Vitrified embryo transfer in Merino sheep under extensive conditions
The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate the influence of embryo stage in such pregnancy rate. One hundred and twenty-seven embryos were rewarmed and transferred i...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Brazilian College of Animal Reproduction
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/5229 http://www.animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0108 http://dx.doi.org/10.21451/1984-3143-AR2018-0108 |
| _version_ | 1855035461258444800 |
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| author | Gibbons, Alejandro Eduardo Bruno Galarraga, Maria Macarena Fernandez, Jimena Gonzalez Bulnes, Antonio Cueto, Marcela Isabel |
| author_browse | Bruno Galarraga, Maria Macarena Cueto, Marcela Isabel Fernandez, Jimena Gibbons, Alejandro Eduardo Gonzalez Bulnes, Antonio |
| author_facet | Gibbons, Alejandro Eduardo Bruno Galarraga, Maria Macarena Fernandez, Jimena Gonzalez Bulnes, Antonio Cueto, Marcela Isabel |
| author_sort | Gibbons, Alejandro Eduardo |
| collection | INTA Digital |
| description | The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate the influence of embryo stage in such pregnancy rate. One hundred and twenty-seven embryos were rewarmed and transferred into recipient ewes. On rewarming, the embryos were placed into three-step cryoprotectant dilutions. Finally, prior to transfer to recipient females, embryos were maintained in Basic Medium for 5 min at 25ºC and were re-evaluated by morphological criteria; all degenerated embryos were eliminated. Recipient ewes (n = 150) were treated for estrus with sponges placed for 14 days and 300 IU of eCG. At embryo transfer, three experimental groups were defined: morulae transferred on Day 7, blastocysts transferred on Day 7 and blastocysts transferred on Day 8 after sponge removal. In all groups, semi-laparoscopic transfer of one rewarmed embryo per recipient was performed. Pregnancy was diagnosed by ultrasonography on day 28 after embryo transfer. The embryo selection rate after rewarming was higher for blastocysts (89.3% - 67/75) compared to morulae (65.9% - 60/91) (P < 0.05). Pregnancy diagnosis showed a 38.3% (23/60) of success after morula transfer on Day 7 post progestagen removal. The day of transfer showed a significant influence on pregnancy rate after blastocyst transfer (Day 8, 55.9% - 19/34 vs Day 7, 21.2% - 7/33) (P < 0.05). Blastocysts transfer on Day 8 showed the highest global efficiency (pregnancies/total embryos after rewarming) (47.5% - 19/40) (P < 0.05). In conclusion, reproductive efficiency obtained by vitrified embryo transfer allows its recommendation for embryo transfer programs under extensive conditions. The importance of considering the synchrony between the embryo age and the recipient uterus stage is emphasized. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5229 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Brazilian College of Animal Reproduction |
| publisherStr | Brazilian College of Animal Reproduction |
| record_format | dspace |
| spelling | INTA52292019-06-03T12:49:03Z Vitrified embryo transfer in Merino sheep under extensive conditions Gibbons, Alejandro Eduardo Bruno Galarraga, Maria Macarena Fernandez, Jimena Gonzalez Bulnes, Antonio Cueto, Marcela Isabel Inseminación Artificial Inseminación in Vitro Reproducción Dirigida Transferencia de Embriones Artificial Insemination In Vitro Insemination Reproduction Control Embryo Transfer Sheep Ovinos Raza Merino The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate the influence of embryo stage in such pregnancy rate. One hundred and twenty-seven embryos were rewarmed and transferred into recipient ewes. On rewarming, the embryos were placed into three-step cryoprotectant dilutions. Finally, prior to transfer to recipient females, embryos were maintained in Basic Medium for 5 min at 25ºC and were re-evaluated by morphological criteria; all degenerated embryos were eliminated. Recipient ewes (n = 150) were treated for estrus with sponges placed for 14 days and 300 IU of eCG. At embryo transfer, three experimental groups were defined: morulae transferred on Day 7, blastocysts transferred on Day 7 and blastocysts transferred on Day 8 after sponge removal. In all groups, semi-laparoscopic transfer of one rewarmed embryo per recipient was performed. Pregnancy was diagnosed by ultrasonography on day 28 after embryo transfer. The embryo selection rate after rewarming was higher for blastocysts (89.3% - 67/75) compared to morulae (65.9% - 60/91) (P < 0.05). Pregnancy diagnosis showed a 38.3% (23/60) of success after morula transfer on Day 7 post progestagen removal. The day of transfer showed a significant influence on pregnancy rate after blastocyst transfer (Day 8, 55.9% - 19/34 vs Day 7, 21.2% - 7/33) (P < 0.05). Blastocysts transfer on Day 8 showed the highest global efficiency (pregnancies/total embryos after rewarming) (47.5% - 19/40) (P < 0.05). In conclusion, reproductive efficiency obtained by vitrified embryo transfer allows its recommendation for embryo transfer programs under extensive conditions. The importance of considering the synchrony between the embryo age and the recipient uterus stage is emphasized. Estación Experimental Agropecuaria Bariloche Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Laboratorio de Reproducción de Rumiantes Menores; Argentina Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina Fil: Fernández, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina Fil: Gonzalez Bulnes, Antonio. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Reproducción Animal; España Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina 2019-06-03T12:34:40Z 2019-06-03T12:34:40Z 2019-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5229 http://www.animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0108 1984-3143 http://dx.doi.org/10.21451/1984-3143-AR2018-0108 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Brazilian College of Animal Reproduction Animal Reproduction 16 (2) : 297-301 (Junio 2019) |
| spellingShingle | Inseminación Artificial Inseminación in Vitro Reproducción Dirigida Transferencia de Embriones Artificial Insemination In Vitro Insemination Reproduction Control Embryo Transfer Sheep Ovinos Raza Merino Gibbons, Alejandro Eduardo Bruno Galarraga, Maria Macarena Fernandez, Jimena Gonzalez Bulnes, Antonio Cueto, Marcela Isabel Vitrified embryo transfer in Merino sheep under extensive conditions |
| title | Vitrified embryo transfer in Merino sheep under extensive conditions |
| title_full | Vitrified embryo transfer in Merino sheep under extensive conditions |
| title_fullStr | Vitrified embryo transfer in Merino sheep under extensive conditions |
| title_full_unstemmed | Vitrified embryo transfer in Merino sheep under extensive conditions |
| title_short | Vitrified embryo transfer in Merino sheep under extensive conditions |
| title_sort | vitrified embryo transfer in merino sheep under extensive conditions |
| topic | Inseminación Artificial Inseminación in Vitro Reproducción Dirigida Transferencia de Embriones Artificial Insemination In Vitro Insemination Reproduction Control Embryo Transfer Sheep Ovinos Raza Merino |
| url | http://hdl.handle.net/20.500.12123/5229 http://www.animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0108 http://dx.doi.org/10.21451/1984-3143-AR2018-0108 |
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