Genetic structure in cultivated quinoa (Chenopodium quinoa Willd.), a reflection of landscape structure in Northwest Argentina

Quinoa (Chenopodium quinoa Willd.), one of the main crops domesticated in the Andean highlands 1,000 of years ago, played an important role as a protein source. 35 germplasm accessions collected along the Northwest Argentina (NWA) region were studied using 22 microsatellite (SSR) markers. Results sh...

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Main Authors: Costa Tártara, Sabrina María, Manifesto, Maria Marcela, Bertero, Héctor Daniel, Bramardi, Sergio Jorge
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Springer 2019
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/5157
https://link.springer.com/article/10.1007/s10592-012-0350-1
https://doi.org/10.1007/s10592-012-0350-1
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author Costa Tártara, Sabrina María
Manifesto, Maria Marcela
Bertero, Héctor Daniel
Bramardi, Sergio Jorge
author_browse Bertero, Héctor Daniel
Bramardi, Sergio Jorge
Costa Tártara, Sabrina María
Manifesto, Maria Marcela
author_facet Costa Tártara, Sabrina María
Manifesto, Maria Marcela
Bertero, Héctor Daniel
Bramardi, Sergio Jorge
author_sort Costa Tártara, Sabrina María
collection INTA Digital
description Quinoa (Chenopodium quinoa Willd.), one of the main crops domesticated in the Andean highlands 1,000 of years ago, played an important role as a protein source. 35 germplasm accessions collected along the Northwest Argentina (NWA) region were studied using 22 microsatellite (SSR) markers. Results showed a great level of genetic diversity, differing from previous reports about the geographical distribution of quinoa variability. All SSR loci analysed were highly polymorphic detecting a total of 354 alleles among all populations, with an average of 16 alleles per locus. Cluster analyses grouped the accessions into four main clusters at the average genetic distance level (0.80), each of which represented a different environment of the NWA region: Puna (UHe = 0.42, ±0.07 SE), Dry Valleys (UHe = 0.27, ±0.05 SE), Eastern Humid Valleys (UHe = 0.16, ±0.04 SE) and a transition area with high altitudes between the last two environments (UHe = 0.25, ±0.03 SE). An eastward decreasing genetic diversity gradient was found. AMOVA analyses showed a strong genetic structure: a high population subdivision relative to the grouping by region (Fsr = 0.47) together with a high genetic differentiation among populations (Fst = 0.58) and a heterozygous defect (Fis = 0.63) in each of them. The variability structure, a reflection of the structure of the NWA landscapes, is discussed in connection with environmental variables.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA51572019-06-07T17:59:17Z Genetic structure in cultivated quinoa (Chenopodium quinoa Willd.), a reflection of landscape structure in Northwest Argentina Costa Tártara, Sabrina María Manifesto, Maria Marcela Bertero, Héctor Daniel Bramardi, Sergio Jorge Chenopodium Quinoa Variación Genética Germoplasma Microsatélites Muestreo Cluster Genetic Structures Genetic Variation Germplasm Microsatellites Cluster Sampling Estructura Genética Quinoa Argentina Región Noroeste Cluster Analysis Análisis de Cluster Quinoa (Chenopodium quinoa Willd.), one of the main crops domesticated in the Andean highlands 1,000 of years ago, played an important role as a protein source. 35 germplasm accessions collected along the Northwest Argentina (NWA) region were studied using 22 microsatellite (SSR) markers. Results showed a great level of genetic diversity, differing from previous reports about the geographical distribution of quinoa variability. All SSR loci analysed were highly polymorphic detecting a total of 354 alleles among all populations, with an average of 16 alleles per locus. Cluster analyses grouped the accessions into four main clusters at the average genetic distance level (0.80), each of which represented a different environment of the NWA region: Puna (UHe = 0.42, ±0.07 SE), Dry Valleys (UHe = 0.27, ±0.05 SE), Eastern Humid Valleys (UHe = 0.16, ±0.04 SE) and a transition area with high altitudes between the last two environments (UHe = 0.25, ±0.03 SE). An eastward decreasing genetic diversity gradient was found. AMOVA analyses showed a strong genetic structure: a high population subdivision relative to the grouping by region (Fsr = 0.47) together with a high genetic differentiation among populations (Fst = 0.58) and a heterozygous defect (Fis = 0.63) in each of them. The variability structure, a reflection of the structure of the NWA landscapes, is discussed in connection with environmental variables. Instituto de Recursos Biológicos Fil: Costa Tártara, Sabrina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Bertero, Hector Daniel. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bramardi, Sergio J. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina 2019-05-20T14:04:55Z 2019-05-20T14:04:55Z 2012-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/5157 https://link.springer.com/article/10.1007/s10592-012-0350-1 1566-0621 1572-9737 (Online) https://doi.org/10.1007/s10592-012-0350-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Springer Conservation genetics 13 (4) : 1027–1038. (August 2012)
spellingShingle Chenopodium Quinoa
Variación Genética
Germoplasma
Microsatélites
Muestreo Cluster
Genetic Structures
Genetic Variation
Germplasm
Microsatellites
Cluster Sampling
Estructura Genética
Quinoa
Argentina
Región Noroeste
Cluster Analysis
Análisis de Cluster
Costa Tártara, Sabrina María
Manifesto, Maria Marcela
Bertero, Héctor Daniel
Bramardi, Sergio Jorge
Genetic structure in cultivated quinoa (Chenopodium quinoa Willd.), a reflection of landscape structure in Northwest Argentina
title Genetic structure in cultivated quinoa (Chenopodium quinoa Willd.), a reflection of landscape structure in Northwest Argentina
title_full Genetic structure in cultivated quinoa (Chenopodium quinoa Willd.), a reflection of landscape structure in Northwest Argentina
title_fullStr Genetic structure in cultivated quinoa (Chenopodium quinoa Willd.), a reflection of landscape structure in Northwest Argentina
title_full_unstemmed Genetic structure in cultivated quinoa (Chenopodium quinoa Willd.), a reflection of landscape structure in Northwest Argentina
title_short Genetic structure in cultivated quinoa (Chenopodium quinoa Willd.), a reflection of landscape structure in Northwest Argentina
title_sort genetic structure in cultivated quinoa chenopodium quinoa willd a reflection of landscape structure in northwest argentina
topic Chenopodium Quinoa
Variación Genética
Germoplasma
Microsatélites
Muestreo Cluster
Genetic Structures
Genetic Variation
Germplasm
Microsatellites
Cluster Sampling
Estructura Genética
Quinoa
Argentina
Región Noroeste
Cluster Analysis
Análisis de Cluster
url http://hdl.handle.net/20.500.12123/5157
https://link.springer.com/article/10.1007/s10592-012-0350-1
https://doi.org/10.1007/s10592-012-0350-1
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