In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions
The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 in...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0042682212002115 http://hdl.handle.net/20.500.12123/5308 https://doi.org/10.1016/j.virol.2012.04.016 |
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| author | Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Llauger, Gabriela Alfonso, Victoria Taboga, Oscar Alberto Del Vas, Mariana |
| author_browse | Alfonso, Victoria Del Vas, Mariana Llauger, Gabriela Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Taboga, Oscar Alberto |
| author_facet | Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Llauger, Gabriela Alfonso, Victoria Taboga, Oscar Alberto Del Vas, Mariana |
| author_sort | Maroniche, Guillermo Andrés |
| collection | INTA Digital |
| description | The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also entered the nucleus and therefore, along with P5-2, could function as regulators of host gene expression. P6 located in the cytoplasm and in perinuclear cloud-like inclusions, was driven to P9-1 viroplasm-like structures and co-localized with P7-2, P10 and α-tubulin, suggesting its involvement in viroplasm formation and viral intracellular movement. Finally, P9-2 was N-glycosylated and located at the plasma membrane in association with filopodia-like protrusions containing actin, suggesting a possible role in virus cell-to-cell movement and spread. |
| format | Artículo |
| id | INTA5308 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA53082019-06-13T13:02:09Z In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Llauger, Gabriela Alfonso, Victoria Taboga, Oscar Alberto Del Vas, Mariana Virus de las Plantas Enfermedades de las Plantas Proteínas Experimentación in Vivo Plant Viruses Plant Diseases Proteins In Vivo Experimentation Virus del Mal de Río Cuarto Mal de Río Cuarto virus The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also entered the nucleus and therefore, along with P5-2, could function as regulators of host gene expression. P6 located in the cytoplasm and in perinuclear cloud-like inclusions, was driven to P9-1 viroplasm-like structures and co-localized with P7-2, P10 and α-tubulin, suggesting its involvement in viroplasm formation and viral intracellular movement. Finally, P9-2 was N-glycosylated and located at the plasma membrane in association with filopodia-like protrusions containing actin, suggesting a possible role in virus cell-to-cell movement and spread. Instituto de Biotecnología Fil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Mongelli, Vanesa Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-06-13T13:00:45Z 2019-06-13T13:00:45Z 2012-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0042682212002115 http://hdl.handle.net/20.500.12123/5308 0042-6822 https://doi.org/10.1016/j.virol.2012.04.016 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Virology 430 (2) : 81-89 (September 2012) |
| spellingShingle | Virus de las Plantas Enfermedades de las Plantas Proteínas Experimentación in Vivo Plant Viruses Plant Diseases Proteins In Vivo Experimentation Virus del Mal de Río Cuarto Mal de Río Cuarto virus Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Llauger, Gabriela Alfonso, Victoria Taboga, Oscar Alberto Del Vas, Mariana In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions |
| title | In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions |
| title_full | In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions |
| title_fullStr | In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions |
| title_full_unstemmed | In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions |
| title_short | In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions |
| title_sort | in vivo subcellular localization of mal de rio cuarto virus mrcv non structural proteins in insect cells reveals their putative functions |
| topic | Virus de las Plantas Enfermedades de las Plantas Proteínas Experimentación in Vivo Plant Viruses Plant Diseases Proteins In Vivo Experimentation Virus del Mal de Río Cuarto Mal de Río Cuarto virus |
| url | https://www.sciencedirect.com/science/article/pii/S0042682212002115 http://hdl.handle.net/20.500.12123/5308 https://doi.org/10.1016/j.virol.2012.04.016 |
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