Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit
Fleshy fruit are susceptible to develop chilling injury (CI) disorders when stored at low temperatures for extended periods during the postharvest. In peach (Prunus persica L. Batsch) fruit, the woolly texture, expressed as a lack of juiciness, is the most important CI symptom. In the present work,...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
|
| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0925521418310895 http://hdl.handle.net/20.500.12123/5211 https://doi.org/10.1016/j.postharvbio.2019.02.007 |
| _version_ | 1855483446220029952 |
|---|---|
| author | Monti, Laura Lucia Bustamante, Claudia Anabel Budde, Claudio Olaf Gabilondo, Julieta Müller, Gabriela Leticia Lara, Maria Valeria Drincovich, María Fabiana |
| author_browse | Budde, Claudio Olaf Bustamante, Claudia Anabel Drincovich, María Fabiana Gabilondo, Julieta Lara, Maria Valeria Monti, Laura Lucia Müller, Gabriela Leticia |
| author_facet | Monti, Laura Lucia Bustamante, Claudia Anabel Budde, Claudio Olaf Gabilondo, Julieta Müller, Gabriela Leticia Lara, Maria Valeria Drincovich, María Fabiana |
| author_sort | Monti, Laura Lucia |
| collection | INTA Digital |
| description | Fleshy fruit are susceptible to develop chilling injury (CI) disorders when stored at low temperatures for extended periods during the postharvest. In peach (Prunus persica L. Batsch) fruit, the woolly texture, expressed as a lack of juiciness, is the most important CI symptom. In the present work, we set up to study the metabolomic and proteomic differences of Spring Lady peach fruit, which, despite had been subjected to the same postharvest treatment (24 d at 0 °C followed by 5 d at 20 °C), display a contrasting woolliness phenotype. A drastic decrease in the content of sugars and sugar alcohols (sucrose, fructose, glucose, ribose, xylose, galactose, sorbitol and raffinose), as well as in all the amino acids detected (Ala, Asn, Gly, Glu, Ile, Ser, Thr, and Val), was found in woolly (WF) when compared to juicy fruit (JF). The quantitative proteomic analysis of JF and WF identified 227 differentially expressed proteins (DEP), from which 165 were increased and 62 decreased in WF with respect to JF. The identification of clusters of functionally associated proteins among the DEP revealed biochemical and physiological processes linked to the development of the woolly phenotype; such as sugar catabolism, amino acid usage, and proteome reconfiguration. On the other hand, a decrease of proteins involved in reactive oxygen species (ROS) detoxification, lower ascorbate peroxidase activity, and higher ROS levels, were found in the mesocarp of WF when compared to JF. Overall, this work identifies the induction of carbon oxidation processes, proteome reconfiguration, and increased ROS generation, as relevant metabolic signatures of woolliness development in peach fruit. Besides, the experimental approach used here clearly shows that the woolly phenotype can be triggered in similar biochemical contexts. The relevance of oxidative stress, probably as a consequence of an imbalance between ROS production and elimination, is highlighted as a causal mechanism of woolliness development in peach fruit. |
| format | Artículo |
| id | INTA5211 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA52112019-06-06T17:12:09Z Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit Monti, Laura Lucia Bustamante, Claudia Anabel Budde, Claudio Olaf Gabilondo, Julieta Müller, Gabriela Leticia Lara, Maria Valeria Drincovich, María Fabiana Durazno Almacenamiento en Frío Deterioro Fisiología Postcosecha Peaches Cold Storage Deterioration Postharvest Physiology Metaboloma Proteoma Woolliness Daño por Frío Metabolome Proteome Lanosidad Chilling Injury Fleshy fruit are susceptible to develop chilling injury (CI) disorders when stored at low temperatures for extended periods during the postharvest. In peach (Prunus persica L. Batsch) fruit, the woolly texture, expressed as a lack of juiciness, is the most important CI symptom. In the present work, we set up to study the metabolomic and proteomic differences of Spring Lady peach fruit, which, despite had been subjected to the same postharvest treatment (24 d at 0 °C followed by 5 d at 20 °C), display a contrasting woolliness phenotype. A drastic decrease in the content of sugars and sugar alcohols (sucrose, fructose, glucose, ribose, xylose, galactose, sorbitol and raffinose), as well as in all the amino acids detected (Ala, Asn, Gly, Glu, Ile, Ser, Thr, and Val), was found in woolly (WF) when compared to juicy fruit (JF). The quantitative proteomic analysis of JF and WF identified 227 differentially expressed proteins (DEP), from which 165 were increased and 62 decreased in WF with respect to JF. The identification of clusters of functionally associated proteins among the DEP revealed biochemical and physiological processes linked to the development of the woolly phenotype; such as sugar catabolism, amino acid usage, and proteome reconfiguration. On the other hand, a decrease of proteins involved in reactive oxygen species (ROS) detoxification, lower ascorbate peroxidase activity, and higher ROS levels, were found in the mesocarp of WF when compared to JF. Overall, this work identifies the induction of carbon oxidation processes, proteome reconfiguration, and increased ROS generation, as relevant metabolic signatures of woolliness development in peach fruit. Besides, the experimental approach used here clearly shows that the woolly phenotype can be triggered in similar biochemical contexts. The relevance of oxidative stress, probably as a consequence of an imbalance between ROS production and elimination, is highlighted as a causal mechanism of woolliness development in peach fruit. EEA San Pedro Fil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina 2019-05-28T12:17:01Z 2019-05-28T12:17:01Z 2019 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0925521418310895 http://hdl.handle.net/20.500.12123/5211 0925-5214 https://doi.org/10.1016/j.postharvbio.2019.02.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Postharvest Biology and Technology 151 : 142-151. (May 2019) |
| spellingShingle | Durazno Almacenamiento en Frío Deterioro Fisiología Postcosecha Peaches Cold Storage Deterioration Postharvest Physiology Metaboloma Proteoma Woolliness Daño por Frío Metabolome Proteome Lanosidad Chilling Injury Monti, Laura Lucia Bustamante, Claudia Anabel Budde, Claudio Olaf Gabilondo, Julieta Müller, Gabriela Leticia Lara, Maria Valeria Drincovich, María Fabiana Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit |
| title | Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit |
| title_full | Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit |
| title_fullStr | Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit |
| title_full_unstemmed | Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit |
| title_short | Metabolomic and proteomic profiling of Spring Lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling-injured fruit |
| title_sort | metabolomic and proteomic profiling of spring lady peach fruit with contrasting woolliness phenotype reveals carbon oxidative processes and proteome reconfiguration in chilling injured fruit |
| topic | Durazno Almacenamiento en Frío Deterioro Fisiología Postcosecha Peaches Cold Storage Deterioration Postharvest Physiology Metaboloma Proteoma Woolliness Daño por Frío Metabolome Proteome Lanosidad Chilling Injury |
| url | https://www.sciencedirect.com/science/article/pii/S0925521418310895 http://hdl.handle.net/20.500.12123/5211 https://doi.org/10.1016/j.postharvbio.2019.02.007 |
| work_keys_str_mv | AT montilauralucia metabolomicandproteomicprofilingofspringladypeachfruitwithcontrastingwoollinessphenotyperevealscarbonoxidativeprocessesandproteomereconfigurationinchillinginjuredfruit AT bustamanteclaudiaanabel metabolomicandproteomicprofilingofspringladypeachfruitwithcontrastingwoollinessphenotyperevealscarbonoxidativeprocessesandproteomereconfigurationinchillinginjuredfruit AT buddeclaudioolaf metabolomicandproteomicprofilingofspringladypeachfruitwithcontrastingwoollinessphenotyperevealscarbonoxidativeprocessesandproteomereconfigurationinchillinginjuredfruit AT gabilondojulieta metabolomicandproteomicprofilingofspringladypeachfruitwithcontrastingwoollinessphenotyperevealscarbonoxidativeprocessesandproteomereconfigurationinchillinginjuredfruit AT mullergabrielaleticia metabolomicandproteomicprofilingofspringladypeachfruitwithcontrastingwoollinessphenotyperevealscarbonoxidativeprocessesandproteomereconfigurationinchillinginjuredfruit AT laramariavaleria metabolomicandproteomicprofilingofspringladypeachfruitwithcontrastingwoollinessphenotyperevealscarbonoxidativeprocessesandproteomereconfigurationinchillinginjuredfruit AT drincovichmariafabiana metabolomicandproteomicprofilingofspringladypeachfruitwithcontrastingwoollinessphenotyperevealscarbonoxidativeprocessesandproteomereconfigurationinchillinginjuredfruit |