Cytogenetics characterization of Minthostachys mollis (Kunth.) Griseb. Populations from Tucumán province (Argentina)
Minthostachys mollis (Kunth.) Griseb. (Lamiaceae) is a native species from the central and northwest Argentinean region. Reported a chromosome number are 2n = 24 and 2n = 42. In this work, two populations of M. mollis were cytogenetically characterized in Tucumán province, Argentina, by means of mit...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/5299 |
| _version_ | 1855035473785782272 |
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| author | Pastoriza, Adriana del Valle Budeguer, Carlos Jorge Elechosa, Miguel Angel Juarez, Miguel Angel Molina, Ana Maria |
| author_browse | Budeguer, Carlos Jorge Elechosa, Miguel Angel Juarez, Miguel Angel Molina, Ana Maria Pastoriza, Adriana del Valle |
| author_facet | Pastoriza, Adriana del Valle Budeguer, Carlos Jorge Elechosa, Miguel Angel Juarez, Miguel Angel Molina, Ana Maria |
| author_sort | Pastoriza, Adriana del Valle |
| collection | INTA Digital |
| description | Minthostachys mollis (Kunth.) Griseb. (Lamiaceae) is a native species from the central and northwest Argentinean region. Reported a chromosome number are 2n = 24 and 2n = 42. In this work, two populations of M. mollis were cytogenetically characterized in Tucumán province, Argentina, by means of mitosis and meiosis analysis, with material from Río Nío and Villa Padre Monti (Burruyacú, Tucumán). For the mitosis and meiosis analysis, the preparations were made with 2 % haematoxylin as colorant. The pollen grain viability was assessed by using cotton blue in lactophenol technique. Results showed a chromosome number 2n = 48 for both studied populations. In meiosis analysis, normal as well as abnormal divisions in different percentage were observed. Among the abnormalities, multivalent, dyads, triads and polyads of different degree and size were observed. Also, configurations like abnormal spindle orientation, laggard chromosomes and micronucleus in variable percentage were observed. The pollen viability analysis showed 87.2 % of viable pollen grains. The new chromosomal number found in M. mollis could be owing to: a) Formation of gametes (n = 24) from population of Villa Padre Monti and Rio Nio. b) In sympatric zones, with species 2n = 24 and 2n =42, could take place the union of non-reduced gametes (4x = 24), and gametes 4x from those of heptaployds 2n = 42, resulting the populations with 2n = 48. These results constitute a basis in order to perform further studies about the reproductive biology of this species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5299 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán |
| publisherStr | Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán |
| record_format | dspace |
| spelling | INTA52992021-05-31T18:44:11Z Cytogenetics characterization of Minthostachys mollis (Kunth.) Griseb. Populations from Tucumán province (Argentina) Pastoriza, Adriana del Valle Budeguer, Carlos Jorge Elechosa, Miguel Angel Juarez, Miguel Angel Molina, Ana Maria Minthostachys Plantas Aromáticas Plantas Medicinales Citogenética Variación Genética Meiosis Essential Oil Crops Medicinal Plants Cytogenetics Genetic Variation Minthostachys mollis Tucumán, Argentina Minthostachys mollis (Kunth.) Griseb. (Lamiaceae) is a native species from the central and northwest Argentinean region. Reported a chromosome number are 2n = 24 and 2n = 42. In this work, two populations of M. mollis were cytogenetically characterized in Tucumán province, Argentina, by means of mitosis and meiosis analysis, with material from Río Nío and Villa Padre Monti (Burruyacú, Tucumán). For the mitosis and meiosis analysis, the preparations were made with 2 % haematoxylin as colorant. The pollen grain viability was assessed by using cotton blue in lactophenol technique. Results showed a chromosome number 2n = 48 for both studied populations. In meiosis analysis, normal as well as abnormal divisions in different percentage were observed. Among the abnormalities, multivalent, dyads, triads and polyads of different degree and size were observed. Also, configurations like abnormal spindle orientation, laggard chromosomes and micronucleus in variable percentage were observed. The pollen viability analysis showed 87.2 % of viable pollen grains. The new chromosomal number found in M. mollis could be owing to: a) Formation of gametes (n = 24) from population of Villa Padre Monti and Rio Nio. b) In sympatric zones, with species 2n = 24 and 2n =42, could take place the union of non-reduced gametes (4x = 24), and gametes 4x from those of heptaployds 2n = 42, resulting the populations with 2n = 48. These results constitute a basis in order to perform further studies about the reproductive biology of this species. Minthostachys mollis (Kunth.) Griseb. (Lamiaceae) es una especie nativa de la región central y noroeste de Argentina. Se informaron para la especie números cromosómicos 2n = 24 y 2n = 42. Se caracterizaron citogenéticamente dos poblaciones de M. mollis de la provincia de Tucumán, Argentine, mediante el análisis de mitosis y meiosis, con material proveniente de Río Nío y de Villa Padre Monti (Dpto. Burruyacú, Tucumán). Para mitosis y meiosis, se realizaron preparados con hematoxilina al 2 %. La viabilidad de grano de polen se determinó con azul de algodón en lactofenol. Los resultados mostraron un número cromosómico 2n = 48 para las dos poblaciones. En meiosis, se observaron divisiones normales y anormales, en diferente porcentaje. Se presentaron multivalentes, díadas, tríadas y políadas de diferente grado y tamaño. También se observaron configuraciones tales como orientación anormal del huso, rezagados y micronúcleos en porcentaje variable. El estudio de la viabilidad de polen dio un 87,2 % de granos de polen viable. El nuevo número cromosómico encontrado en M. mollis puede deberse a: a) formación de gametas de poblaciones de n = 24 de Villa Padre Monti y Rio Nio. b) En zonas simpátricas, con especies 2n = 24 y 2n = 42, podría tener lugar la unión de gametas no reducidas (4x = 24) y gametas 4x de heptaploides 2n = 42, resultando poblaciones con 2n = 48. Estos resultados constituyen una base para realizar estudios más profundos de la biología reproductiva de esta especie. Instituto de Recursos Biológicos Fil: Pastoriza, Adriana del Valle. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Budeguer, Carlos Jorge. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Elechosa, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Juarez, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Molina, Ana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina 2019-06-12T11:31:07Z 2019-06-12T11:31:07Z 2013-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5299 0080-2069 2314-369X eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán Revista agronómica del noroeste argentino 33 (1) : 15-19 (jul. 2013) |
| spellingShingle | Minthostachys Plantas Aromáticas Plantas Medicinales Citogenética Variación Genética Meiosis Essential Oil Crops Medicinal Plants Cytogenetics Genetic Variation Minthostachys mollis Tucumán, Argentina Pastoriza, Adriana del Valle Budeguer, Carlos Jorge Elechosa, Miguel Angel Juarez, Miguel Angel Molina, Ana Maria Cytogenetics characterization of Minthostachys mollis (Kunth.) Griseb. Populations from Tucumán province (Argentina) |
| title | Cytogenetics characterization of Minthostachys mollis (Kunth.) Griseb. Populations from Tucumán province (Argentina) |
| title_full | Cytogenetics characterization of Minthostachys mollis (Kunth.) Griseb. Populations from Tucumán province (Argentina) |
| title_fullStr | Cytogenetics characterization of Minthostachys mollis (Kunth.) Griseb. Populations from Tucumán province (Argentina) |
| title_full_unstemmed | Cytogenetics characterization of Minthostachys mollis (Kunth.) Griseb. Populations from Tucumán province (Argentina) |
| title_short | Cytogenetics characterization of Minthostachys mollis (Kunth.) Griseb. Populations from Tucumán province (Argentina) |
| title_sort | cytogenetics characterization of minthostachys mollis kunth griseb populations from tucuman province argentina |
| topic | Minthostachys Plantas Aromáticas Plantas Medicinales Citogenética Variación Genética Meiosis Essential Oil Crops Medicinal Plants Cytogenetics Genetic Variation Minthostachys mollis Tucumán, Argentina |
| url | http://hdl.handle.net/20.500.12123/5299 |
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