LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component system (Pst–Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) modulates virulence against A. thaliana. Bioinformatic analysis and the e...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | http://onlinelibrary.wiley.com/doi/10.1111/tpj.12289/abstract http://hdl.handle.net/20.500.12123/5128 https://doi.org/10.1111/tpj.12289 |
| _version_ | 1855483561023373312 |
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| author | Moriconi, Victoria Sellaro, Romina Vanesa Ayub, Nicolás Daniel Soto, Gabriela Rugnone, Matias Leandro Shah, Rashmi Pathak, Gopal P. Gärtner, Wolfgang Casal, Jorge José |
| author_browse | Ayub, Nicolás Daniel Casal, Jorge José Gärtner, Wolfgang Moriconi, Victoria Pathak, Gopal P. Rugnone, Matias Leandro Sellaro, Romina Vanesa Shah, Rashmi Soto, Gabriela |
| author_facet | Moriconi, Victoria Sellaro, Romina Vanesa Ayub, Nicolás Daniel Soto, Gabriela Rugnone, Matias Leandro Shah, Rashmi Pathak, Gopal P. Gärtner, Wolfgang Casal, Jorge José |
| author_sort | Moriconi, Victoria |
| collection | INTA Digital |
| description | In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component system (Pst–Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) modulates virulence against A. thaliana. Bioinformatic analysis and the existence of an episomal circular intermediate indicate that the locus encoding Pst–Lov is present in an active genomic island acquired by horizontal transfer. Strains mutated at Pst–Lov showed enhanced growth on minimal medium and in leaves of A. thaliana exposed to light, but not in leaves incubated in darkness or buried in the soil. Pst–Lov repressed the expression of principal and alternative sigma factor genes and their downstream targets linked to bacterial growth, virulence and quorum sensing, in a strictly light‐dependent manner. We propose that the function of Pst–Lov is to distinguish between soil (dark) and leaf (light) environments, attenuating the damage caused to host tissues while releasing growth out of the host. Therefore, in addition to its direct actions via photosynthesis and plant sensory receptors, light may affect plants indirectly via the sensory receptors of bacterial pathogens. |
| format | Artículo |
| id | INTA5128 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA51282019-05-16T12:52:51Z LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves Moriconi, Victoria Sellaro, Romina Vanesa Ayub, Nicolás Daniel Soto, Gabriela Rugnone, Matias Leandro Shah, Rashmi Pathak, Gopal P. Gärtner, Wolfgang Casal, Jorge José Arabidopsis Thaliana Pseudomonas syringae Tomate Regimenes de Luz Bioinformática Tomatoes Light Regimes Bioinformatics In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component system (Pst–Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) modulates virulence against A. thaliana. Bioinformatic analysis and the existence of an episomal circular intermediate indicate that the locus encoding Pst–Lov is present in an active genomic island acquired by horizontal transfer. Strains mutated at Pst–Lov showed enhanced growth on minimal medium and in leaves of A. thaliana exposed to light, but not in leaves incubated in darkness or buried in the soil. Pst–Lov repressed the expression of principal and alternative sigma factor genes and their downstream targets linked to bacterial growth, virulence and quorum sensing, in a strictly light‐dependent manner. We propose that the function of Pst–Lov is to distinguish between soil (dark) and leaf (light) environments, attenuating the damage caused to host tissues while releasing growth out of the host. Therefore, in addition to its direct actions via photosynthesis and plant sensory receptors, light may affect plants indirectly via the sensory receptors of bacterial pathogens. Instituto de Genética Fil: Moriconi, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina Fil: Sellaro, Romina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Rugnone, Matias Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina Fil: Shah, Rashmi. Max Planck Institute for Chemical Energy Conversion; Alemania Fil: Pathak, Gopal P.. Max Planck Institute for Chemical Energy Conversion; Alemania Fil: Gärtner, Wolfgang. Max Planck Institute for Chemical Energy Conversion; Alemania Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina 2019-05-16T12:51:23Z 2019-05-16T12:51:23Z 2013-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://onlinelibrary.wiley.com/doi/10.1111/tpj.12289/abstract http://hdl.handle.net/20.500.12123/5128 0960-7412 1365-313X https://doi.org/10.1111/tpj.12289 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant Journal 76 (2) : 322-331 (October 2013) |
| spellingShingle | Arabidopsis Thaliana Pseudomonas syringae Tomate Regimenes de Luz Bioinformática Tomatoes Light Regimes Bioinformatics Moriconi, Victoria Sellaro, Romina Vanesa Ayub, Nicolás Daniel Soto, Gabriela Rugnone, Matias Leandro Shah, Rashmi Pathak, Gopal P. Gärtner, Wolfgang Casal, Jorge José LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves |
| title | LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves |
| title_full | LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves |
| title_fullStr | LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves |
| title_full_unstemmed | LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves |
| title_short | LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves |
| title_sort | lov domain photoreceptor encoded in a genomic island attenuates the virulence of pseudomonas syringae in light exposed arabidopsis leaves |
| topic | Arabidopsis Thaliana Pseudomonas syringae Tomate Regimenes de Luz Bioinformática Tomatoes Light Regimes Bioinformatics |
| url | http://onlinelibrary.wiley.com/doi/10.1111/tpj.12289/abstract http://hdl.handle.net/20.500.12123/5128 https://doi.org/10.1111/tpj.12289 |
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