The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America
A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised uredini...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2019
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| Online Access: | https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300 http://hdl.handle.net/20.500.12123/5140 https://doi.org/10.1111/ppa.12300 |
| _version_ | 1855035445452210176 |
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| author | Jorge, V.R. Silva, M.R. Guillin, Eduardo Alejandro Freire, M.C.M. Schuster, I. Almeida, A.M.R. Oliveira, Luiz O. |
| author_browse | Almeida, A.M.R. Freire, M.C.M. Guillin, Eduardo Alejandro Jorge, V.R. Oliveira, Luiz O. Schuster, I. Silva, M.R. |
| author_facet | Jorge, V.R. Silva, M.R. Guillin, Eduardo Alejandro Freire, M.C.M. Schuster, I. Almeida, A.M.R. Oliveira, Luiz O. |
| author_sort | Jorge, V.R. |
| collection | INTA Digital |
| description | A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5140 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA51402019-05-17T13:05:52Z The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America Jorge, V.R. Silva, M.R. Guillin, Eduardo Alejandro Freire, M.C.M. Schuster, I. Almeida, A.M.R. Oliveira, Luiz O. Soja Enfermedades de las Plantas Roya Phakopsora pachyrhizi Variación Genética América del Sur Soybeans Plant Diseases Rusts Genetic Variation South America Roya de la Soja Roya Asiática Diversidad Genética A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations. Instituto de Genética Fil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; Brasil Fil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; Brasil Fil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil 2019-05-17T13:03:49Z 2019-05-17T13:03:49Z 2015-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300 http://hdl.handle.net/20.500.12123/5140 0032-0862 1365-3059 https://doi.org/10.1111/ppa.12300 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Plant Pathology 64 (3) : 729-737 (June 2015) |
| spellingShingle | Soja Enfermedades de las Plantas Roya Phakopsora pachyrhizi Variación Genética América del Sur Soybeans Plant Diseases Rusts Genetic Variation South America Roya de la Soja Roya Asiática Diversidad Genética Jorge, V.R. Silva, M.R. Guillin, Eduardo Alejandro Freire, M.C.M. Schuster, I. Almeida, A.M.R. Oliveira, Luiz O. The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
| title | The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
| title_full | The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
| title_fullStr | The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
| title_full_unstemmed | The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
| title_short | The origin and genetic diversity of the causal agent of Asian soybean rust, Phakopsora pachyrhizi, in South America |
| title_sort | origin and genetic diversity of the causal agent of asian soybean rust phakopsora pachyrhizi in south america |
| topic | Soja Enfermedades de las Plantas Roya Phakopsora pachyrhizi Variación Genética América del Sur Soybeans Plant Diseases Rusts Genetic Variation South America Roya de la Soja Roya Asiática Diversidad Genética |
| url | https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12300 http://hdl.handle.net/20.500.12123/5140 https://doi.org/10.1111/ppa.12300 |
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