Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
This study investigated the use of previously superovulated ovaries as a source of oocytes, assessing the competence of them for in vitro embryo production. Two superovulatory treatments were performed: equine Chorionic Gonadotrophin (eCG) plus porcine Follicle-Stimulating Hormone (pFSH) in a single...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Taylor & Francis
2019
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| Materias: | |
| Acceso en línea: | https://www.tandfonline.com/doi/full/10.1080/09712119.2011.622929 http://hdl.handle.net/20.500.12123/5073 https://doi.org/10.1080/09712119.2011.622929 |
| _version_ | 1855035434034266112 |
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| author | Forcada, Fernando Buffoni, Andres Abecia, José Alfonso Asenjo, B. Palacin, José Ignacio Vázquez, M.I. Rodriguez Castillo, José del Carmen Sanchez Prieto, L. Casao, A. |
| author_browse | Abecia, José Alfonso Asenjo, B. Buffoni, Andres Casao, A. Forcada, Fernando Palacin, José Ignacio Rodriguez Castillo, José del Carmen Sanchez Prieto, L. Vázquez, M.I. |
| author_facet | Forcada, Fernando Buffoni, Andres Abecia, José Alfonso Asenjo, B. Palacin, José Ignacio Vázquez, M.I. Rodriguez Castillo, José del Carmen Sanchez Prieto, L. Casao, A. |
| author_sort | Forcada, Fernando |
| collection | INTA Digital |
| description | This study investigated the use of previously superovulated ovaries as a source of oocytes, assessing the competence of them for in vitro embryo production. Two superovulatory treatments were performed: equine Chorionic Gonadotrophin (eCG) plus porcine Follicle-Stimulating Hormone (pFSH) in a single dose or the conventional protocol of six decreasing doses of pFSH. Thirty donor ewes of the endangered Ojalada breed were given either the simplified (group S; n=15) or the decreasing-dose (group D; n=15) treatments three times at intervals of ≥50 days. Ovaries were recovered on day 7 after the oestrus following the third treatment, just after embryo flushing, and the oocytes were collected to assess in vitro maturation, fertilisation and development to the blastocyst stage. The two superovulatory treatments did not differ in the mean number of oocytes selected for maturation (7.1±1.2 and 8.5±1.5 per ewe in the D and S groups, respectively). The oocytes recovered from ewes in Group D (87.5%) had a significantly (p<0.05) higher maturation rate than did those recovered from ewes in group S (75%), but no differences were found in fertilisation rate (94% and 94.6% in the D and S groups, respectively); both groups did not differ in their blastocyst rates and the total number of cells in in vitro-produced blastocysts. In the two experimental groups, 1.7 (D) and 1.8 (S) in vitro-produced blastocysts were generated per ewe, which indicate that it is feasible to combine in vivo and in vitro techniques to maximise embryo production in endangered sheep breeds. |
| format | info:ar-repo/semantics/artículo |
| id | INTA5073 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA50732019-05-08T14:22:11Z Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques Forcada, Fernando Buffoni, Andres Abecia, José Alfonso Asenjo, B. Palacin, José Ignacio Vázquez, M.I. Rodriguez Castillo, José del Carmen Sanchez Prieto, L. Casao, A. Ovinos Razas (animales) Conservación de Embriones Experimentación in Vitro Experimentación in Vivo Superovulación Sheep Breeds (animals) Embryo Preservation In Vitro Experimentation In Vivo Experimentation Superovulation This study investigated the use of previously superovulated ovaries as a source of oocytes, assessing the competence of them for in vitro embryo production. Two superovulatory treatments were performed: equine Chorionic Gonadotrophin (eCG) plus porcine Follicle-Stimulating Hormone (pFSH) in a single dose or the conventional protocol of six decreasing doses of pFSH. Thirty donor ewes of the endangered Ojalada breed were given either the simplified (group S; n=15) or the decreasing-dose (group D; n=15) treatments three times at intervals of ≥50 days. Ovaries were recovered on day 7 after the oestrus following the third treatment, just after embryo flushing, and the oocytes were collected to assess in vitro maturation, fertilisation and development to the blastocyst stage. The two superovulatory treatments did not differ in the mean number of oocytes selected for maturation (7.1±1.2 and 8.5±1.5 per ewe in the D and S groups, respectively). The oocytes recovered from ewes in Group D (87.5%) had a significantly (p<0.05) higher maturation rate than did those recovered from ewes in group S (75%), but no differences were found in fertilisation rate (94% and 94.6% in the D and S groups, respectively); both groups did not differ in their blastocyst rates and the total number of cells in in vitro-produced blastocysts. In the two experimental groups, 1.7 (D) and 1.8 (S) in vitro-produced blastocysts were generated per ewe, which indicate that it is feasible to combine in vivo and in vitro techniques to maximise embryo production in endangered sheep breeds. EEA Chubut Fil: Forcada, Fernando. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España Fil: Buffoni, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina Fil: Abecia, José Alfonso. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España Fil: Asenjo, B. Universidad de Valladolid. Escuela Universitaria de Ingenierías Agrarias de Soria; España Fil: Palacin, José Ignacio. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España Fil: Vázquez, M.I. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España Fil: Rodriguez Castillo, José del Carmen. Benemérita Universidad Autónoma de Puebla. Facultad de Medicina Veterinaria y Zootecnia; México Fil: Sanchez Prieto, L. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España Fil: Casao, A. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España 2019-05-08T14:17:03Z 2019-05-08T14:17:03Z 2011 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.tandfonline.com/doi/full/10.1080/09712119.2011.622929 http://hdl.handle.net/20.500.12123/5073 0971-2119 0974-1844 https://doi.org/10.1080/09712119.2011.622929 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Journal of Applied Animal Research 39 (4) : 412-417 (2011) |
| spellingShingle | Ovinos Razas (animales) Conservación de Embriones Experimentación in Vitro Experimentación in Vivo Superovulación Sheep Breeds (animals) Embryo Preservation In Vitro Experimentation In Vivo Experimentation Superovulation Forcada, Fernando Buffoni, Andres Abecia, José Alfonso Asenjo, B. Palacin, José Ignacio Vázquez, M.I. Rodriguez Castillo, José del Carmen Sanchez Prieto, L. Casao, A. Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques |
| title | Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques |
| title_full | Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques |
| title_fullStr | Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques |
| title_full_unstemmed | Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques |
| title_short | Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques |
| title_sort | maximising embryo production in endangered sheep breeds in vitro procedures that complement in vivo techniques |
| topic | Ovinos Razas (animales) Conservación de Embriones Experimentación in Vitro Experimentación in Vivo Superovulación Sheep Breeds (animals) Embryo Preservation In Vitro Experimentation In Vivo Experimentation Superovulation |
| url | https://www.tandfonline.com/doi/full/10.1080/09712119.2011.622929 http://hdl.handle.net/20.500.12123/5073 https://doi.org/10.1080/09712119.2011.622929 |
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