Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count
Sheep chromosome 3 (Oar3) has the largest number of QTLs reported to be significantly associated with resistance to gastro-intestinal nematodes. This study aimed to identify single nucleotide polymorphisms (SNPs) within candidate genes located in sheep chromosome 3 as well as genes involved in major...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Plos One
2019
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| Subjects: | |
| Online Access: | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088337 http://hdl.handle.net/20.500.12123/5295 https://doi.org/10.1371/journal.pone.0088337 |
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| author | Periasamy, Kathiravan Pichler, Rudolf Poli, Mario Andres Cristel, Silvina Lujan Cetra, Bibiana Maria Medus, Pablo Daniel Basar, Muladno Thiruvenkadan, A.K. Ramasamy, Saravanan Ellahi, Masroor Babbar Mohammed, Faruque Teneva, Atanaska Shamsuddin, Mohammed Garcia Podesta, Mario Diallo, Adama |
| author_browse | Basar, Muladno Cetra, Bibiana Maria Cristel, Silvina Lujan Diallo, Adama Ellahi, Masroor Babbar Garcia Podesta, Mario Medus, Pablo Daniel Mohammed, Faruque Periasamy, Kathiravan Pichler, Rudolf Poli, Mario Andres Ramasamy, Saravanan Shamsuddin, Mohammed Teneva, Atanaska Thiruvenkadan, A.K. |
| author_facet | Periasamy, Kathiravan Pichler, Rudolf Poli, Mario Andres Cristel, Silvina Lujan Cetra, Bibiana Maria Medus, Pablo Daniel Basar, Muladno Thiruvenkadan, A.K. Ramasamy, Saravanan Ellahi, Masroor Babbar Mohammed, Faruque Teneva, Atanaska Shamsuddin, Mohammed Garcia Podesta, Mario Diallo, Adama |
| author_sort | Periasamy, Kathiravan |
| collection | INTA Digital |
| description | Sheep chromosome 3 (Oar3) has the largest number of QTLs reported to be significantly associated with resistance to gastro-intestinal nematodes. This study aimed to identify single nucleotide polymorphisms (SNPs) within candidate genes located in sheep chromosome 3 as well as genes involved in major immune pathways. A total of 41 SNPs were identified across 38 candidate genes in a panel of unrelated sheep and genotyped in 713 animals belonging to 22 breeds across Asia, Europe and South America. The variations and evolution of immune pathway genes were assessed in sheep populations across these macro-environmental regions that significantly differ in the diversity and load of pathogens. The mean minor allele frequency (MAF) did not vary between Asian and European sheep reflecting the absence of ascertainment bias. Phylogenetic analysis revealed two major clusters with most of South Asian, South East Asian and South West Asian breeds clustering together while European and South American sheep breeds clustered together distinctly. Analysis of molecular variance revealed strong phylogeographic structure at loci located in immune pathway genes, unlike microsatellite and genome wide SNP markers. To understand the influence of natural selection processes, SNP loci located in chromosome 3 were utilized to reconstruct haplotypes, the diversity of which showed significant deviations from selective neutrality. Reduced Median network of reconstructed haplotypes showed balancing selection in force at these loci. Preliminary association of SNP genotypes with phenotypes recorded 42 days post challenge revealed significant differences (P<0.05) in fecal egg count, body weight change and packed cell volume at two, four and six SNP loci respectively. In conclusion, the present study reports strong phylogeographic structure and balancing selection operating at SNP loci located within immune pathway genes. Further, SNP loci identified in the study were found to have potential for future large scale association studies in naturally exposed sheep populations. |
| format | Artículo |
| id | INTA5295 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Plos One |
| publisherStr | Plos One |
| record_format | dspace |
| spelling | INTA52952019-06-11T13:18:38Z Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count Periasamy, Kathiravan Pichler, Rudolf Poli, Mario Andres Cristel, Silvina Lujan Cetra, Bibiana Maria Medus, Pablo Daniel Basar, Muladno Thiruvenkadan, A.K. Ramasamy, Saravanan Ellahi, Masroor Babbar Mohammed, Faruque Teneva, Atanaska Shamsuddin, Mohammed Garcia Podesta, Mario Diallo, Adama Ovinos Parásitos Genética Resistencia a las Plagas Respuesta Inmunológica Loci de Rasgos Cuantitativos Sheep Parasites Genetics Pest Resistance Immune Response Quantitative Trait Loci cientifico QTL Sheep chromosome 3 (Oar3) has the largest number of QTLs reported to be significantly associated with resistance to gastro-intestinal nematodes. This study aimed to identify single nucleotide polymorphisms (SNPs) within candidate genes located in sheep chromosome 3 as well as genes involved in major immune pathways. A total of 41 SNPs were identified across 38 candidate genes in a panel of unrelated sheep and genotyped in 713 animals belonging to 22 breeds across Asia, Europe and South America. The variations and evolution of immune pathway genes were assessed in sheep populations across these macro-environmental regions that significantly differ in the diversity and load of pathogens. The mean minor allele frequency (MAF) did not vary between Asian and European sheep reflecting the absence of ascertainment bias. Phylogenetic analysis revealed two major clusters with most of South Asian, South East Asian and South West Asian breeds clustering together while European and South American sheep breeds clustered together distinctly. Analysis of molecular variance revealed strong phylogeographic structure at loci located in immune pathway genes, unlike microsatellite and genome wide SNP markers. To understand the influence of natural selection processes, SNP loci located in chromosome 3 were utilized to reconstruct haplotypes, the diversity of which showed significant deviations from selective neutrality. Reduced Median network of reconstructed haplotypes showed balancing selection in force at these loci. Preliminary association of SNP genotypes with phenotypes recorded 42 days post challenge revealed significant differences (P<0.05) in fecal egg count, body weight change and packed cell volume at two, four and six SNP loci respectively. In conclusion, the present study reports strong phylogeographic structure and balancing selection operating at SNP loci located within immune pathway genes. Further, SNP loci identified in the study were found to have potential for future large scale association studies in naturally exposed sheep populations. Instituto de Genética Fil: Periasamy, Kathiravan. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria Fil: Pichler, Rudolf. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Cristel, Silvina Lujan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Cetra, Bibiana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Medus, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Basar, Muladno. Bogor Agricultural University. Department of Animal Sciences; Indonesia Fil: Thiruvenkadan, A.K. Tamil Nadu Veterinary and Animal Sciences University. Veterinary College and Research Institute-Namakkal; India Fil: Ramasamy, Saravanan. Tamil Nadu Veterinary and Animal Sciences University. Veterinary College and Research Institute-Namakkal; India Fil: Ellahi, Masroor Babbar. University of Veterinary and Animal Sciences. Department of Animal Genetics and Breeding; Paquistán Fil: Mohammed, Faruque. Bangladesh Agricultural University. Department of Animal Breeding and Genetics; Bangladesh Fil: Teneva, Atanaska. University of Forestry; Bulgaria Fil: Shamsuddin, Mohammed. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria Fil: Garcia Podesta, Mario. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria Fil: Diallo, Adama. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria 2019-06-11T13:17:16Z 2019-06-11T13:17:16Z 2014-02-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088337 http://hdl.handle.net/20.500.12123/5295 1932-6203 https://doi.org/10.1371/journal.pone.0088337 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plos One PLoS ONE 9 (2) : e88337. (February 2014) |
| spellingShingle | Ovinos Parásitos Genética Resistencia a las Plagas Respuesta Inmunológica Loci de Rasgos Cuantitativos Sheep Parasites Genetics Pest Resistance Immune Response Quantitative Trait Loci cientifico QTL Periasamy, Kathiravan Pichler, Rudolf Poli, Mario Andres Cristel, Silvina Lujan Cetra, Bibiana Maria Medus, Pablo Daniel Basar, Muladno Thiruvenkadan, A.K. Ramasamy, Saravanan Ellahi, Masroor Babbar Mohammed, Faruque Teneva, Atanaska Shamsuddin, Mohammed Garcia Podesta, Mario Diallo, Adama Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count |
| title | Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count |
| title_full | Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count |
| title_fullStr | Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count |
| title_full_unstemmed | Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count |
| title_short | Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count |
| title_sort | candidate gene approach for parasite resistance in sheep variation in immune pathway genes and association with fecal egg count |
| topic | Ovinos Parásitos Genética Resistencia a las Plagas Respuesta Inmunológica Loci de Rasgos Cuantitativos Sheep Parasites Genetics Pest Resistance Immune Response Quantitative Trait Loci cientifico QTL |
| url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088337 http://hdl.handle.net/20.500.12123/5295 https://doi.org/10.1371/journal.pone.0088337 |
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