Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
Fruit from rosaceous species collectively display a great variety of flavors and textures as well as a generally high content of nutritionally beneficial metabolites. However, relatively little analysis of metabolic networks in rosaceous fruit has been reported. Among rosaceous species, peach (Prunu...
| Main Authors: | , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://www.plantphysiol.org/content/157/4/1696 http://hdl.handle.net/20.500.12123/5310 https://doi.org/10.1104/pp.111.186064 |
| _version_ | 1855035475876642816 |
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| author | Lombrado, Verónica A. Osorio, Sonia Borsani, Julia Lauxmann, Martín Alexander Bustamante, Claudia Anabel Budde, Claudio Olaf Andreo, Carlos Santiago Lara, María Valeria Fernie, Alisdair R. Drincovich, María Fabiana |
| author_browse | Andreo, Carlos Santiago Borsani, Julia Budde, Claudio Olaf Bustamante, Claudia Anabel Drincovich, María Fabiana Fernie, Alisdair R. Lara, María Valeria Lauxmann, Martín Alexander Lombrado, Verónica A. Osorio, Sonia |
| author_facet | Lombrado, Verónica A. Osorio, Sonia Borsani, Julia Lauxmann, Martín Alexander Bustamante, Claudia Anabel Budde, Claudio Olaf Andreo, Carlos Santiago Lara, María Valeria Fernie, Alisdair R. Drincovich, María Fabiana |
| author_sort | Lombrado, Verónica A. |
| collection | INTA Digital |
| description | Fruit from rosaceous species collectively display a great variety of flavors and textures as well as a generally high content of nutritionally beneficial metabolites. However, relatively little analysis of metabolic networks in rosaceous fruit has been reported. Among rosaceous species, peach (Prunus persica) has stone fruits composed of a juicy mesocarp and lignified endocarp. Here, peach mesocarp metabolic networks were studied across development using metabolomics and analysis of key regulatory enzymes. Principal component analysis of peach metabolic composition revealed clear metabolic shifts from early through late development stages and subsequently during postharvest ripening. Early developmental stages were characterized by a substantial decrease in protein abundance and high levels of bioactive polyphenols and amino acids, which are substrates for the phenylpropanoid and lignin pathways during stone hardening. Sucrose levels showed a large increase during development, reflecting translocation from the leaf, while the importance of galactinol and raffinose is also inferred. Our study further suggests that posttranscriptional mechanisms are key for metabolic regulation at early stages. In contrast to early developmental stages, a decrease in amino acid levels is coupled to an induction of transcripts encoding amino acid and organic acid catabolic enzymes during ripening. These data are consistent with the mobilization of amino acids to support respiration. In addition, sucrose cycling, suggested by the parallel increase of transcripts encoding sucrose degradative and synthetic enzymes, appears to operate during postharvest ripening. When taken together, these data highlight singular metabolic programs for peach development and may allow the identification of key factors related to agronomic traits of this important crop species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5310 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | INTA53102019-06-13T13:42:08Z Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage Lombrado, Verónica A. Osorio, Sonia Borsani, Julia Lauxmann, Martín Alexander Bustamante, Claudia Anabel Budde, Claudio Olaf Andreo, Carlos Santiago Lara, María Valeria Fernie, Alisdair R. Drincovich, María Fabiana Durazno Desarrollo Biológico Crecimiento Fruto Metabolitos Maduramiento Metabolismo Peaches Biological Development Growth Fruit Metabolites Ripening Metabolism Desarrollo del Fruto Crecimiento del Fruto Perfil Metabólico Fruit Development Fruit Growth Metabolic Profiling Fruit from rosaceous species collectively display a great variety of flavors and textures as well as a generally high content of nutritionally beneficial metabolites. However, relatively little analysis of metabolic networks in rosaceous fruit has been reported. Among rosaceous species, peach (Prunus persica) has stone fruits composed of a juicy mesocarp and lignified endocarp. Here, peach mesocarp metabolic networks were studied across development using metabolomics and analysis of key regulatory enzymes. Principal component analysis of peach metabolic composition revealed clear metabolic shifts from early through late development stages and subsequently during postharvest ripening. Early developmental stages were characterized by a substantial decrease in protein abundance and high levels of bioactive polyphenols and amino acids, which are substrates for the phenylpropanoid and lignin pathways during stone hardening. Sucrose levels showed a large increase during development, reflecting translocation from the leaf, while the importance of galactinol and raffinose is also inferred. Our study further suggests that posttranscriptional mechanisms are key for metabolic regulation at early stages. In contrast to early developmental stages, a decrease in amino acid levels is coupled to an induction of transcripts encoding amino acid and organic acid catabolic enzymes during ripening. These data are consistent with the mobilization of amino acids to support respiration. In addition, sucrose cycling, suggested by the parallel increase of transcripts encoding sucrose degradative and synthetic enzymes, appears to operate during postharvest ripening. When taken together, these data highlight singular metabolic programs for peach development and may allow the identification of key factors related to agronomic traits of this important crop species. EEA San Pedro Fil: Lombardo, Verónica A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Osorio, Sonia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania Fil: Borsani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Fernie, Alisdair R. Max-Planck-Institute für Molekulare Pflanzenphysiologie; Alemania Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2019-06-13T13:34:11Z 2019-06-13T13:34:11Z 2011-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://www.plantphysiol.org/content/157/4/1696 http://hdl.handle.net/20.500.12123/5310 0032-0889 1532-2548e https://doi.org/10.1104/pp.111.186064 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant Physiology 157 (4) : 1696-1710 (dec. 2011). |
| spellingShingle | Durazno Desarrollo Biológico Crecimiento Fruto Metabolitos Maduramiento Metabolismo Peaches Biological Development Growth Fruit Metabolites Ripening Metabolism Desarrollo del Fruto Crecimiento del Fruto Perfil Metabólico Fruit Development Fruit Growth Metabolic Profiling Lombrado, Verónica A. Osorio, Sonia Borsani, Julia Lauxmann, Martín Alexander Bustamante, Claudia Anabel Budde, Claudio Olaf Andreo, Carlos Santiago Lara, María Valeria Fernie, Alisdair R. Drincovich, María Fabiana Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage |
| title | Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage |
| title_full | Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage |
| title_fullStr | Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage |
| title_full_unstemmed | Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage |
| title_short | Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage |
| title_sort | metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage |
| topic | Durazno Desarrollo Biológico Crecimiento Fruto Metabolitos Maduramiento Metabolismo Peaches Biological Development Growth Fruit Metabolites Ripening Metabolism Desarrollo del Fruto Crecimiento del Fruto Perfil Metabólico Fruit Development Fruit Growth Metabolic Profiling |
| url | http://www.plantphysiol.org/content/157/4/1696 http://hdl.handle.net/20.500.12123/5310 https://doi.org/10.1104/pp.111.186064 |
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