AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of infected Sf-9 cells, even when ac109 knockout viruses displayed a single-cell inf...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Plos One
2019
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| Subjects: | |
| Online Access: | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046146 http://hdl.handle.net/20.500.12123/5117 https://doi.org/10.1371/journal.pone.0046146 |
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| author | Alfonso, Victoria Maroniche, Guillermo Andrés Reca, Sol Rita Lopez, Maria Gabriela Del Vas, Mariana Taboga, Oscar Alberto |
| author_browse | Alfonso, Victoria Del Vas, Mariana Lopez, Maria Gabriela Maroniche, Guillermo Andrés Reca, Sol Rita Taboga, Oscar Alberto |
| author_facet | Alfonso, Victoria Maroniche, Guillermo Andrés Reca, Sol Rita Lopez, Maria Gabriela Del Vas, Mariana Taboga, Oscar Alberto |
| author_sort | Alfonso, Victoria |
| collection | INTA Digital |
| description | The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of infected Sf-9 cells, even when ac109 knockout viruses displayed a single-cell infection phenotype. Moreover, confocal microscopy analysis revealed that budded virions can enter the cytoplasm but are unable to enter the cell nucleus. This defect could be repaired by complementing ac109 in trans. In addition, polyhedra of normal size could be detected in Sf-9 nuclei infected with ac109 knockout viruses. However, electron microscopy demonstrated that these occlusion bodies were empty. Altogether, these results indicate that ac109 is required for infectivity of both phenotypes of virus. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5117 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Plos One |
| publisherStr | Plos One |
| record_format | dspace |
| spelling | INTA51172019-05-15T13:36:10Z AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny Alfonso, Victoria Maroniche, Guillermo Andrés Reca, Sol Rita Lopez, Maria Gabriela Del Vas, Mariana Taboga, Oscar Alberto Lepidoptera Autographa californica Genética Fenotipos Virus Genetics Phenotypes Viruses The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of infected Sf-9 cells, even when ac109 knockout viruses displayed a single-cell infection phenotype. Moreover, confocal microscopy analysis revealed that budded virions can enter the cytoplasm but are unable to enter the cell nucleus. This defect could be repaired by complementing ac109 in trans. In addition, polyhedra of normal size could be detected in Sf-9 nuclei infected with ac109 knockout viruses. However, electron microscopy demonstrated that these occlusion bodies were empty. Altogether, these results indicate that ac109 is required for infectivity of both phenotypes of virus. Instituto de Biotecnología Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Reca, Sol Rita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-05-15T13:34:37Z 2019-05-15T13:34:37Z 2012-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046146 http://hdl.handle.net/20.500.12123/5117 1932-6203 https://doi.org/10.1371/journal.pone.0046146 eng info:eu-repograntAgreement/INTA/AERG/232161/AR./Vacunas a subunidades: optimización de la presentación antigénica e inmunomodulación y de los métodos biotecnológicos de producción info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plos One PLoS ONE 7 (9) : e46146. (2012) |
| spellingShingle | Lepidoptera Autographa californica Genética Fenotipos Virus Genetics Phenotypes Viruses Alfonso, Victoria Maroniche, Guillermo Andrés Reca, Sol Rita Lopez, Maria Gabriela Del Vas, Mariana Taboga, Oscar Alberto AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny |
| title | AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny |
| title_full | AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny |
| title_fullStr | AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny |
| title_full_unstemmed | AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny |
| title_short | AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny |
| title_sort | acmnpv core gene ac109 is required for budded virion transport to the nucleus and for occlusion of viral progeny |
| topic | Lepidoptera Autographa californica Genética Fenotipos Virus Genetics Phenotypes Viruses |
| url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046146 http://hdl.handle.net/20.500.12123/5117 https://doi.org/10.1371/journal.pone.0046146 |
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