Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellu...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Taylor & Francis
2019
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| Online Access: | https://www.tandfonline.com/doi/full/10.1080/25765299.2019.1598107 http://hdl.handle.net/20.500.12123/5122 https://doi.org/10.1080/25765299.2019.1598107 |
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| author | Coniglio, Romina O. Fonseca, María I. Diaz, Gabriela V. Ontañon, Ornella Mailén Ghio, Silvina Campos, Eleonora Zapata, Pedro Darío |
| author_browse | Campos, Eleonora Coniglio, Romina O. Diaz, Gabriela V. Fonseca, María I. Ghio, Silvina Ontañon, Ornella Mailén Zapata, Pedro Darío |
| author_facet | Coniglio, Romina O. Fonseca, María I. Diaz, Gabriela V. Ontañon, Ornella Mailén Ghio, Silvina Campos, Eleonora Zapata, Pedro Darío |
| author_sort | Coniglio, Romina O. |
| collection | INTA Digital |
| description | The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to obtain and characterize a cellobiohydrolase-enriched extracellular extract of T. villosa LBM 033 (Misiones, Argentina), which is suitable for the enzymatic hydrolysis of lignocellulosic residues. The effect of carbon and nitrogen sources on cellobiohydrolase activity was evaluated using experimental designs and a culture medium was optimized to obtain a cellobiohydrolase-enriched extract suitable for the hydrolysis of lignocellulosic biomass. Moreover, by secretome analysis, nine enzymes involved in lignocellulosic biomass degradation were identified under the optimized conditions; among them is a cellobiohydrolase II from the glycosil-hydrolase 6 family. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5122 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA51222019-05-16T11:06:56Z Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion Coniglio, Romina O. Fonseca, María I. Diaz, Gabriela V. Ontañon, Ornella Mailén Ghio, Silvina Campos, Eleonora Zapata, Pedro Darío Enzymatic Hydrolysis Biomass Hidrólisis Enzimática Biomasa Trametes Enzimas Lignocelulíticas Bioetanol Lignocellulitic Enzymes Bioethanol The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to obtain and characterize a cellobiohydrolase-enriched extracellular extract of T. villosa LBM 033 (Misiones, Argentina), which is suitable for the enzymatic hydrolysis of lignocellulosic residues. The effect of carbon and nitrogen sources on cellobiohydrolase activity was evaluated using experimental designs and a culture medium was optimized to obtain a cellobiohydrolase-enriched extract suitable for the hydrolysis of lignocellulosic biomass. Moreover, by secretome analysis, nine enzymes involved in lignocellulosic biomass degradation were identified under the optimized conditions; among them is a cellobiohydrolase II from the glycosil-hydrolase 6 family. Instituto de Biotecnología Fil: Coniglio, Romina O. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina Fil: Fonseca, María I. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina Fil: Diaz, Gabriela V. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina 2019-05-16T10:57:40Z 2019-05-16T10:57:40Z 2019-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.tandfonline.com/doi/full/10.1080/25765299.2019.1598107 http://hdl.handle.net/20.500.12123/5122 2576-5299 https://doi.org/10.1080/25765299.2019.1598107 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Arab Journal of Basic and Applied Sciences 26 (1) : 182-192 (Abril 2019) |
| spellingShingle | Enzymatic Hydrolysis Biomass Hidrólisis Enzimática Biomasa Trametes Enzimas Lignocelulíticas Bioetanol Lignocellulitic Enzymes Bioethanol Coniglio, Romina O. Fonseca, María I. Diaz, Gabriela V. Ontañon, Ornella Mailén Ghio, Silvina Campos, Eleonora Zapata, Pedro Darío Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion |
| title | Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion |
| title_full | Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion |
| title_fullStr | Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion |
| title_full_unstemmed | Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion |
| title_short | Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion |
| title_sort | optimization of cellobiohydrolase production and secretome analysis of trametes villosa lbm 033 suitable for lignocellulosic bioconversion |
| topic | Enzymatic Hydrolysis Biomass Hidrólisis Enzimática Biomasa Trametes Enzimas Lignocelulíticas Bioetanol Lignocellulitic Enzymes Bioethanol |
| url | https://www.tandfonline.com/doi/full/10.1080/25765299.2019.1598107 http://hdl.handle.net/20.500.12123/5122 https://doi.org/10.1080/25765299.2019.1598107 |
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