IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characteriz...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0041010119300972 http://hdl.handle.net/20.500.12123/5141 https://doi.org/10.1016/j.toxicon.2019.03.020 |
| _version_ | 1855483563524227072 |
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| author | Leiva, Carlos Leónidas Cangelosi, Adriana Mariconda, Virginia Farace, Mariano Geoghegan, Patricia Brero, Luisa Fernandez Miyakawa, Mariano Enrique Chacana, Pablo Anibal |
| author_browse | Brero, Luisa Cangelosi, Adriana Chacana, Pablo Anibal Farace, Mariano Fernandez Miyakawa, Mariano Enrique Geoghegan, Patricia Leiva, Carlos Leónidas Mariconda, Virginia |
| author_facet | Leiva, Carlos Leónidas Cangelosi, Adriana Mariconda, Virginia Farace, Mariano Geoghegan, Patricia Brero, Luisa Fernandez Miyakawa, Mariano Enrique Chacana, Pablo Anibal |
| author_sort | Leiva, Carlos Leónidas |
| collection | INTA Digital |
| description | Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characterized and assessed the efficacy of IgY-based antivenoms against B. alternatus venom. Immunochemical studies (reactivity, avidity and antigen recognition pattern) as well as antivenom efficacy assays were performed. After the 3rd immunization, levels of specific IgY reached a maximum that was maintained throughout the observation period, while avidity indexes of the extracts increased after the successive immunizations. Furthermore, IgY against B. alternatus recognized protein complexes of the venom with high (>40 kDa), medium (20–40 kDa) and low (<20 kDa) molecular weights. IgY antivenoms obtained after 8 immunizations neutralized 35.65 μg of B. alternatus venom per mg of antivenom, while specific activities values ranged from 0.28 to 0.42. In conclusion, we produced and characterized IgY antivenoms capable of neutralizing the lethal activity of B. alternatus venom at a preclinical level. Thus, IgY-technology may allow the production of effective and affordable antivenoms fulfilling the urgent needs of many countries where conventional manufacture is unable to provide enough availability of antivenoms. |
| format | Artículo |
| id | INTA5141 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA51412019-06-10T18:16:37Z IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy Leiva, Carlos Leónidas Cangelosi, Adriana Mariconda, Virginia Farace, Mariano Geoghegan, Patricia Brero, Luisa Fernandez Miyakawa, Mariano Enrique Chacana, Pablo Anibal Egg Yolk Snake Bites Venoms Antigens Yema de Huevo Mordeduras de Serpientes Venenos Antígenos Bothrops alternatus Veneno de Serpiente Anticuerpo IgY Neutralización del Veneno Snake Venom IgY Antibody Venom Neutralization Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characterized and assessed the efficacy of IgY-based antivenoms against B. alternatus venom. Immunochemical studies (reactivity, avidity and antigen recognition pattern) as well as antivenom efficacy assays were performed. After the 3rd immunization, levels of specific IgY reached a maximum that was maintained throughout the observation period, while avidity indexes of the extracts increased after the successive immunizations. Furthermore, IgY against B. alternatus recognized protein complexes of the venom with high (>40 kDa), medium (20–40 kDa) and low (<20 kDa) molecular weights. IgY antivenoms obtained after 8 immunizations neutralized 35.65 μg of B. alternatus venom per mg of antivenom, while specific activities values ranged from 0.28 to 0.42. In conclusion, we produced and characterized IgY antivenoms capable of neutralizing the lethal activity of B. alternatus venom at a preclinical level. Thus, IgY-technology may allow the production of effective and affordable antivenoms fulfilling the urgent needs of many countries where conventional manufacture is unable to provide enough availability of antivenoms. Instituto de Patobiología Fil: Leiva, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Farace, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina 2019-05-17T13:07:57Z 2019-05-17T13:07:57Z 2019-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0041010119300972 http://hdl.handle.net/20.500.12123/5141 0041-0101 https://doi.org/10.1016/j.toxicon.2019.03.020 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Toxicon 163 : 84-92 (Mayo 2019) |
| spellingShingle | Egg Yolk Snake Bites Venoms Antigens Yema de Huevo Mordeduras de Serpientes Venenos Antígenos Bothrops alternatus Veneno de Serpiente Anticuerpo IgY Neutralización del Veneno Snake Venom IgY Antibody Venom Neutralization Leiva, Carlos Leónidas Cangelosi, Adriana Mariconda, Virginia Farace, Mariano Geoghegan, Patricia Brero, Luisa Fernandez Miyakawa, Mariano Enrique Chacana, Pablo Anibal IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy |
| title | IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy |
| title_full | IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy |
| title_fullStr | IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy |
| title_full_unstemmed | IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy |
| title_short | IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy |
| title_sort | igy based antivenom against bothrops alternatus production and neutralization efficacy |
| topic | Egg Yolk Snake Bites Venoms Antigens Yema de Huevo Mordeduras de Serpientes Venenos Antígenos Bothrops alternatus Veneno de Serpiente Anticuerpo IgY Neutralización del Veneno Snake Venom IgY Antibody Venom Neutralization |
| url | https://www.sciencedirect.com/science/article/pii/S0041010119300972 http://hdl.handle.net/20.500.12123/5141 https://doi.org/10.1016/j.toxicon.2019.03.020 |
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