Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On the contrary, BVs poorly infect endothelial gut cells, but propagate the infect...
| Main Authors: | , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
S. Karger AG
2019
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| Subjects: | |
| Online Access: | https://www.karger.com/Article/Abstract/324538 http://hdl.handle.net/20.500.12123/5118 https://doi.org/10.1159/000324538 |
| _version_ | 1855483559113916416 |
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| author | Alfonso, Victoria Lopez, Maria Gabriela Carrillo, Elisa Cristina Taboga, Oscar Alberto |
| author_browse | Alfonso, Victoria Carrillo, Elisa Cristina Lopez, Maria Gabriela Taboga, Oscar Alberto |
| author_facet | Alfonso, Victoria Lopez, Maria Gabriela Carrillo, Elisa Cristina Taboga, Oscar Alberto |
| author_sort | Alfonso, Victoria |
| collection | INTA Digital |
| description | Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On the contrary, BVs poorly infect endothelial gut cells, but propagate the infection in the tissues of insects with a high efficiency. P74 is one of the most important proteins from ODVs, and it participates in the attachment of this viral phenotype to endothelial cells in the midgut. We evaluated the possibility of pseudotyping BVs of Autographa californica multiple nucleopolyhedrovirus with two versions of P74 and its effect on their oral infectivity. Both recombinant BVs contained P74 and replicated similarly to wild-type viruses. Nevertheless, the presence of P74 on the BV’s surface does not enhance the oral infectivity of this phenotype, suggesting that the presence of P74 in the membrane of budded virions interferes with their mechanism of infecting midgut cells. |
| format | Artículo |
| id | INTA5118 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | S. Karger AG |
| publisherStr | S. Karger AG |
| record_format | dspace |
| spelling | INTA51182019-05-15T13:58:30Z Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses Alfonso, Victoria Lopez, Maria Gabriela Carrillo, Elisa Cristina Taboga, Oscar Alberto Virus Baculoviridae Lepidoptera Autographa californica Patogenicidad Fenotipos Viruses Pathogenicity Phenotypes Poder infeccioso Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On the contrary, BVs poorly infect endothelial gut cells, but propagate the infection in the tissues of insects with a high efficiency. P74 is one of the most important proteins from ODVs, and it participates in the attachment of this viral phenotype to endothelial cells in the midgut. We evaluated the possibility of pseudotyping BVs of Autographa californica multiple nucleopolyhedrovirus with two versions of P74 and its effect on their oral infectivity. Both recombinant BVs contained P74 and replicated similarly to wild-type viruses. Nevertheless, the presence of P74 on the BV’s surface does not enhance the oral infectivity of this phenotype, suggesting that the presence of P74 in the membrane of budded virions interferes with their mechanism of infecting midgut cells. Instituto de Biotecnología Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-05-15T13:53:09Z 2019-05-15T13:53:09Z 2012-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.karger.com/Article/Abstract/324538 http://hdl.handle.net/20.500.12123/5118 0300-5526 1423-0100 https://doi.org/10.1159/000324538 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf S. Karger AG Intervirology 55 (3) : 247–251. (February 2012) |
| spellingShingle | Virus Baculoviridae Lepidoptera Autographa californica Patogenicidad Fenotipos Viruses Pathogenicity Phenotypes Poder infeccioso Alfonso, Victoria Lopez, Maria Gabriela Carrillo, Elisa Cristina Taboga, Oscar Alberto Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses |
| title | Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses |
| title_full | Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses |
| title_fullStr | Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses |
| title_full_unstemmed | Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses |
| title_short | Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses |
| title_sort | surface display of acmnpv occlusion derived p74 does not enhance oral infectivity of budded viruses |
| topic | Virus Baculoviridae Lepidoptera Autographa californica Patogenicidad Fenotipos Viruses Pathogenicity Phenotypes Poder infeccioso |
| url | https://www.karger.com/Article/Abstract/324538 http://hdl.handle.net/20.500.12123/5118 https://doi.org/10.1159/000324538 |
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