A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies
Foot and mouth disease is caused by a non-enveloped virus (FMDV), which disposes several antigenic sites at the surface of their capsid proteins. The most relevant and immunodominant antigenic site of FMDV (site A or AnSA) includes a key virus–cell interaction element (RGD motif) located in the Vira...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0022175915300090 http://hdl.handle.net/20.500.12123/5301 https://doi.org/10.1016/j.jim.2015.06.008 |
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| author | Marrero Diaz De Villegas, Rubén Rodríguez Limardo, Ramiro Carrillo, Elisa Cristina Konig, Guido Alberto Turjanski, Adrián G. |
| author_browse | Carrillo, Elisa Cristina Konig, Guido Alberto Marrero Diaz De Villegas, Rubén Rodríguez Limardo, Ramiro Turjanski, Adrián G. |
| author_facet | Marrero Diaz De Villegas, Rubén Rodríguez Limardo, Ramiro Carrillo, Elisa Cristina Konig, Guido Alberto Turjanski, Adrián G. |
| author_sort | Marrero Diaz De Villegas, Rubén |
| collection | INTA Digital |
| description | Foot and mouth disease is caused by a non-enveloped virus (FMDV), which disposes several antigenic sites at the surface of their capsid proteins. The most relevant and immunodominant antigenic site of FMDV (site A or AnSA) includes a key virus–cell interaction element (RGD motif) located in the Viral Protein 1 (VP1), more precisely at the GH loop. AnSA includes a set of overlapped and mainly linear epitopes, which are the main targets of the humoral immune response. Taking advantage over specific structural features of the GH loop, we have evaluated the influence of every amino acid residue at AnSA in the interaction with 2 neutralizing antibodies by molecular modeling techniques. Additionally, we constructed diverse interaction complexes with multiple site A mutants and discussed about the structural influence of amino acidic insertions in such relevant antigenic site of FMDV. Our approach is in agreement with previous ELISA experiments and allows the understanding of how FMDV mutations may alter the interaction with different antibodies, as we can estimate the contribution of each amino acid to the interaction. Overall, our work contributes to the development of specific vaccination strategies for FMD control. |
| format | Artículo |
| id | INTA5301 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA53012019-06-12T12:59:42Z A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies Marrero Diaz De Villegas, Rubén Rodríguez Limardo, Ramiro Carrillo, Elisa Cristina Konig, Guido Alberto Turjanski, Adrián G. Fiebre Aftosa Enfermedades de los Animales Virus Fiebre Aftosa Anticuerpos Monoclonales Bioinformática Foot and Mouth Disease Animal Diseases Aphthovirus Monoclonal Antibodies Bioinformatics Foot and mouth disease is caused by a non-enveloped virus (FMDV), which disposes several antigenic sites at the surface of their capsid proteins. The most relevant and immunodominant antigenic site of FMDV (site A or AnSA) includes a key virus–cell interaction element (RGD motif) located in the Viral Protein 1 (VP1), more precisely at the GH loop. AnSA includes a set of overlapped and mainly linear epitopes, which are the main targets of the humoral immune response. Taking advantage over specific structural features of the GH loop, we have evaluated the influence of every amino acid residue at AnSA in the interaction with 2 neutralizing antibodies by molecular modeling techniques. Additionally, we constructed diverse interaction complexes with multiple site A mutants and discussed about the structural influence of amino acidic insertions in such relevant antigenic site of FMDV. Our approach is in agreement with previous ELISA experiments and allows the understanding of how FMDV mutations may alter the interaction with different antibodies, as we can estimate the contribution of each amino acid to the interaction. Overall, our work contributes to the development of specific vaccination strategies for FMD control. Instituto de Biotecnología Fil: Marrero Diaz De Villegas, Rubén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rodríguez Limardo, Ramiro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Bioinformática Estructural; Argentina Fil: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Turjanski, Adrian G. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina 2019-06-12T12:58:07Z 2019-06-12T12:58:07Z 2015-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0022175915300090 http://hdl.handle.net/20.500.12123/5301 0022-1759 https://doi.org/10.1016/j.jim.2015.06.008 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Immunological Methods 425 : 51-57 (October 2015) |
| spellingShingle | Fiebre Aftosa Enfermedades de los Animales Virus Fiebre Aftosa Anticuerpos Monoclonales Bioinformática Foot and Mouth Disease Animal Diseases Aphthovirus Monoclonal Antibodies Bioinformatics Marrero Diaz De Villegas, Rubén Rodríguez Limardo, Ramiro Carrillo, Elisa Cristina Konig, Guido Alberto Turjanski, Adrián G. A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies |
| title | A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies |
| title_full | A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies |
| title_fullStr | A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies |
| title_full_unstemmed | A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies |
| title_short | A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies |
| title_sort | computational study of the interaction of the foot and mouth disease virus vp1 with monoclonal antibodies |
| topic | Fiebre Aftosa Enfermedades de los Animales Virus Fiebre Aftosa Anticuerpos Monoclonales Bioinformática Foot and Mouth Disease Animal Diseases Aphthovirus Monoclonal Antibodies Bioinformatics |
| url | https://www.sciencedirect.com/science/article/pii/S0022175915300090 http://hdl.handle.net/20.500.12123/5301 https://doi.org/10.1016/j.jim.2015.06.008 |
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