa |
| author_sort | Sanchez, Gerardo |
| collection | INTA Digital |
| description | To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species--generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX. |
| format | Artículo |
| id | INTA5317 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | INTA53172019-06-14T11:37:38Z Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum Sanchez, Gerardo Gerhardt, Nadia Siciliano, Florencia Vojnov, Adrián Malcuit, Isabelle Marano, María Rosa Papa Solanum tuberosum Ácido Salicílico Potatoes Potato Virus A Salicylic Acid To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species--generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX. EEA San Pedro Fil: Sánchez, Gerardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Fil: Gerhardt, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Fil: Siciliano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Vojnov, Adrián. Fundación Pablo Cassará; Argentina Fil: Malcuit, Isabelle. Algentech Limited; Reino Unido. Institute of Food Research; Reino Unido Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2019-06-14T11:31:43Z 2019-06-14T11:31:43Z 2010 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.apsnet.org/publications/mpmi/2010/April/Pages/23_4_394.aspx http://hdl.handle.net/20.500.12123/5317 0894-0282 1943-7706 http://dx.doi.org/10.1094/MPMI-23-4-0394 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Molecular Plant-Microbe Interactions 23 (4) : 394-405. (2010) |
| spellingShingle | Papa Solanum tuberosum Ácido Salicílico Potatoes Potato Virus A Salicylic Acid Sanchez, Gerardo Gerhardt, Nadia Siciliano, Florencia Vojnov, Adrián Malcuit, Isabelle Marano, María Rosa Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum |
| title | Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum |
| title_full | Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum |
| title_fullStr | Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum |
| title_full_unstemmed | Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum |
| title_short | Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum |
| title_sort | salicylic acid is involved in the nb mediated defense responses to potato virus x in solanum tuberosum |
| topic | Papa Solanum tuberosum Ácido Salicílico Potatoes Potato Virus A Salicylic Acid |
| url | https://www.apsnet.org/publications/mpmi/2010/April/Pages/23_4_394.aspx http://hdl.handle.net/20.500.12123/5317 http://dx.doi.org/10.1094/MPMI-23-4-0394 |
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